天然成分实验动物饲料中产气荚膜梭菌的产毒特性分析。

IF 1.3 4区 农林科学 Q2 VETERINARY SCIENCES Comparative medicine Pub Date : 2022-02-01 Epub Date: 2022-02-11 DOI:10.30802/AALAS-CM-22-000013
Michael D Johnston, Tanya E Whiteside, Michelle E Allen, David M Kurtz
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引用次数: 2

摘要

产气荚膜梭菌是一种厌氧、革兰氏阳性、孢子形成的细菌,普遍存在于各种自然环境中,包括人类和动物的胃肠道。产气荚膜梭菌是一种机会性致病菌,能够产生至少20种不同组合的毒素。根据α、β、epsilon、iota、肠毒素和netB 6种毒素的存在与否,目前将产气荚膜荚膜杆菌菌株分为A、B、C、D、E、F和G 7种毒素型。每种毒素型都与特定的组织毒性和肠道疾病有关。在实验动物中有由产气荚膜原梭菌引起的自发性肠炎的报道;然而,细菌的来源尚不清楚。国家环境卫生科学研究所(NIEHS)的质量保证实验室(QAL)定期对进料的动物饲料进行需氧、肠道病原体的筛查,如沙门氏菌和大肠杆菌。最近,QAL纳入了对进料动物饲料的厌氧筛选。迄今为止,该实验室已从23批天然成分实验动物饲料中分离出多种梭菌,包括产气荚膜梭菌。从实验动物饲料中分离出产气荚膜荚膜杆菌的文献尚未见报道。因此,我们使用PCR技术对分离的产气荚膜荚膜梭菌菌株进行了毒素谱筛选,以确定实验动物饮食中存在哪些毒素类型。结果表明,从实验动物饲料中分离到的产气荚膜荚膜荚膜菌大部分为A型毒素,大部分菌株还含有θ毒素。其中两株产气荚膜荚膜杆菌也含有β毒素。我们的研究结果表明,产气荚膜荚膜杆菌存在于实验动物的非无菌天然成分饲料中,可能是这种机会性病原体的来源。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Toxigenic Profile of Clostridium perfringens Strains Isolated from Natural Ingredient Laboratory Animal Diets.

Clostridium perfringens is an anaerobic, gram-positive, spore-forming bacterium that ubiquitously inhabits a wide variety of natural environments including the gastrointestinal tract of humans and animals. C. perfringens is an opportunistic enteropathogen capable of producing at least 20 different toxins in various combinations. Strains of C. perfringens are currently categorized into 7 toxinotypes (A, B, C, D, E, F, and G) based on the presence or absence of 6 typing-toxins (α, β, epsilon, iota, enterotoxin, and netB). Each toxinotype is associated with specific histotoxic and enteric diseases. Spontaneous enteritis due to C. perfringens has been reported in laboratory animals; however, the source of the bacteria was unknown. The Quality Assurance Laboratory (QAL) at the National Institute of Environmental Health Sciences (NIEHS) routinely screens incoming animal feeds for aerobic, enteric pathogens, such as Salmonella spp. and E. coli. Recently, QAL incorporated anaerobic screening of incoming animal feeds. To date, the lab has isolated numerous Clostridium species, including C. perfringens, from 23 lots of natural ingredient laboratory animal diets. Published reports of C. perfringens isolation from laboratory animal feeds could not be found in the literature. Therefore, we performed a toxin profile screen of our isolated strains of C. perfringens using PCR to determine which toxinotypes were present in the laboratory animal diets. Our results showed that most C. perfringens strains we isolated from the laboratory animal feed were toxinotype A with most strains also possessing the theta toxin. Two of the C. perfringens strains also possessed the β toxin. Our results demonstrated the presence of C. perfringens in nonsterile, natural ingredient feeds for laboratory animals which could serve as a source of this opportunistic pathogen.

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来源期刊
Comparative medicine
Comparative medicine 医学-动物学
CiteScore
1.90
自引率
0.00%
发文量
71
审稿时长
6-12 weeks
期刊介绍: Comparative Medicine (CM), an international journal of comparative and experimental medicine, is the leading English-language publication in the field and is ranked by the Science Citation Index in the upper third of all scientific journals. The mission of CM is to disseminate high-quality, peer-reviewed information that expands biomedical knowledge and promotes human and animal health through the study of laboratory animal disease, animal models of disease, and basic biologic mechanisms related to disease in people and animals.
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