不同严重程度糖尿病肾病中循环 TGFβ 调控 miRNA 的相互作用

IF 2.5 4区医学 Q3 ENDOCRINOLOGY & METABOLISM Archives of Physiology and Biochemistry Pub Date : 2024-06-01 Epub Date: 2022-02-11 DOI:10.1080/13813455.2022.2034884

Huiwen Ren, Ying Shao, Xiaoyu Ma, Li An, Yu Liu, Qiuyue Wang

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引用次数: 0

摘要

目的：探讨TGFβ调控微RNA（miRNA）与不同严重程度糖尿病肾病（DKD）的相互作用：方法：根据不同的 UACR（30 和 300 mg/g），纳入 436 名受试者，培养高糖诱导的 RMCs。采用实时 PCR、ELISA 和自动生化分析技术检测血清和 RMC 中的 miRNA、TGFβ1 及其他生化指标。通过生物信息学方法对 miRNA 的靶基因进行了预测和可视化：结果：T2DM 患者的 HbA1c、TGFβ1、miR-217 和 miR-224 随 UACR 的升高而升高，而 miR-192 和 miR-216a 则随 UACR 的降低而降低。Ln UACR 与 HbA1c、TGFβ1、miR-217 和 miR-224 呈正相关，而与 miR-192 和 miR-216a 呈负相关。高糖和 TGFβ1 影响了 miRNA，而这些 miRNA 又相互影响。miRNA的靶基因主要围绕PTEN、PI3K/Akt和MAPK信号通路：结论：TGFβ调控miRNA与不同严重程度的DKD有潜在的相互作用，通过PTEN、PI3K/Akt和MAPK通路调控纤维化。

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Interaction of circulating TGFβ regulatory miRNAs in different severity of diabetic kidney disease.

Aims: To explore the interaction of TGFβ regulatory microRNAs (miRNAs) with different severities of diabetic kidney disease (DKD).

Methods: According to different UACR (30 and 300 mg/g), 436 subjects were included, and high glucose induced RMCs were cultured. Real-time PCR, ELISA, and automatic biochemical analysis were used to measure miRNAs, TGFβ1, and other biochemical indicators in serum and RMCs. Target genes of miRNA were predicted and visualised by bioinformatics.

Results: HbA1c, TGFβ1, miR-217, and miR-224 in T2DM patients increased with UACR, while miR-192 and miR-216a decreased. Ln UACR was positively correlated with HbA1c, TGFβ1, miR-217, and miR-224, and negatively correlated with miR-192 and miR-216a. High glucose and TGFβ1 affected miRNAs and these miRNAs affected each other. The miRNA target genes mainly revolve around PTEN, PI3K/Akt, and MAPK signalling pathways.

Conclusion: TGFβ regulatory miRNAs and different severity of DKD have a potential interaction regulating fibrosis through PTEN, PI3K/Akt, and MAPK pathways.

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来源期刊

Archives of Physiology and Biochemistry ENDOCRINOLOGY & METABOLISM-PHYSIOLOGY

CiteScore

6.90

自引率

3.30%

发文量

期刊介绍： Archives of Physiology and Biochemistry: The Journal of Metabolic Diseases is an international peer-reviewed journal which has been relaunched to meet the increasing demand for integrated publication on molecular, biochemical and cellular aspects of metabolic diseases, as well as clinical and therapeutic strategies for their treatment. It publishes full-length original articles, rapid papers, reviews and mini-reviews on selected topics. It is the overall goal of the journal to disseminate novel approaches to an improved understanding of major metabolic disorders. The scope encompasses all topics related to the molecular and cellular pathophysiology of metabolic diseases like obesity, type 2 diabetes and the metabolic syndrome, and their associated complications. Clinical studies are considered as an integral part of the Journal and should be related to one of the following topics: -Dysregulation of hormone receptors and signal transduction -Contribution of gene variants and gene regulatory processes -Impairment of intermediary metabolism at the cellular level -Secretion and metabolism of peptides and other factors that mediate cellular crosstalk -Therapeutic strategies for managing metabolic diseases Special issues dedicated to topics in the field will be published regularly.