基于海藻酸盐的三维细胞培养技术评估半最大抑制浓度:甲状腺间变性癌抗癌药物研究的体外模型。

IF 1.9 Q3 ENDOCRINOLOGY & METABOLISM Thyroid Research Pub Date : 2021-12-03 DOI:10.1186/s13044-021-00118-w
Hilda Samimi, Alireza Naderi Sohi, Shiva Irani, Ehsan Arefian, Mojdeh Mahdiannasser, Parviz Fallah, Vahid Haghpanah
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引用次数: 5

摘要

背景:三维(3D)细胞培养方法可以模拟生物微环境,并显示出与体内环境更多的相似性。甲状腺间变性癌(ATC)是一种致死性内分泌恶性肿瘤。尽管采用了不同的治疗方法,但没有显示出患者存活率的改善。本研究采用三维体外ATC模型,研究BI-847325抗癌药物对二维(2D)和三维培养细胞的细胞毒作用。方法:用1%海藻酸钠溶液培养人ATC细胞株C643和SW1736。球体在培养基中孵育一周。对制备藻酸盐微球的重现性进行了评价。DAPI(4′,6-二氨基-2-苯基吲哚)染色检测细胞在海藻酸盐中的包封情况。采用CFSE(5,6-羧基荧光素n -羟基琥珀酰亚胺酯)染色评价海藻酸盐包被细胞的存活率。计算C643和SW1736细胞系在二维单层和三维体系中培养的群体倍增率。采用MTT[3-(4,5-二甲基噻唑-2)-2,5-二苯基溴化四唑]测定BI-847325对2D和3D培养细胞系24-72 h的细胞毒作用。最后,将三维培养结果与二维培养方法进行比较。结果:BI-847325在3D培养中的半最大抑制浓度(IC50)值高于2D培养。细胞毒性数据显示3D体外模型对化疗药物的耐药性更强。结论:本研究结果有助于建立体外ATC三维模型,分析不同化疗药物和制剂的疗效。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Alginate-based 3D cell culture technique to evaluate the half-maximal inhibitory concentration: an in vitro model of anticancer drug study for anaplastic thyroid carcinoma.

Background: Three-dimensional (3D) cell culture methods are identified for simulating the biological microenvironment and demonstrating more similarity to in vivo circumstances. Anaplastic thyroid carcinoma (ATC) is a lethal endocrine malignancy. Despite different treatment approaches, no improvement in the survival rate of the patients has been shown. In this study, we used the 3D in vitro ATC model to investigate the cytotoxic effect of BI-847325 anticancer drug in two-dimensional (2D)- and 3D- cultured cells.

Methods: Human ATC cell lines, C643 and SW1736, were cultured in one percentage (w/v) sodium alginate. Spheroids were incubated in medium for one week. The reproducibility of the fabrication of alginate beads was evaluated. Encapsulation of the cells in alginate was examined by DAPI (4',6-diamidino-2-phenylindole) staining. Survival of alginate-encapsulated cells was evaluated by CFSE (5,6-Carboxyfluorescein N-hydroxysuccinimidyl ester) staining. The population doubling times of C643 and SW1736 cell lines cultured in 2D monolayer as well as in 3D system were calculated. The cytotoxic effect of BI-847325 on 2D- and 3D- cultured cell lines was assessed for 24-72 h by MTT [3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide] assay. Finally, the 3D culture results were compared with the 2D culture method.

Results: The half-maximal inhibitory concentration (IC50) values of BI-847325 were higher in 3D culture compared to 2D culture. The cytotoxicity data indicated that 3D in vitro models were more resistant to chemotherapy agents.

Conclusions: The findings of this study are beneficial for developing in vitro ATC 3D models to analyze the efficacy of different chemotherapy drugs and formulations.

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来源期刊
Thyroid Research
Thyroid Research Medicine-Endocrinology, Diabetes and Metabolism
CiteScore
3.10
自引率
4.50%
发文量
21
审稿时长
8 weeks
期刊最新文献
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