{"title":"增强溃疡性结肠炎患者直肠CD45RA-CD62L+中枢记忆T,降低CD3+CD56+自然杀伤T淋巴细胞亚群。","authors":"Masaya Iwamuro, Takahide Takahashi, Natsuki Watanabe, Takehiro Tanaka, Toshihiro Inokuchi, Sakiko Hiraoka, Fumio Otsuka, Hiroyuki Okada","doi":"10.1177/20587384211051982","DOIUrl":null,"url":null,"abstract":"<p><strong>Objectives: </strong>To investigate the distinctive features of lymphocytes promoting inflammation in ulcerative colitis.</p><p><strong>Methods: </strong>We performed flow cytometric analysis of peripheral blood mononuclear cells (PBMCs) and colorectal mucosa lymphocytes in ulcerative colitis patients (<i>n</i> = 13) and control patients (<i>n</i> = 5).</p><p><strong>Results: </strong>CD62L<sup>+</sup>/CD3<sup>+</sup>CD4<sup>+</sup> (35.7 ± 14.0% vs. 19.9 ± 6.4%) and CD62L<sup>+</sup>/CD3<sup>+</sup>CD4<sup>-</sup> cells (17.1 ± 17.4% vs. 2.4 ± 3.9%) were higher in the rectum of ulcerative colitis patients than in control patients. Subpopulation analysis revealed that CD45RA<sup>-</sup>CD62L<sup>+</sup>/CD3<sup>+</sup>CD4<sup>+</sup>, that is, central memory T cell fraction in CD4<sup>+</sup> T cells, was significantly increased in the rectum of ulcerative colitis, compared to that in control patients (23.3 ± 10.5% vs. 8.2 ± 4.0%). Comparison of rectum and colon samples in ulcerative colitis patients indicated that CD56<sup>+</sup>/CD3<sup>+</sup> was decreased in the rectum compared to that in the colon (11.3 ± 12.5% vs. 21.3 ± 16.5%). The ratio of CD56<sup>+</sup>/CD3<sup>+</sup> was also decreased in the rectum of active ulcerative colitis patients compared to that in ulcerative colitis patients at the endoscopic remission stages (2.8 ± 1.7% vs. 18.5 ± 13.3%).</p><p><strong>Conclusion: </strong>We demonstrated that CD62L<sup>+</sup> T lymphocytes, particularly the CD45RA<sup>-</sup>CD62L<sup>+</sup> T cell subset that represents central memory T cells, were increased in the rectum of patients with ulcerative colitis. In addition, the CD56<sup>+</sup>/CD3<sup>+</sup> subset (natural killer T cells) was decreased in the rectum compared to that of less inflamed colonic mucosa. These results suggest that the enrichment of central memory T lymphocytes and the reduction of natural killer T cells in the gut mucosa are involved in the pathogenesis of ulcerative colitis.</p>","PeriodicalId":14046,"journal":{"name":"International Journal of Immunopathology and Pharmacology","volume":null,"pages":null},"PeriodicalIF":3.0000,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/37/a2/10.1177_20587384211051982.PMC8796091.pdf","citationCount":"4","resultStr":"{\"title\":\"Enriched CD45RA<sup>-</sup>CD62L<sup>+</sup> central memory T and decreased CD3<sup>+</sup>CD56<sup>+</sup> natural killer T lymphocyte subsets in the rectum of ulcerative colitis patients.\",\"authors\":\"Masaya Iwamuro, Takahide Takahashi, Natsuki Watanabe, Takehiro Tanaka, Toshihiro Inokuchi, Sakiko Hiraoka, Fumio Otsuka, Hiroyuki Okada\",\"doi\":\"10.1177/20587384211051982\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objectives: </strong>To investigate the distinctive features of lymphocytes promoting inflammation in ulcerative colitis.</p><p><strong>Methods: </strong>We performed flow cytometric analysis of peripheral blood mononuclear cells (PBMCs) and colorectal mucosa lymphocytes in ulcerative colitis patients (<i>n</i> = 13) and control patients (<i>n</i> = 5).</p><p><strong>Results: </strong>CD62L<sup>+</sup>/CD3<sup>+</sup>CD4<sup>+</sup> (35.7 ± 14.0% vs. 19.9 ± 6.4%) and CD62L<sup>+</sup>/CD3<sup>+</sup>CD4<sup>-</sup> cells (17.1 ± 17.4% vs. 2.4 ± 3.9%) were higher in the rectum of ulcerative colitis patients than in control patients. Subpopulation analysis revealed that CD45RA<sup>-</sup>CD62L<sup>+</sup>/CD3<sup>+</sup>CD4<sup>+</sup>, that is, central memory T cell fraction in CD4<sup>+</sup> T cells, was significantly increased in the rectum of ulcerative colitis, compared to that in control patients (23.3 ± 10.5% vs. 8.2 ± 4.0%). Comparison of rectum and colon samples in ulcerative colitis patients indicated that CD56<sup>+</sup>/CD3<sup>+</sup> was decreased in the rectum compared to that in the colon (11.3 ± 12.5% vs. 21.3 ± 16.5%). The ratio of CD56<sup>+</sup>/CD3<sup>+</sup> was also decreased in the rectum of active ulcerative colitis patients compared to that in ulcerative colitis patients at the endoscopic remission stages (2.8 ± 1.7% vs. 18.5 ± 13.3%).</p><p><strong>Conclusion: </strong>We demonstrated that CD62L<sup>+</sup> T lymphocytes, particularly the CD45RA<sup>-</sup>CD62L<sup>+</sup> T cell subset that represents central memory T cells, were increased in the rectum of patients with ulcerative colitis. In addition, the CD56<sup>+</sup>/CD3<sup>+</sup> subset (natural killer T cells) was decreased in the rectum compared to that of less inflamed colonic mucosa. These results suggest that the enrichment of central memory T lymphocytes and the reduction of natural killer T cells in the gut mucosa are involved in the pathogenesis of ulcerative colitis.</p>\",\"PeriodicalId\":14046,\"journal\":{\"name\":\"International Journal of Immunopathology and Pharmacology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":3.0000,\"publicationDate\":\"2022-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/37/a2/10.1177_20587384211051982.PMC8796091.pdf\",\"citationCount\":\"4\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International Journal of Immunopathology and Pharmacology\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1177/20587384211051982\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"IMMUNOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of Immunopathology and Pharmacology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1177/20587384211051982","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"IMMUNOLOGY","Score":null,"Total":0}
引用次数: 4
摘要
目的:探讨溃疡性结肠炎中淋巴细胞促炎的特点。方法:对13例溃疡性结肠炎患者(n = 13)和5例对照组(n = 5)的外周血单核细胞(PBMCs)和结直肠粘膜淋巴细胞进行流式细胞术分析。结果:溃疡性结肠炎患者直肠CD62L+/CD3+CD4+(35.7±14.0%比19.9±6.4%)和CD62L+/CD3+CD4-(17.1±17.4%比2.4±3.9%)高于对照组。亚群分析显示,溃疡性结肠炎患者直肠中CD45RA-CD62L+/CD3+CD4+,即CD4+ T细胞中的中枢记忆T细胞分数显著高于对照组(23.3±10.5% vs. 8.2±4.0%)。溃疡性结肠炎患者直肠和结肠样本比较显示,直肠CD56+/CD3+较结肠CD56+/CD3+降低(11.3±12.5%比21.3±16.5%)。与内镜缓解期溃疡性结肠炎患者相比,活动性溃疡性结肠炎患者直肠中CD56+/CD3+的比值也有所降低(2.8±1.7%比18.5±13.3%)。结论:我们证明了CD62L+ T淋巴细胞,特别是CD45RA-CD62L+ T细胞亚群(代表中枢记忆T细胞)在溃疡性结肠炎患者的直肠中增加。此外,与炎症较少的结肠粘膜相比,直肠中的CD56+/CD3+亚群(自然杀伤T细胞)减少。这些结果提示,肠道黏膜中央记忆T淋巴细胞的富集和自然杀伤T细胞的减少参与了溃疡性结肠炎的发病机制。
Enriched CD45RA-CD62L+ central memory T and decreased CD3+CD56+ natural killer T lymphocyte subsets in the rectum of ulcerative colitis patients.
Objectives: To investigate the distinctive features of lymphocytes promoting inflammation in ulcerative colitis.
Methods: We performed flow cytometric analysis of peripheral blood mononuclear cells (PBMCs) and colorectal mucosa lymphocytes in ulcerative colitis patients (n = 13) and control patients (n = 5).
Results: CD62L+/CD3+CD4+ (35.7 ± 14.0% vs. 19.9 ± 6.4%) and CD62L+/CD3+CD4- cells (17.1 ± 17.4% vs. 2.4 ± 3.9%) were higher in the rectum of ulcerative colitis patients than in control patients. Subpopulation analysis revealed that CD45RA-CD62L+/CD3+CD4+, that is, central memory T cell fraction in CD4+ T cells, was significantly increased in the rectum of ulcerative colitis, compared to that in control patients (23.3 ± 10.5% vs. 8.2 ± 4.0%). Comparison of rectum and colon samples in ulcerative colitis patients indicated that CD56+/CD3+ was decreased in the rectum compared to that in the colon (11.3 ± 12.5% vs. 21.3 ± 16.5%). The ratio of CD56+/CD3+ was also decreased in the rectum of active ulcerative colitis patients compared to that in ulcerative colitis patients at the endoscopic remission stages (2.8 ± 1.7% vs. 18.5 ± 13.3%).
Conclusion: We demonstrated that CD62L+ T lymphocytes, particularly the CD45RA-CD62L+ T cell subset that represents central memory T cells, were increased in the rectum of patients with ulcerative colitis. In addition, the CD56+/CD3+ subset (natural killer T cells) was decreased in the rectum compared to that of less inflamed colonic mucosa. These results suggest that the enrichment of central memory T lymphocytes and the reduction of natural killer T cells in the gut mucosa are involved in the pathogenesis of ulcerative colitis.
期刊介绍:
International Journal of Immunopathology and Pharmacology is an Open Access peer-reviewed journal publishing original papers describing research in the fields of immunology, pathology and pharmacology. The intention is that the journal should reflect both the experimental and clinical aspects of immunology as well as advances in the understanding of the pathology and pharmacology of the immune system.