KLF17表达上调可增加胃癌对5-氟尿嘧啶的敏感性。

Zhao-Jie An, Yong Li, Bi-Bo Tan, Qun Zhao, Li-Qiao Fan, Zhi-Dong Zhang, Xue-Feng Zhao, Shao-Yi Li
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引用次数: 4

摘要

有报道称kr ppel样因子17 (KLF17)的表达与多种肿瘤的发生、发展、侵袭、转移及化疗耐药有关。然而,KLF17促进胃癌(GC)化疗耐药的具体机制尚未得到充分研究。本研究收集60例胃癌患者的胃癌组织和非肿瘤组织(与胃癌组织相匹配的邻近正常组织),采用qRT-PCR、Western blot和免疫组化等方法分析KLF17的表达与患者临床病理数据的关系。采用MTS、流式细胞术、Western blot检测KLF17对胃癌细胞株5-氟尿嘧啶(5-FU)敏感性的影响,并探讨其可能的作用机制。与非肿瘤组织相比,GC组织中KLF17的表达水平明显下调,GES-1细胞系和GC细胞系中KLF17的表达水平也有类似的趋势。KLF17的下调表达与肿瘤大小、侵袭、区域淋巴结转移和TNM分期有关。此外,通过上调KLF17的表达,BGC-823和SGC-7901细胞株对5-FU的敏感性明显增强。从机制上讲,上调KLF17的表达可抑制p -糖蛋白(P-gp)、多药耐药蛋白1 (MRP1)和b细胞淋巴瘤-2 (BCL-2)的表达,这些蛋白被报道与耐药和细胞增殖有关。综上所述,这些数据提示KLF17具有抑制胃癌化疗耐药的生物学效应,可能是胃癌化疗耐药的潜在策略。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Up-regulation of KLF17 expression increases the sensitivity of gastric cancer to 5-fluorouracil.

It has been reported that the expression of Krüppel-like factor 17 (KLF17) was associated with the occurrence, development, invasion, metastasis and chemotherapy resistance of various tumors. However, the detailed mechanisms by which KLF17 promotes chemotherapy resistance in gastric cancer (GC) have not been fully investigated. In the present study, we collected the GC tissues and non-tumor tissues (matched adjacent normal tissues with corresponding GC tissues) of 60 GC patients, used qRT-PCR, Western blot and immunohistochemistry assay to analyze the relationship between the expression of KLF17 and the clinical pathological data of the patients. The effect of KLF17 on the sensitivity of GC cell lines to 5-fluorouracil (5-FU), and the potential mechanism were detected by MTS assay, Flow cytometry assay, and Western blot. Compared with non-tumor tissues, the expression level of KLF17 in GC tissue was significantly down-regulated, and the expression level of KLF17 in GES-1 cell line and GC cell lines also had a similar trend. Down-regulated expression of KLF17 is related to tumor size, invasion, regional lymph node metastasis, and TNM staging. Furthermore, through upregulating the expression of KLF17, the sensitivity of BGC-823 and SGC-7901 cell lines to 5-FU was obviously increased. Mechanistically, upregulation the expression of KLF17 can inhibit the expressions of P-glycoprotein (P-gp), multidrug resistance protein 1 (MRP1), and B-Cell lymphoma-2 (BCL-2), which have been reported to be associated with drug resistance and cell proliferation. Collectively, these data implied that KLF17 has the biological effect of inhibiting chemotherapy resistance of GC, and it could be a potential strategy for the GC chemotherapy resistance.

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来源期刊
CiteScore
4.00
自引率
0.00%
发文量
88
审稿时长
15 weeks
期刊介绍: International Journal of Immunopathology and Pharmacology is an Open Access peer-reviewed journal publishing original papers describing research in the fields of immunology, pathology and pharmacology. The intention is that the journal should reflect both the experimental and clinical aspects of immunology as well as advances in the understanding of the pathology and pharmacology of the immune system.
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