小鼠大脑的慢性成像：从光学系统到功能超声波

Q2 Neuroscience Current Protocols in Neuroscience Pub Date : 2020-09-01 DOI:10.1002/cpns.98

Kıvılcım Kılıç, Jianbo Tang, Ş Evren Erdener, Smrithi Sunil, John T Giblin, Blaire S Lee, Dmitry D Postnov, Anderson Chen, David A Boas

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摘要

功能超声（fUS）在脑血管成像中的应用越来越受到神经科学家的青睐。在本文中，我们介绍了一种可进行纵向研究的慢性手术准备方法，因此适用于广泛的研究，尤其是有关衰老、中风和神经退行性疾病的研究。这种方法也可用于清醒的小鼠，因此可以避免麻醉对神经血管反应的有害影响。除了 fUS 成像，这种手术准备方法还能让研究人员利用常见的光学成像方法获取补充数据集，帮助提高研究的技术严谨性。© 2020 Wiley Periodicals LLC.基本方案 1：使用聚甲基戊烯对小鼠慢性颅窗进行手术准备基本方案 2：对带有慢性颅窗的小鼠进行成像。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Chronic Imaging of Mouse Brain: From Optical Systems to Functional Ultrasound.

Utilization of functional ultrasound (fUS) in cerebral vascular imaging is gaining popularity among neuroscientists. In this article, we describe a chronic surgical preparation method that allows longitudinal studies and therefore is applicable to a wide range of studies, especially on aging, stroke, and neurodegenerative diseases. This method can also be used with awake mice; hence, the deleterious effects of anesthesia on neurovascular responses can be avoided. In addition to fUS imaging, this surgical preparation allows researchers to take advantage of common optical imaging methods to acquire complementary datasets to help increase the technical rigor of studies. © 2020 Wiley Periodicals LLC. Basic Protocol 1: Surgical preparation of mouse chronic cranial windows using polymethylpentene Basic Protocol 2: Imaging of mice with chronic cranial windows.

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期刊介绍： Current Protocols in Neuroscience is a one-stop resource for finding and adapting the best models and methods for all types of neuroscience experiments. Updated every three months in all formats, CPNS is constantly evolving to keep pace with the very latest discoveries and developments. A year of these quarterly updates is included in the initial CPNS purchase price.