应激状态下海马去甲肾上腺素能能力的变化。

IF 1.1 4区医学 Q3 BIOLOGY Folia Biologica Pub Date : 2020-01-01 DOI:10.14712/fb2020066020081

L Gavrilović, N Popović, V Stojiljković, S Pejić, A Todorović, I Pavlović, S B Pajović

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引用次数: 0

摘要

本研究旨在探讨慢性约束应激(CRS)对大鼠海马多巴胺-β-羟化酶(DBH)、去甲肾上腺素转运蛋白(NET)、囊泡单胺转运蛋白2 (VMAT2)和脑源性神经营养因子(BDNF)蛋白水平及去甲肾上腺素(NA)浓度的影响。采用免疫印迹法(Western blot)和酶联免疫吸附试验(ELISA)对各指标进行定量分析。我们发现，CRS使慢性应激大鼠海马中DBH蛋白水平提高30%，VMAT2蛋白水平提高11%，BDNF蛋白水平提高11%，NA浓度提高104%，而NET蛋白水平降低16%。CRS提高海马NA水平的分子机制是应激条件下去肾上腺素能系统的重要适应现象。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Changes of Hippocampal Noradrenergic Capacity in Stress Condition.

This study aimed to investigate the effects of chronic restraint stress (CRS) on the protein levels of dopamine-β-hydroxylase (DBH), noradrenaline transporter (NET), vesicular monoamine transporter 2 (VMAT2) and brain-derived neurotrophic factor (BDNF), as well as the concentration of noradrenaline (NA) in the rat hippocampus. The investigated parameters were quantified by Western blot analyses and ELISA kits. We found that CRS increased the protein levels of DBH by 30 %, VMAT2 by 11 %, BDNF by 11 % and the concentration of NA by 104 %, but decreased the protein levels of NET by 16 % in the hippocampus of chronically stressed rats. The molecular mechanisms by which CRS increased the hippocampal NA level are an important adaptive phenomenon of the noradrenergic system in the stress condition.

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来源期刊

Folia Biologica 医学-生物学

CiteScore

1.40

自引率

0.00%

发文量

审稿时长

3 months

期刊介绍： Journal of Cellular and Molecular Biology publishes articles describing original research aimed at the elucidation of a wide range of questions of biology and medicine at the cellular and molecular levels. Studies on all organisms as well as on human cells and tissues are welcome.