在两种小鼠哮喘模型中,双室容积描记术与振荡法检测基线气流阻塞。

IF 1.5 4区 医学 Q3 RESPIRATORY SYSTEM Experimental Lung Research Pub Date : 2021-10-01 Epub Date: 2021-09-20 DOI:10.1080/01902148.2021.1979693
Magali Boucher, Cyndi Henry, Fatemeh Khadangi, Alexis Dufour-Mailhot, Ynuk Bossé
{"title":"在两种小鼠哮喘模型中,双室容积描记术与振荡法检测基线气流阻塞。","authors":"Magali Boucher,&nbsp;Cyndi Henry,&nbsp;Fatemeh Khadangi,&nbsp;Alexis Dufour-Mailhot,&nbsp;Ynuk Bossé","doi":"10.1080/01902148.2021.1979693","DOIUrl":null,"url":null,"abstract":"<p><strong>Aim of the study: </strong>The current gold standard to assess respiratory mechanics in mice is oscillometry, a technique from which several readouts of the respiratory system can be deduced, such as resistance and elastance. However, these readouts are often not altered in mouse models of asthma. This is in stark contrast with humans, where asthma is generally associated with alterations when assessed by either oscillometry or other techniques. In the present study, we have used double-chamber plethysmography (DCP) to evaluate the breathing pattern and the degree of airflow obstruction in a mouse model of asthma.</p><p><strong>Materials and methods: </strong>Female C57BL/6 and BALB/c mice were studied at day 1 using DCP, as well as at day 11 using both DCP and oscillometry following a once-daily exposure to either house-dust mite (HDM) or saline for 10 consecutive days.</p><p><strong>Results: </strong>All DCP readouts used to describe either the breathing pattern (e.g., tidal volume and breathing frequency) or the degree of airflow obstruction (e.g., specific airway resistance) were different between mouse strains at day 1. Most of these strain differences persisted at day 11. Most oscillometric readouts (e.g., respiratory system resistance and elastance) were also different between strains. Changes caused by HDM were obvious with DCP, including decreases in tidal volume, minute ventilation, inspiratory time and mid-tidal expiratory flow and an increase in specific airway resistance. HDM also caused some strain specific alterations in breathing pattern, including increases in expiratory time and end inspiratory pause, which were only observed in C57BL/6 mice. Oscillometry also detected a small but significant increase in tissue elastance in HDM <i>versus</i> saline-exposed mice.</p><p><strong>Conclusions: </strong>DCP successfully identified differences between C57BL/6 and BALB/c mice, as well as alterations in mice from both strains exposed to HDM. We conclude that, depending on the study purpose, DCP may sometimes outweigh oscillometry.</p>","PeriodicalId":12206,"journal":{"name":"Experimental Lung Research","volume":"47 8","pages":"390-401"},"PeriodicalIF":1.5000,"publicationDate":"2021-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"5","resultStr":"{\"title\":\"Double-chamber plethysmography <i>versus</i> oscillometry to detect baseline airflow obstruction in a model of asthma in two mouse strains.\",\"authors\":\"Magali Boucher,&nbsp;Cyndi Henry,&nbsp;Fatemeh Khadangi,&nbsp;Alexis Dufour-Mailhot,&nbsp;Ynuk Bossé\",\"doi\":\"10.1080/01902148.2021.1979693\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Aim of the study: </strong>The current gold standard to assess respiratory mechanics in mice is oscillometry, a technique from which several readouts of the respiratory system can be deduced, such as resistance and elastance. However, these readouts are often not altered in mouse models of asthma. This is in stark contrast with humans, where asthma is generally associated with alterations when assessed by either oscillometry or other techniques. In the present study, we have used double-chamber plethysmography (DCP) to evaluate the breathing pattern and the degree of airflow obstruction in a mouse model of asthma.</p><p><strong>Materials and methods: </strong>Female C57BL/6 and BALB/c mice were studied at day 1 using DCP, as well as at day 11 using both DCP and oscillometry following a once-daily exposure to either house-dust mite (HDM) or saline for 10 consecutive days.</p><p><strong>Results: </strong>All DCP readouts used to describe either the breathing pattern (e.g., tidal volume and breathing frequency) or the degree of airflow obstruction (e.g., specific airway resistance) were different between mouse strains at day 1. Most of these strain differences persisted at day 11. Most oscillometric readouts (e.g., respiratory system resistance and elastance) were also different between strains. Changes caused by HDM were obvious with DCP, including decreases in tidal volume, minute ventilation, inspiratory time and mid-tidal expiratory flow and an increase in specific airway resistance. HDM also caused some strain specific alterations in breathing pattern, including increases in expiratory time and end inspiratory pause, which were only observed in C57BL/6 mice. Oscillometry also detected a small but significant increase in tissue elastance in HDM <i>versus</i> saline-exposed mice.</p><p><strong>Conclusions: </strong>DCP successfully identified differences between C57BL/6 and BALB/c mice, as well as alterations in mice from both strains exposed to HDM. We conclude that, depending on the study purpose, DCP may sometimes outweigh oscillometry.</p>\",\"PeriodicalId\":12206,\"journal\":{\"name\":\"Experimental Lung Research\",\"volume\":\"47 8\",\"pages\":\"390-401\"},\"PeriodicalIF\":1.5000,\"publicationDate\":\"2021-10-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"5\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Experimental Lung Research\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1080/01902148.2021.1979693\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2021/9/20 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q3\",\"JCRName\":\"RESPIRATORY SYSTEM\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Experimental Lung Research","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1080/01902148.2021.1979693","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2021/9/20 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"RESPIRATORY SYSTEM","Score":null,"Total":0}
引用次数: 5

摘要

研究目的:目前评估小鼠呼吸力学的金标准是振荡测量法,这种技术可以推断出呼吸系统的几个读数,比如阻力和弹性。然而,在哮喘小鼠模型中,这些读数通常不会改变。这与人类形成鲜明对比,在人类中,通过振荡测量法或其他技术评估时,哮喘通常与改变有关。在本研究中,我们采用双腔容积描记术(DCP)评估哮喘小鼠模型的呼吸方式和气流阻塞程度。材料和方法:雌性C57BL/6和BALB/c小鼠在第1天使用DCP,在第11天使用DCP和振荡测定法,连续10天每天一次暴露于屋尘螨(HDM)或生理盐水中。结果:所有用于描述呼吸方式(如潮气量和呼吸频率)或气流阻塞程度(如特定气道阻力)的DCP读数在第1天的小鼠品系之间都是不同的。这些差异大部分在第11天仍然存在。大多数振荡读数(例如,呼吸系统阻力和弹性)在菌株之间也不同。DCP患者HDM引起的变化明显,包括潮气量、分钟通气量、吸气时间和潮中呼气流量的减少以及比气道阻力的增加。HDM还引起了一些菌株特有的呼吸模式改变,包括呼气时间和吸气末暂停的增加,这仅在C57BL/6小鼠中观察到。振荡测量法还检测到HDM与盐暴露小鼠的组织弹性虽小但显著增加。结论:DCP成功鉴定了C57BL/6和BALB/c小鼠之间的差异,以及暴露于HDM的两株小鼠的变化。我们得出结论,根据研究目的,DCP有时可能超过振荡测量。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
Double-chamber plethysmography versus oscillometry to detect baseline airflow obstruction in a model of asthma in two mouse strains.

Aim of the study: The current gold standard to assess respiratory mechanics in mice is oscillometry, a technique from which several readouts of the respiratory system can be deduced, such as resistance and elastance. However, these readouts are often not altered in mouse models of asthma. This is in stark contrast with humans, where asthma is generally associated with alterations when assessed by either oscillometry or other techniques. In the present study, we have used double-chamber plethysmography (DCP) to evaluate the breathing pattern and the degree of airflow obstruction in a mouse model of asthma.

Materials and methods: Female C57BL/6 and BALB/c mice were studied at day 1 using DCP, as well as at day 11 using both DCP and oscillometry following a once-daily exposure to either house-dust mite (HDM) or saline for 10 consecutive days.

Results: All DCP readouts used to describe either the breathing pattern (e.g., tidal volume and breathing frequency) or the degree of airflow obstruction (e.g., specific airway resistance) were different between mouse strains at day 1. Most of these strain differences persisted at day 11. Most oscillometric readouts (e.g., respiratory system resistance and elastance) were also different between strains. Changes caused by HDM were obvious with DCP, including decreases in tidal volume, minute ventilation, inspiratory time and mid-tidal expiratory flow and an increase in specific airway resistance. HDM also caused some strain specific alterations in breathing pattern, including increases in expiratory time and end inspiratory pause, which were only observed in C57BL/6 mice. Oscillometry also detected a small but significant increase in tissue elastance in HDM versus saline-exposed mice.

Conclusions: DCP successfully identified differences between C57BL/6 and BALB/c mice, as well as alterations in mice from both strains exposed to HDM. We conclude that, depending on the study purpose, DCP may sometimes outweigh oscillometry.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Experimental Lung Research
Experimental Lung Research 医学-呼吸系统
CiteScore
3.80
自引率
0.00%
发文量
23
审稿时长
2 months
期刊介绍: Experimental Lung Research publishes original articles in all fields of respiratory tract anatomy, biology, developmental biology, toxicology, and pathology. Emphasis is placed on investigations concerned with molecular, biochemical, and cellular mechanisms of normal function, pathogenesis, and responses to injury. The journal publishes reports on important methodological advances on new experimental modes. Also published are invited reviews on important and timely research advances, as well as proceedings of specialized symposia. Authors can choose to publish gold open access in this journal.
期刊最新文献
Exosomes derived from hypoxic alveolar epithelial cells promote the phenotypic transformation of pulmonary artery smooth muscle cells via the Rap1 pathway Treatment with inhaled aerosolised ethanol reduces viral load and potentiates macrophage responses in an established influenza mouse model Inhibition of GBP5 activates autophagy to alleviate inflammatory response in LPS-induced lung injury in mice Differential changes in expression of inflammatory mRNA and protein after oleic acid-induced acute lung injury CLCA1 exacerbates lung inflammation via p38 MAPK pathway in acute respiratory distress syndrome
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1