{"title":"SKF-96365对OVA诱发的小鼠过敏性鼻炎的治疗作用","authors":"Guangyi Ba, Ru Tang, Xiwen Sun, Zhipeng Li, Hai Lin, Weitian Zhang","doi":"10.1177/20587384211015054","DOIUrl":null,"url":null,"abstract":"<p><strong>Introduction: </strong>SKF-96365 is regarded as an inhibitor of receptor-mediated calcium ion (Ca<sup>2+</sup>) entry. The current study aimed to explore the effects of SKF-96365 on murine allergic rhinitis (AR).</p><p><strong>Methods: </strong>Intranasal SKF-96365 administration was performed on OVA induced murine AR. Serum and nasal lavage fluid (NLF) from mice were harvested to assay IgE and inflammatory cytokines using ELISA method. Inflammatory cells were counted and analyzed in NLF. Nasal mucosa tissues were collected from mice and used for HE staining, immunohistochemistry (IHC) staining, and real-time PCR detection.</p><p><strong>Results: </strong>SKF-96365 had therapeutic effects on murine AR manifesting attenuation of sneezing, nasal rubbing, IgE, inflammatory cytokines, inflammatory cells, TRPC6 immunolabeling, and TRPC6, STIM1 and Orai1 mRNA levels in AR mice.</p><p><strong>Conclusion: </strong>SKF-96365 could effectively alleviate the symptoms of murine AR. SKF-96365 could suppress TRPC6, STIM1, and Orai1 activities, leading to the downregulation of inflammatory cytokines and inflammatory cells in murine AR.</p>","PeriodicalId":14046,"journal":{"name":"International Journal of Immunopathology and Pharmacology","volume":"35 ","pages":"20587384211015054"},"PeriodicalIF":3.0000,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/fe/ca/10.1177_20587384211015054.PMC8127738.pdf","citationCount":"0","resultStr":"{\"title\":\"Therapeutic effects of SKF-96365 on murine allergic rhinitis induced by OVA.\",\"authors\":\"Guangyi Ba, Ru Tang, Xiwen Sun, Zhipeng Li, Hai Lin, Weitian Zhang\",\"doi\":\"10.1177/20587384211015054\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Introduction: </strong>SKF-96365 is regarded as an inhibitor of receptor-mediated calcium ion (Ca<sup>2+</sup>) entry. The current study aimed to explore the effects of SKF-96365 on murine allergic rhinitis (AR).</p><p><strong>Methods: </strong>Intranasal SKF-96365 administration was performed on OVA induced murine AR. Serum and nasal lavage fluid (NLF) from mice were harvested to assay IgE and inflammatory cytokines using ELISA method. Inflammatory cells were counted and analyzed in NLF. Nasal mucosa tissues were collected from mice and used for HE staining, immunohistochemistry (IHC) staining, and real-time PCR detection.</p><p><strong>Results: </strong>SKF-96365 had therapeutic effects on murine AR manifesting attenuation of sneezing, nasal rubbing, IgE, inflammatory cytokines, inflammatory cells, TRPC6 immunolabeling, and TRPC6, STIM1 and Orai1 mRNA levels in AR mice.</p><p><strong>Conclusion: </strong>SKF-96365 could effectively alleviate the symptoms of murine AR. SKF-96365 could suppress TRPC6, STIM1, and Orai1 activities, leading to the downregulation of inflammatory cytokines and inflammatory cells in murine AR.</p>\",\"PeriodicalId\":14046,\"journal\":{\"name\":\"International Journal of Immunopathology and Pharmacology\",\"volume\":\"35 \",\"pages\":\"20587384211015054\"},\"PeriodicalIF\":3.0000,\"publicationDate\":\"2021-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/fe/ca/10.1177_20587384211015054.PMC8127738.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International Journal of Immunopathology and Pharmacology\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1177/20587384211015054\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"IMMUNOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of Immunopathology and Pharmacology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1177/20587384211015054","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"IMMUNOLOGY","Score":null,"Total":0}
引用次数: 0
摘要
简介SKF-96365被认为是受体介导的钙离子（Ca2+）进入的抑制剂。本研究旨在探讨 SKF-96365 对小鼠过敏性鼻炎（AR）的影响：方法：对 OVA 诱导的小鼠过敏性鼻炎进行鼻内注射 SKF-96365。采集小鼠血清和鼻腔灌洗液（NLF），用 ELISA 方法检测 IgE 和炎性细胞因子。对鼻腔灌洗液中的炎性细胞进行计数和分析。采集小鼠鼻黏膜组织，用于 HE 染色、免疫组织化学（IHC）染色和实时 PCR 检测：结果：SKF-96365对小鼠AR有治疗作用，可减轻AR小鼠的打喷嚏、鼻摩擦、IgE、炎性细胞因子、炎性细胞、TRPC6免疫标记以及TRPC6、STIM1和Orai1 mRNA水平：结论：SKF-96365能有效缓解小鼠AR的症状。结论：SKF-96365能有效缓解小鼠AR的症状，抑制TRPC6、STIM1和Orai1的活性，导致炎性细胞因子和炎性细胞的下调。
Therapeutic effects of SKF-96365 on murine allergic rhinitis induced by OVA.
Introduction: SKF-96365 is regarded as an inhibitor of receptor-mediated calcium ion (Ca2+) entry. The current study aimed to explore the effects of SKF-96365 on murine allergic rhinitis (AR).
Methods: Intranasal SKF-96365 administration was performed on OVA induced murine AR. Serum and nasal lavage fluid (NLF) from mice were harvested to assay IgE and inflammatory cytokines using ELISA method. Inflammatory cells were counted and analyzed in NLF. Nasal mucosa tissues were collected from mice and used for HE staining, immunohistochemistry (IHC) staining, and real-time PCR detection.
Results: SKF-96365 had therapeutic effects on murine AR manifesting attenuation of sneezing, nasal rubbing, IgE, inflammatory cytokines, inflammatory cells, TRPC6 immunolabeling, and TRPC6, STIM1 and Orai1 mRNA levels in AR mice.
Conclusion: SKF-96365 could effectively alleviate the symptoms of murine AR. SKF-96365 could suppress TRPC6, STIM1, and Orai1 activities, leading to the downregulation of inflammatory cytokines and inflammatory cells in murine AR.
期刊介绍：
International Journal of Immunopathology and Pharmacology is an Open Access peer-reviewed journal publishing original papers describing research in the fields of immunology, pathology and pharmacology. The intention is that the journal should reflect both the experimental and clinical aspects of immunology as well as advances in the understanding of the pathology and pharmacology of the immune system.
京公网安备 11010802042870号
京ICP备2023020795号-1
分享
求助内容：
应助结果提醒方式：
扫码关注我们
