金刚石纳米膜对肝癌细胞增殖和代谢的影响。

IF 4.9 Q2 NANOSCIENCE & NANOTECHNOLOGY Nanotechnology, Science and Applications Pub Date : 2021-08-28 eCollection Date: 2021-01-01 DOI:10.2147/NSA.S322766
Malwina Sosnowska, Marta Kutwin, Barbara Strojny, Mateusz Wierzbicki, Dominik Cysewski, Jarosław Szczepaniak, Mateusz Ficek, Piotr Koczoń, Sławomir Jaworski, André Chwalibog, Ewa Sawosz
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引用次数: 2

摘要

目的:肝细胞癌的手术切除可能与肝残余体积变性引起的复发有关。目的是评估使用由金刚石纳米颗粒胶体(nfND)制成的生物相容性纳米膜填充肿瘤切除后一侧的可能性,并优化其与增殖的肝细胞的接触,最大限度地减少其癌变。方法:采用HepG2、C3A肝癌细胞和HS-5非癌细胞。覆盖在细胞培养板上的金刚石纳米颗粒的水性胶体被用来制造纳米膜。通过原子力显微镜测量所得纳米膜的粗糙度。XTT和BrdU检测线粒体活性和细胞增殖。用细胞形态学和划痕试验来评价细胞的侵袭性。流式细胞术测定细胞周期内的细胞数量。质谱法测定蛋白表达。结果:与标准板相比,nfND产生了粗糙度增加和暴露氧基团的表面。所有细胞系都倾向于在纳米膜上沉淀,但癌细胞形成更宽松的簇。表面相容性随细胞类型的不同而降低,其顺序为C3A >HepG2 >HS-5。肿瘤细胞系侵袭减少,对C3A细胞系影响最大,增殖减少,G2/M细胞数量增加。表达改变的蛋白以膜蛋白和核蛋白为主。结论:体外实验证实了nfND对C3A肝癌细胞的抗增殖作用。与此同时,由于C3A与HepG2细胞的蛋白质合成反应存在差异,因此需要个性化潜在治疗。我们记录了nfND是一个信号来源,能够使许多细胞内蛋白的表达正常化,这些蛋白参与了向非癌细胞的转化。
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Diamond Nanofilm Normalizes Proliferation and Metabolism in Liver Cancer Cells.

Purpose: Surgical resection of hepatocellular carcinoma can be associated with recurrence resulting from the degeneration of residual volume of the liver. The objective was to assess the possibility of using a biocompatible nanofilm, made of a colloid of diamond nanoparticles (nfND), to fill the side after tumour resection and optimize its contact with proliferating liver cells, minimizing their cancerous transformation.

Methods: HepG2 and C3A liver cancer cells and HS-5 non-cancer cells were used. An aqueous colloid of diamond nanoparticles, which covered the cell culture plate, was used to create the nanofilm. The roughness of the resulting nanofilm was measured by atomic force microscopy. Mitochondrial activity and cell proliferation were measured by XTT and BrdU assays. Cell morphology and a scratch test were used to evaluate the invasiveness of cells. Flow cytometry determined the number of cells within the cell cycle. Protein expression in was measured by mass spectrometry.

Results: The nfND created a surface with increased roughness and exposed oxygen groups compared with a standard plate. All cell lines were prone to settling on the nanofilm, but cancer cells formed more relaxed clusters. The surface compatibility was dependent on the cell type and decreased in the order C3A >HepG2 >HS-5. The invasion was reduced in cancer lines with the greatest effect on the C3A line, reducing proliferation and increasing the G2/M cell population. Among the proteins with altered expression, membrane and nuclear proteins dominated.

Conclusion: In vitro studies demonstrated the antiproliferative properties of nfND against C3A liver cancer cells. At the same time, the need to personalize potential therapy was indicated due to the differential protein synthetic responses in C3A vs HepG2 cells. We documented that nfND is a source of signals capable of normalizing the expression of many intracellular proteins involved in the transformation to non-cancerous cells.

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来源期刊
Nanotechnology, Science and Applications
Nanotechnology, Science and Applications NANOSCIENCE & NANOTECHNOLOGY-
CiteScore
11.70
自引率
0.00%
发文量
3
审稿时长
16 weeks
期刊介绍: Nanotechnology, Science and Applications is an international, peer-reviewed, Open Access journal that focuses on the science of nanotechnology in a wide range of industrial and academic applications. The journal is characterized by the rapid reporting of reviews, original research, and application studies across all sectors, including engineering, optics, bio-medicine, cosmetics, textiles, resource sustainability and science. Applied research into nano-materials, particles, nano-structures and fabrication, diagnostics and analytics, drug delivery and toxicology constitute the primary direction of the journal.
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