{"title":"利用发光二极管和亚甲基蓝杀菌大肠杆菌。","authors":"Kouji Ogasawara, Susumu Nakajima, Hiroshi Sato, Tadashi Sasaki","doi":"10.5978/islsm.19-OR-05","DOIUrl":null,"url":null,"abstract":"<p><strong>Background and aims: </strong>The prevalence of pathogenic E<i>scherichia coli</i> as well as <i>E. coli</i> O157 and antibiotic- resistant bacteria has increased. This study aimed to examine the effect of methylene blue (MB) with sodium bicarbonate (NaHCO<sub>3</sub>) against <i>E. coli</i> using photodynamic antimicrobial chemotherapy (PACT).</p><p><strong>Materials and methods: </strong>MB was basified using NaHCO<sub>3</sub>. <i>E. coli</i> and basic MB were smeared on the culture media followed by irradiation using a red light-emitting diode (LED) at 660 nm. Energy densities of 5, 10, 15, and 20 J/cm<sup>2</sup> were applied to the culture medium.After 24 h, the bactericidal effect of basic MB with LED irradiation was determined based on the bacterial growth.</p><p><strong>Results: </strong>The basic effect was observed with 1%-6% of NaHCO<sub>3</sub> at 5 J/cm<sup>2</sup>.This effect increased between 1% and 2% of NaHCO<sub>3</sub> at 10 J/cm<sup>2</sup> and 15 J/cm<sup>2</sup>, whereas decreased at the NaHCO<sub>3</sub> concentrations of > 2%. Moreover, this effect decreased at an energy density of 20 J/cm<sup>2</sup>. The biphasic basic effect on bactericidal activity was observed between pH 8.6-9.0.</p><p><strong>Conclusions: </strong>Thus, PACT using basic MB may be an effective method for pathogenic <i>E. coli</i> sterilization.</p>","PeriodicalId":17981,"journal":{"name":"Laser therapy","volume":"28 2","pages":"97-102"},"PeriodicalIF":0.0000,"publicationDate":"2019-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7456653/pdf/islsm-28-97.pdf","citationCount":"0","resultStr":"{\"title\":\"<i>Escherichia coli</i> Sterilization Using a Light-Emitting Diode and Methylene Blue.\",\"authors\":\"Kouji Ogasawara, Susumu Nakajima, Hiroshi Sato, Tadashi Sasaki\",\"doi\":\"10.5978/islsm.19-OR-05\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background and aims: </strong>The prevalence of pathogenic E<i>scherichia coli</i> as well as <i>E. coli</i> O157 and antibiotic- resistant bacteria has increased. This study aimed to examine the effect of methylene blue (MB) with sodium bicarbonate (NaHCO<sub>3</sub>) against <i>E. coli</i> using photodynamic antimicrobial chemotherapy (PACT).</p><p><strong>Materials and methods: </strong>MB was basified using NaHCO<sub>3</sub>. <i>E. coli</i> and basic MB were smeared on the culture media followed by irradiation using a red light-emitting diode (LED) at 660 nm. Energy densities of 5, 10, 15, and 20 J/cm<sup>2</sup> were applied to the culture medium.After 24 h, the bactericidal effect of basic MB with LED irradiation was determined based on the bacterial growth.</p><p><strong>Results: </strong>The basic effect was observed with 1%-6% of NaHCO<sub>3</sub> at 5 J/cm<sup>2</sup>.This effect increased between 1% and 2% of NaHCO<sub>3</sub> at 10 J/cm<sup>2</sup> and 15 J/cm<sup>2</sup>, whereas decreased at the NaHCO<sub>3</sub> concentrations of > 2%. Moreover, this effect decreased at an energy density of 20 J/cm<sup>2</sup>. The biphasic basic effect on bactericidal activity was observed between pH 8.6-9.0.</p><p><strong>Conclusions: </strong>Thus, PACT using basic MB may be an effective method for pathogenic <i>E. coli</i> sterilization.</p>\",\"PeriodicalId\":17981,\"journal\":{\"name\":\"Laser therapy\",\"volume\":\"28 2\",\"pages\":\"97-102\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2019-06-30\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7456653/pdf/islsm-28-97.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Laser therapy\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.5978/islsm.19-OR-05\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"Medicine\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Laser therapy","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.5978/islsm.19-OR-05","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"Medicine","Score":null,"Total":0}
Escherichia coli Sterilization Using a Light-Emitting Diode and Methylene Blue.
Background and aims: The prevalence of pathogenic Escherichia coli as well as E. coli O157 and antibiotic- resistant bacteria has increased. This study aimed to examine the effect of methylene blue (MB) with sodium bicarbonate (NaHCO3) against E. coli using photodynamic antimicrobial chemotherapy (PACT).
Materials and methods: MB was basified using NaHCO3. E. coli and basic MB were smeared on the culture media followed by irradiation using a red light-emitting diode (LED) at 660 nm. Energy densities of 5, 10, 15, and 20 J/cm2 were applied to the culture medium.After 24 h, the bactericidal effect of basic MB with LED irradiation was determined based on the bacterial growth.
Results: The basic effect was observed with 1%-6% of NaHCO3 at 5 J/cm2.This effect increased between 1% and 2% of NaHCO3 at 10 J/cm2 and 15 J/cm2, whereas decreased at the NaHCO3 concentrations of > 2%. Moreover, this effect decreased at an energy density of 20 J/cm2. The biphasic basic effect on bactericidal activity was observed between pH 8.6-9.0.
Conclusions: Thus, PACT using basic MB may be an effective method for pathogenic E. coli sterilization.
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