先天性膈疝胎兔模型肝脏病理改变。

IF 1.3 4区 医学 Q3 PEDIATRICS Congenital Anomalies Pub Date : 2022-05-01 Epub Date: 2022-02-25 DOI:10.1111/cga.12462
Gloria Pelizzo, José L Peiro, Vincenzo Villanacci, Laurenço Sbragia, Marc Oria, Annalisa De Silvestri, Emanuela Mazzon, Valeria Calcaterra
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引用次数: 3

摘要

到目前为止,胎儿肝脏的影响并不是一个很好的理解先天性膈疝(CDH)的现象。我们在CDH实验模型中评估手术后胎儿肝脏生长的形态学变化。在妊娠期E25时手术产生膈缺损,在妊娠期E27时手术产生气管闭塞(TO)。分为5个实验组:对照组、左CDH组、右CDH组、CDH + TO组和单独使用TO组。测量身体和器官的生长情况。为了对CDH效应进行组织学评价,收集肝脏切片。左- cdh组肝脏白细胞浸润较对照组增加(p = 0.02)。与右cdh组相比,左cdh组毛细血管窦充血和肝细胞空泡增多(p = 0.05)。与CDH + TO组相比,左CDH组毛细血管窦充血和间质水肿更为明显(p = 0.05)。与对照组相比,CDH + TO组鼻窦充血、肝细胞空泡和间质水肿的增加也更大(p≤0.02)。右cdh组胸内肝重量高于左cdh组(p
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Liver pathological alterations in fetal rabbit model of congenital diaphragmatic hernia.

To date, fetal liver implication is not a well-understood phenomenon in congenital diaphragmatic hernia (CDH). We evaluated the fetal morphologic changes on liver growth after surgical procedure in CDH experimental model. A diaphragmatic defect at gestational day E25 and tracheal occlusion (TO) at E27 were surgically created in rabbit fetuses. Five experimental groups were assessed: control group, left CDH, right CDH, CDH + TO, and TO alone. Body and organ growth were measured. For histological evaluation of the CDH effect, liver sections were collected. Left-CDH group had livers with increased leukocyte infiltration in comparison with controls (p = 0.02). Increased capillary sinusoid congestion and hepatocyte vacuolation were greater in left-CDH compared with the right-CDH group (p = 0.05). Capillary sinusoid congestion and interstitial edema were more evident in the left-CDH compared with CDH + TO group (p = 0.05). Increases in sinusoid congestion, hepatocyte vacuolation, and interstitial edema were also greater in the CDH + TO compared with controls (p ≤ 0.02). Intrathoracic liver weight was higher in right-CDH compared with left-CDH group (p < 0.001). Total lung weights (TLW) were significantly lower in both left-CDH compared with controls (p < 0.001), CDH + TO (p = 0.01), and TO (p < 0.01) and in right-CDH compared with CDH + TO (p < 0.01) and TO (p < 0.01). Decreased kidney and heart weights were also recorded. Hemodynamics and structural fetal liver changes in laterality were noted in CDH model. Regulation of intrathoracic liver weights seems to be disturbed by the absence of diaphragmic contact. Pulmonary injury is supported by the effect of a first hit, while the growth of internal organs suggests a multisystemic remodeling related to the fetal adaptation.

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来源期刊
Congenital Anomalies
Congenital Anomalies PEDIATRICS-
自引率
0.00%
发文量
49
审稿时长
>12 weeks
期刊介绍: Congenital Anomalies is the official English language journal of the Japanese Teratology Society, and publishes original articles in laboratory as well as clinical research in all areas of abnormal development and related fields, from all over the world. Although contributions by members of the teratology societies affiliated with The International Federation of Teratology Societies are given priority, contributions from non-members are welcomed.
期刊最新文献
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