胸腺内皮细胞和胸腺细胞之间的相互作用受生长激素的影响。

IF 1.8 4区 生物学 Q4 CELL BIOLOGY Growth factors Pub Date : 2020-06-01 Epub Date: 2021-05-24 DOI:10.1080/08977194.2021.1924699
Marvin Paulo Lins, Iana Mayane Mendes Nicácio Viana, Salete Smaniotto, Maria Danielma Dos Santos Reis
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引用次数: 3

摘要

生长激素(GH),除了其对体内生长和代谢的经典作用外,还对免疫系统,特别是胸腺产生多效性作用。本研究旨在探讨生长激素对参与迁移过程的成熟胸腺细胞与胸腺内皮相互作用的影响。为此,采用新鲜胸腺细胞(C57BL/6小鼠)和胸腺内皮细胞系(tEnd.1)。在细胞粘附实验中,gh处理的胸腺细胞对tEnd的粘附更强。1细胞。此外,tEnd对纤维连接蛋白的沉积有改善作用。与gh预处理的胸腺细胞共培养1个细胞。此外,生长激素诱导胸腺细胞f -肌动蛋白聚合。在跨内皮迁移实验中,gh处理的大量胸腺细胞,主要是CD4-CD8+亚群,在胰岛素样生长因子1的刺激下向内皮迁移。总之,我们证明了生长激素在胸腺细胞/胸腺内皮相互作用中的积极作用,包括跨内皮迁移。
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Interactions between thymic endothelial cells and thymocytes are influenced by growth hormone.

Growth hormone (GH), in addition to its classic actions on growth and metabolism in the body, exerts pleiotropic effects on the immune system, particularly on the thymus. The aim of this study was to evaluate the influence of GH on the interactions between mature thymocytes and the thymic endothelium involved in the migratory process. To this end, fresh thymocytes (C57BL/6 mice) and the thymic endothelial cell line (tEnd.1) were used. In the cell adhesion assay, the GH-treated thymocytes adhered more to tEnd.1 cells. Additionally, there was an improvement in the deposition of fibronectin by tEnd.1 cells when co-cultured with GH-pre-treated thymocytes. Furthermore, GH induced thymocyte F-actin polymerization. In the transendothelial migration assay, a large number of GH-treated thymocytes, mainly the CD4-CD8+ subset, migrated towards the endothelium under the stimulus of insulin-like growth factor 1. In conclusion, we demonstrated the positive actions of GH in thymocyte/thymic endothelium interactions, including transendothelial migration.

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来源期刊
Growth factors
Growth factors 生物-内分泌学与代谢
CiteScore
2.60
自引率
0.00%
发文量
20
审稿时长
>12 weeks
期刊介绍: Growth Factors is an international and interdisciplinary vehicle publishing new knowledge and findings on the regulators of cell proliferation, differentiation and survival. The Journal will publish research papers, short communications and reviews on current developments in cell biology, biochemistry, physiology or pharmacology of growth factors, cytokines or hormones which improve our understanding of biology or medicine. Among the various fields of study topics of particular interest include: •Stem cell biology •Growth factor physiology •Structure-activity relationships •Drug development studies •Clinical applications
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