使用全长 tau 底物 RT-QuIC 检测 tauopathies。

IF 18 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY Accounts of Chemical Research Pub Date : 2020-12-01 DOI:10.1080/19336896.2020.1832946

Joanne M Tennant, Davin M Henderson, Thomas M Wisniewski, Edward A Hoover

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引用次数: 0

摘要

神经退行性疾病的早期检测和诊断因缺乏灵敏的检测手段而受到阻碍。实时震荡诱导转换（RT-QuIC）已被用于早期灵敏检测朊病毒诱导的神经系统疾病，最近又被用于检测作为神经退行性疾病生物标志物的折叠错误的α-突触核蛋白和tau。在这里，我们使用全长重组 tau 底物来检测阿尔茨海默病和其他人类 tau 病的 tau 播种活性。

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RT-QuIC detection of tauopathies using full-length tau substrates.

Early detection and diagnosis of neurodegenerative diseases has been hampered by the lack of sensitive testing. Real-time quaking induced conversion (RT-QuIC) has been used for the early and sensitive detection of prion-induced neurologic disease, and has more recently been adapted to detect misfolded alpha-synuclein and tau as biomarkers for neurodegenerative disease. Here we use full-length recombinant tau substrates to detect tau seeding activity in Alzheimer's disease and other human tauopathies.

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.