增强溃疡性结肠炎患者直肠CD45RA-CD62L+中枢记忆T,降低CD3+CD56+自然杀伤T淋巴细胞亚群。

Masaya Iwamuro, Takahide Takahashi, Natsuki Watanabe, Takehiro Tanaka, Toshihiro Inokuchi, Sakiko Hiraoka, Fumio Otsuka, Hiroyuki Okada
{"title":"增强溃疡性结肠炎患者直肠CD45RA-CD62L+中枢记忆T,降低CD3+CD56+自然杀伤T淋巴细胞亚群。","authors":"Masaya Iwamuro,&nbsp;Takahide Takahashi,&nbsp;Natsuki Watanabe,&nbsp;Takehiro Tanaka,&nbsp;Toshihiro Inokuchi,&nbsp;Sakiko Hiraoka,&nbsp;Fumio Otsuka,&nbsp;Hiroyuki Okada","doi":"10.1177/20587384211051982","DOIUrl":null,"url":null,"abstract":"<p><strong>Objectives: </strong>To investigate the distinctive features of lymphocytes promoting inflammation in ulcerative colitis.</p><p><strong>Methods: </strong>We performed flow cytometric analysis of peripheral blood mononuclear cells (PBMCs) and colorectal mucosa lymphocytes in ulcerative colitis patients (<i>n</i> = 13) and control patients (<i>n</i> = 5).</p><p><strong>Results: </strong>CD62L<sup>+</sup>/CD3<sup>+</sup>CD4<sup>+</sup> (35.7 ± 14.0% vs. 19.9 ± 6.4%) and CD62L<sup>+</sup>/CD3<sup>+</sup>CD4<sup>-</sup> cells (17.1 ± 17.4% vs. 2.4 ± 3.9%) were higher in the rectum of ulcerative colitis patients than in control patients. Subpopulation analysis revealed that CD45RA<sup>-</sup>CD62L<sup>+</sup>/CD3<sup>+</sup>CD4<sup>+</sup>, that is, central memory T cell fraction in CD4<sup>+</sup> T cells, was significantly increased in the rectum of ulcerative colitis, compared to that in control patients (23.3 ± 10.5% vs. 8.2 ± 4.0%). Comparison of rectum and colon samples in ulcerative colitis patients indicated that CD56<sup>+</sup>/CD3<sup>+</sup> was decreased in the rectum compared to that in the colon (11.3 ± 12.5% vs. 21.3 ± 16.5%). The ratio of CD56<sup>+</sup>/CD3<sup>+</sup> was also decreased in the rectum of active ulcerative colitis patients compared to that in ulcerative colitis patients at the endoscopic remission stages (2.8 ± 1.7% vs. 18.5 ± 13.3%).</p><p><strong>Conclusion: </strong>We demonstrated that CD62L<sup>+</sup> T lymphocytes, particularly the CD45RA<sup>-</sup>CD62L<sup>+</sup> T cell subset that represents central memory T cells, were increased in the rectum of patients with ulcerative colitis. In addition, the CD56<sup>+</sup>/CD3<sup>+</sup> subset (natural killer T cells) was decreased in the rectum compared to that of less inflamed colonic mucosa. These results suggest that the enrichment of central memory T lymphocytes and the reduction of natural killer T cells in the gut mucosa are involved in the pathogenesis of ulcerative colitis.</p>","PeriodicalId":14046,"journal":{"name":"International Journal of Immunopathology and Pharmacology","volume":"36 ","pages":"20587384211051982"},"PeriodicalIF":3.0000,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/37/a2/10.1177_20587384211051982.PMC8796091.pdf","citationCount":"4","resultStr":"{\"title\":\"Enriched CD45RA<sup>-</sup>CD62L<sup>+</sup> central memory T and decreased CD3<sup>+</sup>CD56<sup>+</sup> natural killer T lymphocyte subsets in the rectum of ulcerative colitis patients.\",\"authors\":\"Masaya Iwamuro,&nbsp;Takahide Takahashi,&nbsp;Natsuki Watanabe,&nbsp;Takehiro Tanaka,&nbsp;Toshihiro Inokuchi,&nbsp;Sakiko Hiraoka,&nbsp;Fumio Otsuka,&nbsp;Hiroyuki Okada\",\"doi\":\"10.1177/20587384211051982\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objectives: </strong>To investigate the distinctive features of lymphocytes promoting inflammation in ulcerative colitis.</p><p><strong>Methods: </strong>We performed flow cytometric analysis of peripheral blood mononuclear cells (PBMCs) and colorectal mucosa lymphocytes in ulcerative colitis patients (<i>n</i> = 13) and control patients (<i>n</i> = 5).</p><p><strong>Results: </strong>CD62L<sup>+</sup>/CD3<sup>+</sup>CD4<sup>+</sup> (35.7 ± 14.0% vs. 19.9 ± 6.4%) and CD62L<sup>+</sup>/CD3<sup>+</sup>CD4<sup>-</sup> cells (17.1 ± 17.4% vs. 2.4 ± 3.9%) were higher in the rectum of ulcerative colitis patients than in control patients. Subpopulation analysis revealed that CD45RA<sup>-</sup>CD62L<sup>+</sup>/CD3<sup>+</sup>CD4<sup>+</sup>, that is, central memory T cell fraction in CD4<sup>+</sup> T cells, was significantly increased in the rectum of ulcerative colitis, compared to that in control patients (23.3 ± 10.5% vs. 8.2 ± 4.0%). Comparison of rectum and colon samples in ulcerative colitis patients indicated that CD56<sup>+</sup>/CD3<sup>+</sup> was decreased in the rectum compared to that in the colon (11.3 ± 12.5% vs. 21.3 ± 16.5%). The ratio of CD56<sup>+</sup>/CD3<sup>+</sup> was also decreased in the rectum of active ulcerative colitis patients compared to that in ulcerative colitis patients at the endoscopic remission stages (2.8 ± 1.7% vs. 18.5 ± 13.3%).</p><p><strong>Conclusion: </strong>We demonstrated that CD62L<sup>+</sup> T lymphocytes, particularly the CD45RA<sup>-</sup>CD62L<sup>+</sup> T cell subset that represents central memory T cells, were increased in the rectum of patients with ulcerative colitis. In addition, the CD56<sup>+</sup>/CD3<sup>+</sup> subset (natural killer T cells) was decreased in the rectum compared to that of less inflamed colonic mucosa. These results suggest that the enrichment of central memory T lymphocytes and the reduction of natural killer T cells in the gut mucosa are involved in the pathogenesis of ulcerative colitis.</p>\",\"PeriodicalId\":14046,\"journal\":{\"name\":\"International Journal of Immunopathology and Pharmacology\",\"volume\":\"36 \",\"pages\":\"20587384211051982\"},\"PeriodicalIF\":3.0000,\"publicationDate\":\"2022-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/37/a2/10.1177_20587384211051982.PMC8796091.pdf\",\"citationCount\":\"4\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International Journal of Immunopathology and Pharmacology\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1177/20587384211051982\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"IMMUNOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of Immunopathology and Pharmacology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1177/20587384211051982","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"IMMUNOLOGY","Score":null,"Total":0}
引用次数: 4

摘要

目的:探讨溃疡性结肠炎中淋巴细胞促炎的特点。方法:对13例溃疡性结肠炎患者(n = 13)和5例对照组(n = 5)的外周血单核细胞(PBMCs)和结直肠粘膜淋巴细胞进行流式细胞术分析。结果:溃疡性结肠炎患者直肠CD62L+/CD3+CD4+(35.7±14.0%比19.9±6.4%)和CD62L+/CD3+CD4-(17.1±17.4%比2.4±3.9%)高于对照组。亚群分析显示,溃疡性结肠炎患者直肠中CD45RA-CD62L+/CD3+CD4+,即CD4+ T细胞中的中枢记忆T细胞分数显著高于对照组(23.3±10.5% vs. 8.2±4.0%)。溃疡性结肠炎患者直肠和结肠样本比较显示,直肠CD56+/CD3+较结肠CD56+/CD3+降低(11.3±12.5%比21.3±16.5%)。与内镜缓解期溃疡性结肠炎患者相比,活动性溃疡性结肠炎患者直肠中CD56+/CD3+的比值也有所降低(2.8±1.7%比18.5±13.3%)。结论:我们证明了CD62L+ T淋巴细胞,特别是CD45RA-CD62L+ T细胞亚群(代表中枢记忆T细胞)在溃疡性结肠炎患者的直肠中增加。此外,与炎症较少的结肠粘膜相比,直肠中的CD56+/CD3+亚群(自然杀伤T细胞)减少。这些结果提示,肠道黏膜中央记忆T淋巴细胞的富集和自然杀伤T细胞的减少参与了溃疡性结肠炎的发病机制。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

摘要图片

摘要图片

摘要图片

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
Enriched CD45RA-CD62L+ central memory T and decreased CD3+CD56+ natural killer T lymphocyte subsets in the rectum of ulcerative colitis patients.

Objectives: To investigate the distinctive features of lymphocytes promoting inflammation in ulcerative colitis.

Methods: We performed flow cytometric analysis of peripheral blood mononuclear cells (PBMCs) and colorectal mucosa lymphocytes in ulcerative colitis patients (n = 13) and control patients (n = 5).

Results: CD62L+/CD3+CD4+ (35.7 ± 14.0% vs. 19.9 ± 6.4%) and CD62L+/CD3+CD4- cells (17.1 ± 17.4% vs. 2.4 ± 3.9%) were higher in the rectum of ulcerative colitis patients than in control patients. Subpopulation analysis revealed that CD45RA-CD62L+/CD3+CD4+, that is, central memory T cell fraction in CD4+ T cells, was significantly increased in the rectum of ulcerative colitis, compared to that in control patients (23.3 ± 10.5% vs. 8.2 ± 4.0%). Comparison of rectum and colon samples in ulcerative colitis patients indicated that CD56+/CD3+ was decreased in the rectum compared to that in the colon (11.3 ± 12.5% vs. 21.3 ± 16.5%). The ratio of CD56+/CD3+ was also decreased in the rectum of active ulcerative colitis patients compared to that in ulcerative colitis patients at the endoscopic remission stages (2.8 ± 1.7% vs. 18.5 ± 13.3%).

Conclusion: We demonstrated that CD62L+ T lymphocytes, particularly the CD45RA-CD62L+ T cell subset that represents central memory T cells, were increased in the rectum of patients with ulcerative colitis. In addition, the CD56+/CD3+ subset (natural killer T cells) was decreased in the rectum compared to that of less inflamed colonic mucosa. These results suggest that the enrichment of central memory T lymphocytes and the reduction of natural killer T cells in the gut mucosa are involved in the pathogenesis of ulcerative colitis.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
CiteScore
4.00
自引率
0.00%
发文量
88
审稿时长
15 weeks
期刊介绍: International Journal of Immunopathology and Pharmacology is an Open Access peer-reviewed journal publishing original papers describing research in the fields of immunology, pathology and pharmacology. The intention is that the journal should reflect both the experimental and clinical aspects of immunology as well as advances in the understanding of the pathology and pharmacology of the immune system.
期刊最新文献
Resveratrol promotes the differentiation of human umbilical cord mesenchymal stem cells into esophageal fibroblasts via AKT signaling pathway Clinical role of NDRG2-based methylation status on survival pattern of glioblastoma A novel immune cell signature for predicting glioblastoma after radiotherapy prognosis and guiding therapy Low-dose metformin suppresses hepatocellular carcinoma metastasis via the AMPK/JNK/IL-8 pathway Macrophage-derived exosomal miRNA-141 triggers endothelial cell pyroptosis by targeting NLRP3 to accelerate sepsis progression
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1