Feedforward Control for Single Particle Tracking Synthetic Motion.

Single particle tracking (SPT) is a method to study the transport of biomolecules with nanometer resolution. Unfortunately, recent reports show that systematic errors in position localization and uncertainty in model parameter estimates limits the utility of these techniques in studying biological processes. There is a need for an experimental method with a known ground-truth that tests the total SPT system (sample, microscope, algorithm) on both localization and estimation of model parameters. Synthetic motion is a known ground-truth method that moves a particle along a trajectory. This trajectory is a realization of a Markovian stochastic process that represents models of biomolecular transport. Here we describe a platform for creating synthetic motion using common equipment and well-known, simple methods that can be easily adopted by the biophysics community. In this paper we describe the synthetic motion system and calibration to achieve nanometer accuracy and precision. Steady state input-output characteristics are analyzed with both line scans and grid scans. The resulting relationship is described by an affine transformation, which is inverted and used as a prefilter. Model inverse feed forward control is used to increase the system bandwidth. The system model was identified from frequency response function measurements using an integrated stepped-sine with coherent demodulation built into the FPGA controller. Zero magnitude error tracking controller method was used to invert non-minimum phase zeros to achieve a stable discrete time feed forward filter.