Katharina B. Hagen, Kelly Marie Lima, June Ang, Federico Montealegre-Golcher, Flavio H. Alonso, Esteban Soto
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引用次数: 0
摘要
transmontanus白鲟鱼是为了人类消费和保护目的而养殖的。在这项研究中,将两种市售的便携式葡萄糖和乳酸测量设备与台式分析仪进行了比较,并使用从43只健康白鲟幼鱼身上收集的肝素血浆生成了血液参考区间。生成的正常范围用于比较攻毒后10、20和30 d (dpc)感染白鲟的血浆值。在43只健康的一岁雏鸡中,将便携式设备与台式分析仪进行比较,得出了显著不同的葡萄糖和乳酸值。在便携式设备中,血糖表现出12.3 mg/dL的一致性偏差,血乳酸表现出比例偏差。在20和30 dpc时使用台式分析仪和在20 dpc时使用便携式设备检测感染鲟鱼的血糖值与未感染对照有显著差异。然而,在任何一种设备上,感染个体和对照组的血糖都在参考区间内。受感染鲟鱼的乳酸测量值无显著差异。这项研究的结果表明,便携式设备的血糖和血乳酸评估白鲟可能是有用的设置,相对值是一个可接受的近似绝对值。
Glucose and Lactate Reference Intervals for White Sturgeon and Evaluation of Two Point-of-Care Devices in Sturgeon Infected with Veronaea botryosa
White Sturgeon Acipenser transmontanus are cultured for human consumption as well as for conservation purposes. In this study, two commercially available portable devices for measuring glucose and lactate were compared to a benchtop analyzer and blood reference intervals were generated using heparin plasma collected from 43 healthy White Sturgeon yearlings. The generated normal ranges were used to compare plasma values collected from Veronaea botryosa-infected White Sturgeon at 10, 20, and 30 d postchallenge (dpc). In the 43 healthy yearlings, significantly different glucose and lactate values were obtained when comparing the portable devices to the benchtop analyzer. In the portable devices, blood glucose showed a consistent bias of 12.3 mg/dL and blood lactate showed a proportional bias. The detected blood glucose values in infected sturgeon were significantly different from those in noninfected controls when using the benchtop analyzer at 20 and 30 dpc and when using the portable device at 20 dpc. However, blood glucose in infected individuals and controls was within the reference interval on either device. No significant difference was noted for lactate measurements in infected sturgeon. The results of this study indicate that portable devices for blood glucose and blood lactate evaluation in White Sturgeon may be useful in a setting where relative values are an acceptable approximation of absolute values.
期刊介绍:
The Journal of Aquatic Animal Health serves the international community of scientists and culturists concerned with the health of aquatic organisms. It carries research papers on the causes, effects, treatments, and prevention of diseases of marine and freshwater organisms, particularly fish and shellfish. In addition, it contains papers that describe biochemical and physiological investigations into fish health that relate to assessing the impacts of both environmental and pathogenic features.