牙本质矿物质是否随解剖位置、显微位置和患者年龄而改变?

IF 3.5 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Journal of Structural Biology: X Pub Date : 2022-01-01 DOI:10.1016/j.yjsbx.2022.100060
Arosha T. Weerakoon , Crystal Cooper , Ian A. Meyers , Nicholas Condon , Christopher Sexton , David Thomson , Pauline J. Ford , Anne L. Symons
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引用次数: 4

摘要

目的探讨患者年龄(年轻或成熟)、解剖位置(浅/深、中央/外周)和显微部位(管间/管周)对牙本质矿物质密度、分布和组成的影响。方法青年(19 ~ 20岁,N = 4)和成年(54 ~ 77岁,N = 4)拔牙后牙分别做浅、深切片。采用背散射电子(BSE)显微照片、能量色散x射线能谱和综合矿物分析,在扫描电子显微镜下研究了牙本质表面元素组成。使用机器学习进行定性比较和定量测量来分析疯牛病图像。定量结果比较使用分位数或线性回归模型与自举,以解释每个样本的多个措施。随后,使用氙等离子体聚焦离子束扫描电子显微镜(Xe PFIB-SEM)对大面积(100µm)的横截面进行铣削,利用高分辨率二次电子显微镜观察牙本质小管的形貌。结果随着年龄的增长,牙本质矿物组成保持稳定,但密度随解剖位置和显微部位的变化而变化。镜下,副小管从主小管腔扩散到管间牙本质(ITD)。在管腔内,矿物质沉积物在幼时形成钙球石,最终在成熟的小管和分枝中结合。成熟牙本质的矿物阻塞增加了整体牙本质过渡段密度,以反映小管周围牙本质(PTD)浸润。显微照片上观察到的过渡段在年龄和观察平面上保持一致,表明小管沉积影响整体牙本质密度。矿物密度取决于PTD与ITD的相对分布,这种分布随解剖位置的不同而变化。粘接剂材料在牙齿内部和不同年龄组之间的相互作用可能不同。
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Does dentine mineral change with anatomical location, microscopic site and patient age?

Objective

To determine the effect of patient age (young or mature), anatomical location (shallow/deep and central/peripheral) and microscopic site (intertubular/peritubular) on dentine mineral density, distribution and composition.

Methods

Extracted posterior teeth from young (aged 19–20 years, N = 4) and mature (aged 54–77 years, N = 4) subjects were prepared to shallow and deep slices. The dentine surface elemental composition was investigated in a SEM using Backscattered Electron (BSE) micrographs, Energy Dispersive X-ray Spectroscopy, and Integrated Mineral Analysis. Qualitative comparisons and quantitative measures using machine learning were used to analyse the BSE images. Quantitative outcomes were compared using quantile or linear regression models with bootstrapping to account for the multiple measures per sample. Subsequently, a Xenon Plasma Focussed Ion Beam Scanning Electron Microscopy (Xe PFIB-SEM) was used to mill large area (100 µm) cross-sections to investigate morphology through the dentine tubules using high resolution secondary electron micrographs.

Results

With age, dentine mineral composition remains stable, but density changes with anatomical location and microscopic site. Microscopically, accessory tubules spread into intertubular dentine (ITD) from the main tubule lumens. Within the lumens, mineral deposits form calcospherites in the young that eventually coalesce in mature tubules and branches. The mineral occlusion in mature dentine increases overall ITD density to reflect peritubular dentine (PTD) infiltrate. The ITD observed in micrographs remained consistent for age and observation plane to suggest tubule deposition affects overall dentine density. Mineral density depends on the relative distribution of PTD to ITD that varies with anatomical location.

Significance

Adhesive materials may interact differently within a tooth as well as in different age groups.

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来源期刊
Journal of Structural Biology: X
Journal of Structural Biology: X Biochemistry, Genetics and Molecular Biology-Structural Biology
CiteScore
6.50
自引率
0.00%
发文量
20
审稿时长
62 days
期刊最新文献
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