{"title":"Wnt/PCP通路在体外调控间充质干细胞的迁移和神经分化。","authors":"Panpan Yao, Qin Yu, Lujie Zhu, Jingxian Li, Xueyuan Zhou, Lili Wu, Yongyi Cai, Hongmei Shen, Liping Zhou","doi":"10.5603/FHC.a2022.0006","DOIUrl":null,"url":null,"abstract":"<p><strong>Introduction: </strong>Mesenchymal stem cells (MSCs) are an excellent donor graft source due to their potential for self-renewal and multidirectional differentiation. However, the potential mechanisms involved in MSC homing and neural differentiation are still unclear. The purpose of this study was to explore the effects of a chemokine, SDF-1a, and Wnt3a ligand on rat MSCs' migration and b-mercaptoethanol (BME)-induced neural differentiation of MSCs.</p><p><strong>Materials and methods: </strong>MSCs were isolated from rat bone marrow and cultured in vitro to passage 3. Scratch tests and transwell assays were used to estimate the effects of SDF-1a (25 ng/mL) and Wnt3a (10 ng/mL) on the migration of MSCs. The expression of Wnt/PCP pathway proteins RhoA, c-Jun, ATF2, and Wnt3a were assessed by Western blot. The 5 mM BME-induced neural differentiation of MSCs was determined by immunofluorescence to detect neuron- and astrocyte-specific markers such as nestin, GFAP, and Olig2.</p><p><strong>Results: </strong>Wnt3a promoted the migration ability of MSCs and regulated the expression of RhoA, c-Jun, and ATF2 proteins. MSCs could differentiate into neural stem cells and astrocytes. Wnt3a enhanced BME induced neurogenesis in MSCs by increasing the protein expression of RhoA, c-Jun, and Wnt3a.</p><p><strong>Conclusions: </strong>The present study demonstrated that the Wnt/PCP pathway promotes migration and neural differentiation of rat MSC.</p>","PeriodicalId":1,"journal":{"name":"Accounts of Chemical Research","volume":null,"pages":null},"PeriodicalIF":16.4000,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"Wnt/PCP pathway regulates the migration and neural differentiation of mesenchymal stem cells in vitro.\",\"authors\":\"Panpan Yao, Qin Yu, Lujie Zhu, Jingxian Li, Xueyuan Zhou, Lili Wu, Yongyi Cai, Hongmei Shen, Liping Zhou\",\"doi\":\"10.5603/FHC.a2022.0006\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Introduction: </strong>Mesenchymal stem cells (MSCs) are an excellent donor graft source due to their potential for self-renewal and multidirectional differentiation. However, the potential mechanisms involved in MSC homing and neural differentiation are still unclear. The purpose of this study was to explore the effects of a chemokine, SDF-1a, and Wnt3a ligand on rat MSCs' migration and b-mercaptoethanol (BME)-induced neural differentiation of MSCs.</p><p><strong>Materials and methods: </strong>MSCs were isolated from rat bone marrow and cultured in vitro to passage 3. Scratch tests and transwell assays were used to estimate the effects of SDF-1a (25 ng/mL) and Wnt3a (10 ng/mL) on the migration of MSCs. The expression of Wnt/PCP pathway proteins RhoA, c-Jun, ATF2, and Wnt3a were assessed by Western blot. The 5 mM BME-induced neural differentiation of MSCs was determined by immunofluorescence to detect neuron- and astrocyte-specific markers such as nestin, GFAP, and Olig2.</p><p><strong>Results: </strong>Wnt3a promoted the migration ability of MSCs and regulated the expression of RhoA, c-Jun, and ATF2 proteins. MSCs could differentiate into neural stem cells and astrocytes. Wnt3a enhanced BME induced neurogenesis in MSCs by increasing the protein expression of RhoA, c-Jun, and Wnt3a.</p><p><strong>Conclusions: </strong>The present study demonstrated that the Wnt/PCP pathway promotes migration and neural differentiation of rat MSC.</p>\",\"PeriodicalId\":1,\"journal\":{\"name\":\"Accounts of Chemical Research\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":16.4000,\"publicationDate\":\"2022-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Accounts of Chemical Research\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.5603/FHC.a2022.0006\",\"RegionNum\":1,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2022/2/14 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q1\",\"JCRName\":\"CHEMISTRY, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Accounts of Chemical Research","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.5603/FHC.a2022.0006","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2022/2/14 0:00:00","PubModel":"Epub","JCR":"Q1","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}
引用次数: 1
摘要
间充质干细胞(MSCs)具有自我更新和多向分化的潜力，是一种很好的移植供体来源。然而，MSC归巢和神经分化的潜在机制尚不清楚。本研究旨在探讨趋化因子SDF-1a和Wnt3a配体对大鼠间充质干细胞迁移和b-巯基乙醇(BME)诱导的间充质干细胞神经分化的影响。材料和方法:从大鼠骨髓中分离MSCs，体外培养至传代3。采用划痕实验和transwell实验来评估SDF-1a (25 ng/mL)和Wnt3a (10 ng/mL)对MSCs迁移的影响。Western blot检测Wnt/PCP通路蛋白RhoA、c-Jun、ATF2、Wnt3a的表达。通过免疫荧光检测神经元和星形胶质细胞特异性标记物，如巢蛋白、GFAP和Olig2，检测5 mM bme诱导的MSCs神经分化。结果:Wnt3a能促进MSCs的迁移能力，调节RhoA、c-Jun、ATF2蛋白的表达。MSCs可分化为神经干细胞和星形胶质细胞。Wnt3a通过增加RhoA、c-Jun和Wnt3a的蛋白表达，增强BME诱导的MSCs神经发生。结论:Wnt/PCP通路促进大鼠间充质干细胞的迁移和神经分化。
Wnt/PCP pathway regulates the migration and neural differentiation of mesenchymal stem cells in vitro.
Introduction: Mesenchymal stem cells (MSCs) are an excellent donor graft source due to their potential for self-renewal and multidirectional differentiation. However, the potential mechanisms involved in MSC homing and neural differentiation are still unclear. The purpose of this study was to explore the effects of a chemokine, SDF-1a, and Wnt3a ligand on rat MSCs' migration and b-mercaptoethanol (BME)-induced neural differentiation of MSCs.
Materials and methods: MSCs were isolated from rat bone marrow and cultured in vitro to passage 3. Scratch tests and transwell assays were used to estimate the effects of SDF-1a (25 ng/mL) and Wnt3a (10 ng/mL) on the migration of MSCs. The expression of Wnt/PCP pathway proteins RhoA, c-Jun, ATF2, and Wnt3a were assessed by Western blot. The 5 mM BME-induced neural differentiation of MSCs was determined by immunofluorescence to detect neuron- and astrocyte-specific markers such as nestin, GFAP, and Olig2.
Results: Wnt3a promoted the migration ability of MSCs and regulated the expression of RhoA, c-Jun, and ATF2 proteins. MSCs could differentiate into neural stem cells and astrocytes. Wnt3a enhanced BME induced neurogenesis in MSCs by increasing the protein expression of RhoA, c-Jun, and Wnt3a.
Conclusions: The present study demonstrated that the Wnt/PCP pathway promotes migration and neural differentiation of rat MSC.
期刊介绍：
Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance.
Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.
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