Madalina Irina Mitran, Mircea Tampa, Ilinca Nicolae, Cristina Iulia Mitran, Clara Matei, Simona Roxana Georgescu, Mircea Ioan Popa
{"title":"扁平地衣氧化应激新标志物及丙型肝炎病毒感染的影响-一项初步研究。","authors":"Madalina Irina Mitran, Mircea Tampa, Ilinca Nicolae, Cristina Iulia Mitran, Clara Matei, Simona Roxana Georgescu, Mircea Ioan Popa","doi":"10.2478/rjim-2021-0017","DOIUrl":null,"url":null,"abstract":"<p><p><b>Introduction.</b> Lichen planus (LP) is a mucocutaneous T-cell mediated disorder of unknown etiology. There is growing evidence that oxidative stress is an important player in the pathogenesis of LP. Therefore, we have investigated oxidative stress markers in LP and the influence of hepatitis C virus (HCV) infection, a frequently associated condition, on oxidative stress in LP patients. <b>Method.</b> We have determined the serum levels of 4- hydroxynonenal (4-HNE) and symmetric dimethylarginine (SDMA), as markers of oxidative stress, and total antioxidant capacity (TAC), as a marker of the antioxidant defence, in 4 groups: group A - HCV positive patients with LP (n=12), group B - HCV positive patients without LP (n=12), group C - HCV negative patients with LP (n=31) and group D - control group (n=26). <b>Results.</b> In LP patients, we have identified an increased level of lipid peroxidation (4-HNE - group A - 8.41±1.11 μg/mL, group B - 7.97±2.17 μg/mL, group C - 7.81±1.96 μg/mL and group D - 6.15±1.17 μg/mL) and alterations in arginine methylation (SDMA - group A - 1.10±0.24 μmol/L, group B - 1.03±0.16 μmol/L, group C - 0.84±0.19 μmol/L and group D - 0.50±0.06 μmol/L) associated with a diminished antioxidant defence (TAC - group A - 234.50±49.96, μmol/L group B - 255.83±41.41 μmol/L, group C - 269.83±43.33 μmol/L and group D - 316.46 ±29.33 μmol/L), processes augmented by the association with HCV infection. <b>Conclusion.</b> There is an imbalance between oxidants and antioxidants in patients with LP, an imbalance that is augmented by the presence of HCV infection. SDMA could be regarded as a novel biomarker of oxidative stress among these patients. To the best of our knowledge this is the first study to investigate the influence of HCV infection on oxidative stress in LP patients.</p>","PeriodicalId":21463,"journal":{"name":"Romanian Journal of Internal Medicine","volume":null,"pages":null},"PeriodicalIF":1.6000,"publicationDate":"2021-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"New markers of oxidative stress in lichen planus and the influence of hepatitis C virus infection - a pilot study.\",\"authors\":\"Madalina Irina Mitran, Mircea Tampa, Ilinca Nicolae, Cristina Iulia Mitran, Clara Matei, Simona Roxana Georgescu, Mircea Ioan Popa\",\"doi\":\"10.2478/rjim-2021-0017\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p><b>Introduction.</b> Lichen planus (LP) is a mucocutaneous T-cell mediated disorder of unknown etiology. There is growing evidence that oxidative stress is an important player in the pathogenesis of LP. Therefore, we have investigated oxidative stress markers in LP and the influence of hepatitis C virus (HCV) infection, a frequently associated condition, on oxidative stress in LP patients. <b>Method.</b> We have determined the serum levels of 4- hydroxynonenal (4-HNE) and symmetric dimethylarginine (SDMA), as markers of oxidative stress, and total antioxidant capacity (TAC), as a marker of the antioxidant defence, in 4 groups: group A - HCV positive patients with LP (n=12), group B - HCV positive patients without LP (n=12), group C - HCV negative patients with LP (n=31) and group D - control group (n=26). <b>Results.</b> In LP patients, we have identified an increased level of lipid peroxidation (4-HNE - group A - 8.41±1.11 μg/mL, group B - 7.97±2.17 μg/mL, group C - 7.81±1.96 μg/mL and group D - 6.15±1.17 μg/mL) and alterations in arginine methylation (SDMA - group A - 1.10±0.24 μmol/L, group B - 1.03±0.16 μmol/L, group C - 0.84±0.19 μmol/L and group D - 0.50±0.06 μmol/L) associated with a diminished antioxidant defence (TAC - group A - 234.50±49.96, μmol/L group B - 255.83±41.41 μmol/L, group C - 269.83±43.33 μmol/L and group D - 316.46 ±29.33 μmol/L), processes augmented by the association with HCV infection. <b>Conclusion.</b> There is an imbalance between oxidants and antioxidants in patients with LP, an imbalance that is augmented by the presence of HCV infection. SDMA could be regarded as a novel biomarker of oxidative stress among these patients. To the best of our knowledge this is the first study to investigate the influence of HCV infection on oxidative stress in LP patients.</p>\",\"PeriodicalId\":21463,\"journal\":{\"name\":\"Romanian Journal of Internal Medicine\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.6000,\"publicationDate\":\"2021-11-20\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Romanian Journal of Internal Medicine\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.2478/rjim-2021-0017\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2021/12/1 0:00:00\",\"PubModel\":\"Print\",\"JCR\":\"Q2\",\"JCRName\":\"MEDICINE, GENERAL & INTERNAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Romanian Journal of Internal Medicine","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.2478/rjim-2021-0017","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2021/12/1 0:00:00","PubModel":"Print","JCR":"Q2","JCRName":"MEDICINE, GENERAL & INTERNAL","Score":null,"Total":0}
New markers of oxidative stress in lichen planus and the influence of hepatitis C virus infection - a pilot study.
Introduction. Lichen planus (LP) is a mucocutaneous T-cell mediated disorder of unknown etiology. There is growing evidence that oxidative stress is an important player in the pathogenesis of LP. Therefore, we have investigated oxidative stress markers in LP and the influence of hepatitis C virus (HCV) infection, a frequently associated condition, on oxidative stress in LP patients. Method. We have determined the serum levels of 4- hydroxynonenal (4-HNE) and symmetric dimethylarginine (SDMA), as markers of oxidative stress, and total antioxidant capacity (TAC), as a marker of the antioxidant defence, in 4 groups: group A - HCV positive patients with LP (n=12), group B - HCV positive patients without LP (n=12), group C - HCV negative patients with LP (n=31) and group D - control group (n=26). Results. In LP patients, we have identified an increased level of lipid peroxidation (4-HNE - group A - 8.41±1.11 μg/mL, group B - 7.97±2.17 μg/mL, group C - 7.81±1.96 μg/mL and group D - 6.15±1.17 μg/mL) and alterations in arginine methylation (SDMA - group A - 1.10±0.24 μmol/L, group B - 1.03±0.16 μmol/L, group C - 0.84±0.19 μmol/L and group D - 0.50±0.06 μmol/L) associated with a diminished antioxidant defence (TAC - group A - 234.50±49.96, μmol/L group B - 255.83±41.41 μmol/L, group C - 269.83±43.33 μmol/L and group D - 316.46 ±29.33 μmol/L), processes augmented by the association with HCV infection. Conclusion. There is an imbalance between oxidants and antioxidants in patients with LP, an imbalance that is augmented by the presence of HCV infection. SDMA could be regarded as a novel biomarker of oxidative stress among these patients. To the best of our knowledge this is the first study to investigate the influence of HCV infection on oxidative stress in LP patients.