重组人成纤维细胞生长因子2治疗三度烧伤小鼠。

IF 1.8 4区 生物学 Q4 CELL BIOLOGY Growth factors Pub Date : 2020-12-01 Epub Date: 2021-08-20 DOI:10.1080/08977194.2021.1967342
Thu-Minh Tran-Nguyen, Khanh-Thien Le, Le-Giang Thi Nguyen, Thanh-Loan Thi Tran, Phuong-Cac Hoang-Thai, Thuoc Linh Tran, Sik-Loo Tan, Hieu Tran-Van
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引用次数: 3

摘要

成纤维细胞生长因子2 (FGF-2)是一种多功能蛋白,在伤口愈合、组织修复和再生中起重要作用。这种治疗性蛋白被广泛用于烧伤治疗,因为它可以刺激细胞增殖和分化,血管生成和细胞外基质重塑。在这项研究中,我们开发了一种简单的方法,使用控制加热的黄铜棒来创建均匀的三度烧伤小鼠模型,并评估了重组人FGF-2 (rhFGF-2)的治疗效果。结果显示,创面面积为0.83±0.05 cm2,创面深度为573.42±147.82 μm。与磷酸盐缓冲盐水(PBS)处理组相比,用rhFGF-2处理的小鼠伤口愈合、肉芽组织形成、血管生成和再上皮化的率更高。总之,我们实验室制造的rhFGF-2可能是一种潜在的治疗性蛋白,用于烧伤治疗,也可能是一种生物等效药物,用于其他商业应用。
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Third-degree burn mouse treatment using recombinant human fibroblast growth factor 2.

Fibroblast growth factor 2 (FGF-2) is a multifunctional protein that has major roles in wound healing, tissue repair, and regeneration. This therapeutic protein is widely used for burn treatment because it can stimulate cell proliferation and differentiation, angiogenesis, and extracellular matrix remodeling. In this study, we developed a simple method using a controlled heated brass rod to create a homogenous third-degree burn murine model and evaluated the treatment using recombinant human FGF-2 (rhFGF-2). The results indicated that the wound area was 0.83 ± 0.05 cm2 and wound depth was 573.42 ± 147.82 μm. Mice treated with rhFGF-2 showed higher rates of wound closure, granulation tissue formation, angiogenesis, and re-epithelialization than that of phosphate-buffered saline (PBS)-treated group. In conclusion, our lab-made rhFGF-2 could be a potentially therapeutic protein for burn treatment as well as a bioequivalent drug for other commercial applications using FGF-2.

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来源期刊
Growth factors
Growth factors 生物-内分泌学与代谢
CiteScore
2.60
自引率
0.00%
发文量
20
审稿时长
>12 weeks
期刊介绍: Growth Factors is an international and interdisciplinary vehicle publishing new knowledge and findings on the regulators of cell proliferation, differentiation and survival. The Journal will publish research papers, short communications and reviews on current developments in cell biology, biochemistry, physiology or pharmacology of growth factors, cytokines or hormones which improve our understanding of biology or medicine. Among the various fields of study topics of particular interest include: •Stem cell biology •Growth factor physiology •Structure-activity relationships •Drug development studies •Clinical applications
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