眼营养不良大疱性表皮松解症小鼠角膜变化及改善生存的策略。

IF 1.3 4区 农林科学 Q2 VETERINARY SCIENCES Comparative medicine Pub Date : 2022-02-01 Epub Date: 2022-02-07 DOI:10.30802/AALAS-CM-21-000063
Vicki M Chen, Karrie Southwell, Erin Huynh, Stefanie Gavett, Lauren Richey, Michael Esmail
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摘要

由于该菌株公布的断奶存活率为24%,因此对CVII胶原蛋白半成型小鼠的眼科研究具有独特的挑战性。由于慢性眼纤维化的发展需要时间,因此优化生存率至关重要。在本研究中,通过添加灭菌饲料和药物输送凝胶、酸化水、辐照食物颗粒、纤维素纤维垫料、最小化处理、在出生后2-3周内将兄弟姐妹移除以及优选的住房位置,提高了标准的饲养实践。在43个月的时间里,记录和分析了每个繁殖周期、性别、体重和早期安乐死原因的存活率。总体而言,49%的小鼠存活到断奶,76%的断奶小鼠存活到20周龄。到20周时,65%的小鼠出现角膜混浊,但只有10%的眼睛出现持续混浊,这表明存在纤维化。角膜混浊的发生率与大疱性表皮松解症患者相同。66%的小鼠在第11周时体重减轻。男性需要早期安乐死的几率是女性的4倍。88%的男性因阴茎脱垂导致尿路梗阻需要安乐死。在我们的强化护理方案下,在我们的群体中,拟态小鼠的存活率是公布的两倍。有了这一修订后的饲养标准,计划终止终点为雄性14周和雌性17周的实验更有可能完成。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Corneal Changes and Strategies to Improve Survival of Hypomorphic Collagen VII-Deficient Mice for the Study of Ocular Dystrophic Epidermolysis Bullosa.

Ophthalmic study of collagen CVII hypomorphic mice is uniquely challenging due to the strain's published survival rate to weaning of 24%. Because chronic ocular fibrosis requires time to develop, optimizing the survival rate is of critical importance. In this study, standard husbandry practices were enhanced by the addition of sterilized diet and drug delivery gels, acidified water, irradiated food pellets, cellulose fiber bedding, minimal handling, removal of siblings within 2-3 wk from birth, and a preferred housing location. Survival rates per breeding cycle, sex, weight, and cause of early euthanasia were recorded and analyzed over 43 mo. Overall, 49% of mice survived to weaning and 76% of weaned mice survived to 20 wk of age. Corneal opacities were seen in 65% of mice by 20 wk, but only 10% of eyes showed the sustained opacification that was indicative of fibrosis. Corneal opacities occurred at the same rate as in humans with epidermolysis bullosa. 66% of the mice showed weight loss at 11 wk. Males required early euthanasia 4 times more often than did females. Euthanasia was required for urinary obstruction due to penile prolapse in 88% of males. With our enhanced care protocol, hypomorphic mice in our colony survived at twice the published rate. With this revised husbandry standard, experiments planned with termination endpoints of 14 wk for males and 17 wk for females are more likely to reach completion.

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来源期刊
Comparative medicine
Comparative medicine 医学-动物学
CiteScore
1.90
自引率
0.00%
发文量
71
审稿时长
6-12 weeks
期刊介绍: Comparative Medicine (CM), an international journal of comparative and experimental medicine, is the leading English-language publication in the field and is ranked by the Science Citation Index in the upper third of all scientific journals. The mission of CM is to disseminate high-quality, peer-reviewed information that expands biomedical knowledge and promotes human and animal health through the study of laboratory animal disease, animal models of disease, and basic biologic mechanisms related to disease in people and animals.
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