不同血糖方案下乳腺癌细胞的生物力学和生物物理特性。

IF 1.8 Q3 ONCOLOGY Breast Cancer : Basic and Clinical Research Pub Date : 2020-11-12 eCollection Date: 2020-01-01 DOI:10.1177/1178223420972362
Diganta Dutta, Xavier-Lewis Palmer, Jose Ortega-Rodas, Vasundhara Balraj, Indrani Ghosh Dastider, Surabhi Chandra
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引用次数: 6

摘要

糖尿病会加速癌细胞的增殖和转移,尤其是胰腺癌、肝癌、乳腺癌、结肠癌和皮肤癌。虽然这两种疾病之间的联系途径已经被广泛探索,但关于葡萄糖是否会引起细胞结构变化,从而使癌细胞易产生侵袭性表型,目前还缺乏相关信息。因此,假设暴露于糖尿病/高糖环境中,癌细胞的生物力学和生物物理特性比正常细胞更能改变,这有助于癌症的发展。本研究采用原子力显微镜压痕法,对不同血糖应激水平下的多种人乳腺癌细胞(MCF-7、MDA-MB-231)和人正常乳腺上皮细胞(MCF-10A)进行微尺度探测。这些被用来研究良性和恶性乳腺组织的行为。在正常血糖条件下,良性细胞(MCF-10A)的杨氏模量值高于恶性细胞(MCF-7和MDA-231),这与目前的文献一致。此外,暴露于高葡萄糖(48小时)降低了良性和恶性细胞的杨氏模量,结果是癌细胞在高葡萄糖下完全失去弹性。这为血糖应激和细胞骨架强度之间的联系提供了可能的见解。这项研究表明,降低癌症患者和高危人群的血糖压力有助于恢复正常的细胞骨架结构。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Biomechanical and Biophysical Properties of Breast Cancer Cells Under Varying Glycemic Regimens.

Diabetes accelerates cancer cell proliferation and metastasis, particularly for cancers of the pancreas, liver, breast, colon, and skin. While pathways linking the 2 disease conditions have been explored extensively, there is a lack of information on whether there could be cytoarchitectural changes induced by glucose which predispose cancer cells to aggressive phenotypes. It was thus hypothesized that exposure to diabetes/high glucose alters the biomechanical and biophysical properties of cancer cells more than the normal cells, which aids in advancing the cancer. For this study, atomic force microscopy indentation was used through microscale probing of multiple human breast cancer cells (MCF-7, MDA-MB-231), and human normal mammary epithelial cells (MCF-10A), under different levels of glycemic stress. These were used to study both benign and malignant breast tissue behaviors. Benign cells (MCF-10A) recorded higher Young's modulus values than malignant cells (MCF-7 and MDA-231) under normoglycemic conditions, which agrees with the current literature. Moreover, exposure to high glucose (for 48 hours) decreased Young's modulus in both benign and malignant cells, to the effect that the cancer cells showed a complete loss in elasticity with high glucose. This provides a possible insight into a link between glycemic stress and cytoskeletal strength. This work suggests that reducing glycemic stress in cancer patients and those at risk can prove beneficial in restoring normal cytoskeletal structure.

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来源期刊
CiteScore
5.10
自引率
3.40%
发文量
22
审稿时长
8 weeks
期刊介绍: Breast Cancer: Basic and Clinical Research is an international, open access, peer-reviewed, journal which considers manuscripts on all areas of breast cancer research and treatment. We welcome original research, short notes, case studies and review articles related to breast cancer-related research. Specific areas of interest include, but are not limited to, breast cancer sub types, pathobiology, metastasis, genetics and epigenetics, mammary gland biology, breast cancer models, prevention, detection, therapy and clinical interventions, and epidemiology and population genetics.
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