Bangjin Zheng , Chaoyang Huang , Jian Zhou , Lan Ye
{"title":"小鼠睾丸中一个新的Sox5转录物的鉴定","authors":"Bangjin Zheng , Chaoyang Huang , Jian Zhou , Lan Ye","doi":"10.1016/j.gep.2021.119197","DOIUrl":null,"url":null,"abstract":"<div><p>The transcription factor SOX5 is present in two distinct isoforms in both human and mouse, L-SOX5 and S-SOX5 (long and short isoforms of SOX5). Here, we identified and characterized a novel transcript of <em>Sox5</em> (S-<em>Sox5</em> variant) in mouse testis. eCLIP-based amplification of cDNA ends were performed to identify the potential <em>Sox5</em> mRNA variant. This novel transcript shares a high similarity with the previously reported S-<em>Sox5</em> in nucleotide sequence, but with a unique stretch of 5′UTR and an additional exon 9. Semi-quantitative PCR analysis revealed both S-<em>Sox5</em> variant and S-<em>Sox5</em> express specifically in mouse testis. Both transcripts increase significantly in mouse testis at postnatal day 21, when round spermatids appear. We further made a series of truncated <em>Sox5</em> constructs and tagged them with eGFP in HeLa cells. <em>In vitro</em> transfection assay identified the N-terminus and the DNA-binding HMG domain are required for the nuclear localization of SOX5. Our results provides a basis for the future study to investigate the biological function of SOX5 in spermatogenesis.</p></div>","PeriodicalId":55598,"journal":{"name":"Gene Expression Patterns","volume":null,"pages":null},"PeriodicalIF":1.0000,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.gep.2021.119197","citationCount":"3","resultStr":"{\"title\":\"Identification of a novel Sox5 transcript in mouse testis\",\"authors\":\"Bangjin Zheng , Chaoyang Huang , Jian Zhou , Lan Ye\",\"doi\":\"10.1016/j.gep.2021.119197\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>The transcription factor SOX5 is present in two distinct isoforms in both human and mouse, L-SOX5 and S-SOX5 (long and short isoforms of SOX5). Here, we identified and characterized a novel transcript of <em>Sox5</em> (S-<em>Sox5</em> variant) in mouse testis. eCLIP-based amplification of cDNA ends were performed to identify the potential <em>Sox5</em> mRNA variant. This novel transcript shares a high similarity with the previously reported S-<em>Sox5</em> in nucleotide sequence, but with a unique stretch of 5′UTR and an additional exon 9. Semi-quantitative PCR analysis revealed both S-<em>Sox5</em> variant and S-<em>Sox5</em> express specifically in mouse testis. Both transcripts increase significantly in mouse testis at postnatal day 21, when round spermatids appear. We further made a series of truncated <em>Sox5</em> constructs and tagged them with eGFP in HeLa cells. <em>In vitro</em> transfection assay identified the N-terminus and the DNA-binding HMG domain are required for the nuclear localization of SOX5. Our results provides a basis for the future study to investigate the biological function of SOX5 in spermatogenesis.</p></div>\",\"PeriodicalId\":55598,\"journal\":{\"name\":\"Gene Expression Patterns\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.0000,\"publicationDate\":\"2021-09-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/j.gep.2021.119197\",\"citationCount\":\"3\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Gene Expression Patterns\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1567133X21000326\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"DEVELOPMENTAL BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Gene Expression Patterns","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1567133X21000326","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"DEVELOPMENTAL BIOLOGY","Score":null,"Total":0}
Identification of a novel Sox5 transcript in mouse testis
The transcription factor SOX5 is present in two distinct isoforms in both human and mouse, L-SOX5 and S-SOX5 (long and short isoforms of SOX5). Here, we identified and characterized a novel transcript of Sox5 (S-Sox5 variant) in mouse testis. eCLIP-based amplification of cDNA ends were performed to identify the potential Sox5 mRNA variant. This novel transcript shares a high similarity with the previously reported S-Sox5 in nucleotide sequence, but with a unique stretch of 5′UTR and an additional exon 9. Semi-quantitative PCR analysis revealed both S-Sox5 variant and S-Sox5 express specifically in mouse testis. Both transcripts increase significantly in mouse testis at postnatal day 21, when round spermatids appear. We further made a series of truncated Sox5 constructs and tagged them with eGFP in HeLa cells. In vitro transfection assay identified the N-terminus and the DNA-binding HMG domain are required for the nuclear localization of SOX5. Our results provides a basis for the future study to investigate the biological function of SOX5 in spermatogenesis.
期刊介绍:
Gene Expression Patterns is devoted to the rapid publication of high quality studies of gene expression in development. Studies using cell culture are also suitable if clearly relevant to development, e.g., analysis of key regulatory genes or of gene sets in the maintenance or differentiation of stem cells. Key areas of interest include:
-In-situ studies such as expression patterns of important or interesting genes at all levels, including transcription and protein expression
-Temporal studies of large gene sets during development
-Transgenic studies to study cell lineage in tissue formation