巯基氧化还原系统能否作为合成生物学应用的一部分?

IF 5.2 2区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Redox Report Pub Date : 2021-12-01 DOI:10.1080/13510002.2021.1966183
Ché S Pillay, Nolyn John
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引用次数: 0

摘要

目标:合成生物学已从分子生物学和工程方法中脱颖而出,旨在开发新的、受生物学启发的系统,用于从生物计算到药物生产的工业和基础研究应用。令人惊讶的是,氧还蛋白(硫氧还蛋白、戊二氧还蛋白和过氧铁氧还蛋白)和其他基于硫醇的氧化还原系统尚未在许多合成生物学应用中广泛使用。方法:我们综述了基于硫醇的氧化还原系统以及使用硫醇依赖部分的合成生物学应用的发展。结果:开发促进细胞质二硫键、生物计算和治疗肠道疾病的电路是使用硫醇基部件的应用之一。我们提出,基因编码的氧化还原传感器、硫醇基生物材料和细胞内过氧化氢发生器也可能是合成生物学应用的有价值的组成部分。讨论:基于硫醇的系统在细胞氧化还原代谢、抗氧化防御和信号传导中发挥着多种作用,因此可以为合成生物学应用提供大量多样的组件、零件和设备。然而,限制氧还蛋白系统用于合成生物学应用的因素包括基于硫醇的组分的正交性、表征基于硫醇的系统的方法的局限性以及对这些系统的设计原理的不完全理解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Can thiol-based redox systems be utilized as parts for synthetic biology applications?

Objectives: Synthetic biology has emerged from molecular biology and engineering approaches and aims to develop novel, biologically-inspired systems for industrial and basic research applications ranging from biocomputing to drug production. Surprisingly, redoxin (thioredoxin, glutaredoxin, peroxiredoxin) and other thiol-based redox systems have not been widely utilized in many of these synthetic biology applications.

Methods: We reviewed thiol-based redox systems and the development of synthetic biology applications that have used thiol-dependent parts.

Results: The development of circuits to facilitate cytoplasmic disulfide bonding, biocomputing and the treatment of intestinal bowel disease are amongst the applications that have used thiol-based parts. We propose that genetically encoded redox sensors, thiol-based biomaterials and intracellular hydrogen peroxide generators may also be valuable components for synthetic biology applications.

Discussion: Thiol-based systems play multiple roles in cellular redox metabolism, antioxidant defense and signaling and could therefore offer a vast and diverse portfolio of components, parts and devices for synthetic biology applications. However, factors limiting the adoption of redoxin systems for synthetic biology applications include the orthogonality of thiol-based components, limitations in the methods to characterize thiol-based systems and an incomplete understanding of the design principles of these systems.

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来源期刊
Redox Report
Redox Report 生物-生化与分子生物学
CiteScore
6.10
自引率
0.00%
发文量
28
审稿时长
>12 weeks
期刊介绍: Redox Report is a multidisciplinary peer-reviewed open access journal focusing on the role of free radicals, oxidative stress, activated oxygen, perioxidative and redox processes, primarily in the human environment and human pathology. Relevant papers on the animal and plant environment, biology and pathology will also be included. While emphasis is placed upon methodological and intellectual advances underpinned by new data, the journal offers scope for review, hypotheses, critiques and other forms of discussion.
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