共表达Eranthis hyemalis溶血磷脂酸酰基转移酶2和延长酶提高了两种超长链多不饱和脂肪酸的产量

IF 3.7 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Metabolic Engineering Communications Pub Date : 2021-06-01 DOI:10.1016/j.mec.2021.e00171
Dauenpen Meesapyodsuk , Yi Chen , Shengjian Ye , Robert G. Chapman , Xiao Qiu
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引用次数: 5

摘要

二十二碳二烯酸(DDA, 22:2 - 13,16)和二十二碳三烯酸(DTA, 22:3 - 13,16,19)是两种非常长链的多不饱和脂肪酸(VLCPUFAs),最近被证明具有很强的抗炎和抗肿瘤特性。野生植物叶毛花ELO型延长酶EhELO1可以分别通过亚油酸和α -亚麻酸的顺序延长合成这两种脂肪酸。该基因在油料作物芸苔(Brassica carinata)中的种子特异性表达可在转基因种子中产生大量的DDA和DTA。然而,这些脂肪酸被排除在三酰基甘油(TAGs)的sn-2位置之外。为了提高转基因油籽作物中VLCPUFAs的生产水平和营养价值,从叶毛孢中鉴定出一种细胞质溶血磷脂酸酰基转移酶(EhLPAAT2),该酶能将这两种脂肪酸转移到三酰基甘油的n-2位置。RT-PCR分析结果显示,EhELO1基因在萌发种子中优先表达,而EhELO1基因仅在胚膜菊中表达。EhLPAAT2和EhELO1的种子特异性表达使DDA和DTA在tag的sn-2位置有效结合,从而增加了转基因种子中DDA和DTA的总量。据我们所知,这是第一个可以将VLCPUFAs整合到tag中的植物LPAAT。利用EhLPAAT2和EhELO1在油籽作物中提高DDA和DTA的产量,为高价值的营养保健农产品提供了真正的商业机会。
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Co-expressing Eranthis hyemalis lysophosphatidic acid acyltransferase 2 and elongase improves two very long chain polyunsaturated fatty acid production in Brassica carinata

Docosadienoic acid (DDA, 22:2–13,16) and docosatrienoic acid (DTA, 22:3–13,16,19) are two very long chain polyunsaturated fatty acids (VLCPUFAs) that are recently shown to possess strong anti-inflammatory and antitumor properties. An ELO type elongase (EhELO1) from wild plant Eranthis hyemalis can synthesize the two fatty acids by sequential elongation of linoleic acid and alpha-linolenic acid, respectively. Seed-specific expression of this gene in oilseed crop Brassica carinata produced a considerable amount of DDA and DTA in transgenic seeds. However, these fatty acids were excluded from the sn-2 position of triacylglycerols (TAGs). To improve the production level and nutrition value of the VLCPUFAs in the transgenic oilseed crop, a cytoplasmic lysophosphatidic acid acyltransferase (EhLPAAT2) for the incorporation of the two fatty acids into the sn-2 position of triacylglycerols was identified from E. hyemalis. RT-PCR analysis showed that it was preferentially expressed in developing seeds where EhELO1 was exclusively expressed in E. hyemalis. Seed specific expression of EhLPAAT2 along with EhELO1 in B. carinata resulted in the effective incorporation of DDA and DTA at the sn-2 position of TAGs, thereby increasing the total amount of DDA and DTA in transgenic seeds. To our knowledge, this is the first plant LPAAT that can incorporate VLCPUFAs into TAGs. Improved production of DDA and DTA in the oilseed crop using EhLPAAT2 and EhELO1 provides a real commercial opportunity for high value agriculture products for nutraceutical uses.

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来源期刊
Metabolic Engineering Communications
Metabolic Engineering Communications Medicine-Endocrinology, Diabetes and Metabolism
CiteScore
13.30
自引率
1.90%
发文量
22
审稿时长
18 weeks
期刊介绍: Metabolic Engineering Communications, a companion title to Metabolic Engineering (MBE), is devoted to publishing original research in the areas of metabolic engineering, synthetic biology, computational biology and systems biology for problems related to metabolism and the engineering of metabolism for the production of fuels, chemicals, and pharmaceuticals. The journal will carry articles on the design, construction, and analysis of biological systems ranging from pathway components to biological complexes and genomes (including genomic, analytical and bioinformatics methods) in suitable host cells to allow them to produce novel compounds of industrial and medical interest. Demonstrations of regulatory designs and synthetic circuits that alter the performance of biochemical pathways and cellular processes will also be presented. Metabolic Engineering Communications complements MBE by publishing articles that are either shorter than those published in the full journal, or which describe key elements of larger metabolic engineering efforts.
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