{"title":"丙戊酸对无性腺和性腺切除大鼠胸腺细胞中 SLC5A8 表达的影响","authors":"Milda Juknevičienė, Ingrida Balnytė, Angelija Valančiūtė, Jūratė Stanevičiūtė, Kęstutis Sužiedėlis, Donatas Stakišaitis","doi":"10.1177/20587384211051954","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Valproic acid (VPA) pharmacological mechanisms are related to the anti-inflammatory and anti-viral effects. VPA is a histone deacetylases inhibitor and serves a role in its immunomodulatory impacts. VPA has complex effects on immune cell's mitochondrial metabolism. The SLC5A8 transporter of short fatty acids has an active role in regulating mitochondrial metabolism. The study aimed to investigate whether SLC5A8 expresses the sex-related difference and how SLC5A8 expression depends on gonadal hormones, VPA treatment, and NKCC1 expression in rat thymocytes.</p><p><strong>Methods: </strong>Control groups and VPA-treated gonad-intact and gonadectomized Wistar male and female rats were investigated (<i>n</i> = 6 in a group). The VPA 300 mg/kg/day in drinking water was given for 4 weeks. The SLC5A8 (<i>Slc5a8</i> gene) and NKCC1 (<i>Slc12a2</i> gene) RNA expressions were determined by the RT-PCR method.</p><p><strong>Results: </strong>The higher <i>Slc5a8</i> expression was found in the gonad-intact males than respective females (<i>p</i> = 0.004). VPA treatment decreased the Slc5a8 expression in gonad-intact and castrated males (<i>p</i> = 0.02 and <i>p</i> = 0.03, respectively), and increased in gonad-intact female rats compared to their control (<i>p</i> = 0.03). No significant difference in the <i>Slc5a8</i> expression between the ovariectomized female control and VPA-treated females was determined (<i>p</i> > 0.05). VPA treatment alters the correlation between <i>Sl</i>c<i>5a8</i> and Slc12a2 gene expression in thymocytes of gonad-intact rats.</p><p><strong>Conclusion: </strong>VPA effect on the <i>Slc5a8</i> expression in rat thymocytes is gender- and gonadal hormone-dependent.</p>","PeriodicalId":14046,"journal":{"name":"International Journal of Immunopathology and Pharmacology","volume":"36 ","pages":"20587384211051954"},"PeriodicalIF":3.0000,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/6e/0b/10.1177_20587384211051954.PMC8819739.pdf","citationCount":"0","resultStr":"{\"title\":\"The effect of valproic acid on SLC5A8 expression in gonad-intact and gonadectomized rat thymocytes.\",\"authors\":\"Milda Juknevičienė, Ingrida Balnytė, Angelija Valančiūtė, Jūratė Stanevičiūtė, Kęstutis Sužiedėlis, Donatas Stakišaitis\",\"doi\":\"10.1177/20587384211051954\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Valproic acid (VPA) pharmacological mechanisms are related to the anti-inflammatory and anti-viral effects. VPA is a histone deacetylases inhibitor and serves a role in its immunomodulatory impacts. VPA has complex effects on immune cell's mitochondrial metabolism. The SLC5A8 transporter of short fatty acids has an active role in regulating mitochondrial metabolism. The study aimed to investigate whether SLC5A8 expresses the sex-related difference and how SLC5A8 expression depends on gonadal hormones, VPA treatment, and NKCC1 expression in rat thymocytes.</p><p><strong>Methods: </strong>Control groups and VPA-treated gonad-intact and gonadectomized Wistar male and female rats were investigated (<i>n</i> = 6 in a group). The VPA 300 mg/kg/day in drinking water was given for 4 weeks. The SLC5A8 (<i>Slc5a8</i> gene) and NKCC1 (<i>Slc12a2</i> gene) RNA expressions were determined by the RT-PCR method.</p><p><strong>Results: </strong>The higher <i>Slc5a8</i> expression was found in the gonad-intact males than respective females (<i>p</i> = 0.004). VPA treatment decreased the Slc5a8 expression in gonad-intact and castrated males (<i>p</i> = 0.02 and <i>p</i> = 0.03, respectively), and increased in gonad-intact female rats compared to their control (<i>p</i> = 0.03). No significant difference in the <i>Slc5a8</i> expression between the ovariectomized female control and VPA-treated females was determined (<i>p</i> > 0.05). VPA treatment alters the correlation between <i>Sl</i>c<i>5a8</i> and Slc12a2 gene expression in thymocytes of gonad-intact rats.</p><p><strong>Conclusion: </strong>VPA effect on the <i>Slc5a8</i> expression in rat thymocytes is gender- and gonadal hormone-dependent.</p>\",\"PeriodicalId\":14046,\"journal\":{\"name\":\"International Journal of Immunopathology and Pharmacology\",\"volume\":\"36 \",\"pages\":\"20587384211051954\"},\"PeriodicalIF\":3.0000,\"publicationDate\":\"2022-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/6e/0b/10.1177_20587384211051954.PMC8819739.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International Journal of Immunopathology and Pharmacology\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1177/20587384211051954\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"IMMUNOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of Immunopathology and Pharmacology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1177/20587384211051954","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"IMMUNOLOGY","Score":null,"Total":0}
The effect of valproic acid on SLC5A8 expression in gonad-intact and gonadectomized rat thymocytes.
Background: Valproic acid (VPA) pharmacological mechanisms are related to the anti-inflammatory and anti-viral effects. VPA is a histone deacetylases inhibitor and serves a role in its immunomodulatory impacts. VPA has complex effects on immune cell's mitochondrial metabolism. The SLC5A8 transporter of short fatty acids has an active role in regulating mitochondrial metabolism. The study aimed to investigate whether SLC5A8 expresses the sex-related difference and how SLC5A8 expression depends on gonadal hormones, VPA treatment, and NKCC1 expression in rat thymocytes.
Methods: Control groups and VPA-treated gonad-intact and gonadectomized Wistar male and female rats were investigated (n = 6 in a group). The VPA 300 mg/kg/day in drinking water was given for 4 weeks. The SLC5A8 (Slc5a8 gene) and NKCC1 (Slc12a2 gene) RNA expressions were determined by the RT-PCR method.
Results: The higher Slc5a8 expression was found in the gonad-intact males than respective females (p = 0.004). VPA treatment decreased the Slc5a8 expression in gonad-intact and castrated males (p = 0.02 and p = 0.03, respectively), and increased in gonad-intact female rats compared to their control (p = 0.03). No significant difference in the Slc5a8 expression between the ovariectomized female control and VPA-treated females was determined (p > 0.05). VPA treatment alters the correlation between Slc5a8 and Slc12a2 gene expression in thymocytes of gonad-intact rats.
Conclusion: VPA effect on the Slc5a8 expression in rat thymocytes is gender- and gonadal hormone-dependent.
期刊介绍：
International Journal of Immunopathology and Pharmacology is an Open Access peer-reviewed journal publishing original papers describing research in the fields of immunology, pathology and pharmacology. The intention is that the journal should reflect both the experimental and clinical aspects of immunology as well as advances in the understanding of the pathology and pharmacology of the immune system.
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