4-己基间苯二酚对Saos-2细胞NAD+水平和SIRT活性的影响。

IF 2 Q2 DENTISTRY, ORAL SURGERY & MEDICINE Maxillofacial Plastic and Reconstructive Surgery Pub Date : 2021-11-01 DOI:10.1186/s40902-021-00326-2

In-Song Lee, Jun-Ho Chang, Dae-Won Kim, Seong-Gon Kim, Tae-Woo Kim

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引用次数: 3

摘要

背景:4-己基间苯二酚(4HR)已被证明具有与白藜芦醇相似的抗氧化活性。当白藜芦醇增加sirtuin (SIRT)活性时，4HR的行为可能与白藜芦醇相似。方法:在给药4HR (1 ~ 100 μM)后，检测SIRT1、SIRT3、SIRT6的表达水平。由于NAD+是SIRT的底物，其水平与SIRT活性也进行了研究。结果:SIRT3 (100 μM, 24 h)和SIRT6 (1 ~ 100 μM, 24 h, 10 μM, 8 h)的表达量显著高于对照组(p < 0.05)。与对照组相比，Pan-SIRT活性和NAD+水平显著升高(p < 0.05;结论:4HR可提高Saos-2细胞SIRT活性和NAD+水平。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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The effect of 4-hexylresorinol administration on NAD+ level and SIRT activity in Saos-2 cells.

Background: 4-hexylresorcinol (4HR) has been shown to have anti-oxidant activity similar to that of resveratrol. As resveratrol increases sirtuin (SIRT) activity, 4HR might behave similarly to resveratrol.

Method: In this study, the expression levels of SIRT1, SIRT3, and SIRT6 were evaluated after 4HR administration (1-100 μM). As NAD+ is a substrate for SIRTs, its levels with SIRT activity were also studied.

Results: In the results, SIRT3 (100 μM at 24 h) and SIRT6 (1-100 μM at 24 h and 10 μM at 8 h) were shown to have significantly higher expression levels compared to untreated control (p < 0.05). Pan-SIRT activity and the NAD+ level was significantly increased compared to that of the untreated control (p < 0.05; 10 and 100 μM at 24 h).

Conclusion: 4HR administration increased SIRT activity and the NAD+ level in Saos-2 cells.

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