海藻糖稳定的冻干犬血小板产品中细胞因子浓度的评价:初步研究。

IF 1.3 Q2 VETERINARY SCIENCES Veterinary Record Open Pub Date : 2020-08-07 eCollection Date: 2020-01-01 DOI:10.1136/vetreco-2019-000366
Robert Goggs, Signe Cremer, Marjory B Brooks
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引用次数: 2

摘要

背景:血小板输注适用于严重血小板减少引起的出血和创伤相关凝血病。发热性非溶血性输血反应是血小板输注的常见并发症,可能是由于炎症细胞因子的积累。本研究旨在确定重建后新型犬冻干血小板产品的细胞因子谱,评估冻干血小板对生理性血小板激动剂的激活反应,并比较基础和刺激犬冻干血小板的细胞因子谱。方法:重组后进行细胞计数和生化分析。细胞因子浓度用犬特异性多重免疫捕获法和电化学发光ELISA法测定。在非搅拌条件下,使用二磷酸腺苷、凝血酶或惊风素及其细胞因子浓度与未激活样品进行比较,将三个单独小瓶的等份重组产物激活10分钟。用流式细胞术和光透射聚集法来评估产品表达促凝剂表面、脱粒和聚集的能力。新鲜富血小板血浆作为阳性对照。结果:该产品的平均±SD颗粒计数为1.23±0.2×109/ml,在激动剂刺激前含有表达表面磷脂酰丝氨酸的血小板,并且在凝血酶刺激下能够聚集,表明该产品在体内给药后可能具有止血潜力。免疫捕获法测量的细胞因子浓度通常较低,而使用ELISA检测的几种细胞因子相对于公布的时间间隔增加了两到三倍。ELISA检测趋化因子(C-X-C)基序配体8和肿瘤坏死因子-α的浓度显著升高,但免疫捕获法检测没有,而免疫捕获法检测kc样蛋白的浓度显著升高。血小板激动剂刺激不影响测量的细胞因子浓度。结论:进一步研究冻干血小板产品的作用是必要的。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Evaluation of cytokine concentrations in a trehalose-stabilised lyophilised canine platelet product: a preliminary study.

Background: Platelet transfusion is indicated for haemorrhage due to severe thrombocytopenia and for trauma associated coagulopathy. Febrile non-haemolytic transfusion reactions are a common complication of platelet transfusions in people and may be due to accumulated inflammatory cytokines. The present study aimed to determine the cytokine profile of a novel canine lyophilised platelet product following reconstitution, to assess the lyophilised platelets' activation response to physiological platelet agonists and to compare the cytokine profiles of basal and stimulated canine lyophilised platelets.

Methods: Cell counts and biochemical analyses were conducted following reconstitution. Cytokine concentrations were measured with a canine-specific multiplex immunocapture assay and with an electrochemiluminescent ELISA. Aliquots of reconstituted product from three separate vials were activated for 10 minutes under non-stirred conditions using adenosine diphosphate, thrombin or convulxin and their cytokine concentrations compared with unactivated samples. Flow cytometry and light-transmission aggregometry were used to evaluate the product's ability to express a procoagulant surface, degranulate and aggregate. Fresh platelet-rich plasma was used as a positive control.

Results: The product had a mean±SD particle count of 1.23±0.2×109/ml, contained platelets that expressed surface phosphatidylserine before agonist stimulation and was capable of aggregation in response to thrombin stimulation suggesting that the product may have haemostatic potential following in vivo administration. Cytokine concentrations measured by the immunocapture assay were generally low, while twofold to threefold increases relative to published intervals were noted for several cytokines using the ELISA. Concentrations of chemokine (C-X-C) motif ligand 8 and tumour necrosis factor-α were significantly increased as measured by the ELISA, but not by the immunocapture assay, while concentrations of KC-like were significantly increased as measured by the immunocapture assay. Stimulation with platelet agonists did not affect measured cytokine concentrations.

Conclusion: Further study of the effects of administration of this lyophilised platelet product is warranted.

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来源期刊
Veterinary Record Open
Veterinary Record Open VETERINARY SCIENCES-
CiteScore
3.00
自引率
0.00%
发文量
25
审稿时长
19 weeks
期刊介绍: Veterinary Record Open is a journal dedicated to publishing specialist veterinary research across a range of topic areas including those of a more niche and specialist nature to that considered in the weekly Vet Record. Research from all disciplines of veterinary interest will be considered. It is an Open Access journal of the British Veterinary Association.
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