{"title":"Aestuariivivens marinum sp.nov.、Aestuarievivens sediminis sp.nov..和Aestuariovivens sediminicola sp.novs.三个从潮坪沉积物上层分离的新种的描述。","authors":"Zhaobin Huang , Xiaowei Zhou , Qiliang Lai","doi":"10.1016/j.syapm.2022.126355","DOIUrl":null,"url":null,"abstract":"<div><p><span>Nine bacterial strains designated MT3-5-12</span><sup>T</sup>, MT3-5-27, MTV1-9, S-DT1-15<sup>T</sup>, S-DT1-34, MTV5-3<sup>T</sup><span><span>, MTV4-17, MTV5-12 and MTV5-13 were isolated from the upper layer (1–5 cm in depth) of tidal flat sediment in Quanzhou Bay, China. The 16S </span>rRNA gene of these strains shared maximum sequence similarities with </span><span><em>Aestuariivivens </em><em>insulae</em></span> KCTC 42350<sup>T</sup><span> of 94.9–97.1%. Phylogenetic analyses based on 16S rRNA gene sequences and 120 conserved concatenated proteins placed these strains in three novel phylogenetic clades affiliated to the genus </span><em>Aestuariivivens</em> of the family <span><em>Flavobacteriaceae</em></span>. Strains MT3-5-12<sup>T</sup>, MT3-5-27 and MTV1-9 were phylogenetically close to <em>A. insulae</em> KCTC 42350<sup>T</sup>. The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strains MT3-5-12<sup>T</sup>and MTV1-9 and <em>A. insulae</em> KCTC 42350<sup>T</sup> were estimated to be 78.5-78.7% and 22.5%, respectively. Strains S-DT1-15<sup>T</sup> and S-DT1-34 formed a distinctly separated clade from <em>A. insulae</em> KCTC 42350<sup>T</sup>. The ANI and dDDH values between strains S-DT1-15<sup>T</sup> and S-DT1-34 and <em>A. insulae</em> KCTC 42350<sup>T</sup> were 76.3–76.4% and 20.4–20.5%, respectively. The other four strains MTV5-3<sup>T</sup>, MTV4-17, MTV5-12 and MTV5-13, formed a third novel clade, distinctly separated from <em>A. insulae</em> KCTC 42350<sup>T</sup>. The ANI and dDDH values between strains MTV5-3<sup>T</sup> and MTV4-17 and <em>A. insulae</em> KCTC 42350<sup>T</sup> were 74.7% and 19.1–19.2%, respectively. The phylogenetic analyses and whole genomic comparisons, combined with phenotypic and chemotaxonomic features, strongly supported the nine strains could be classified as three novel species within the genus <em>Aestuariivivens</em>, for which the names <em>Aestuariivivens marinum</em> sp. nov. MT3-5-12<sup>T</sup>, <em>Aestuariivivens sediminis</em> sp. nov. S-DT1-15<sup>T</sup>, and <em>Aestuariivivens sediminicola</em> sp. nov. MTV5-3<sup>T</sup> are proposed.</p></div>","PeriodicalId":22124,"journal":{"name":"Systematic and applied microbiology","volume":null,"pages":null},"PeriodicalIF":3.3000,"publicationDate":"2022-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"Description of Aestuariivivens marinum sp. nov., Aestuariivivens sediminis sp. nov. and Aestuariivivens sediminicola sp. nov., three new species isolated from the upper layer of tidal flat sediment\",\"authors\":\"Zhaobin Huang , Xiaowei Zhou , Qiliang Lai\",\"doi\":\"10.1016/j.syapm.2022.126355\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p><span>Nine bacterial strains designated MT3-5-12</span><sup>T</sup>, MT3-5-27, MTV1-9, S-DT1-15<sup>T</sup>, S-DT1-34, MTV5-3<sup>T</sup><span><span>, MTV4-17, MTV5-12 and MTV5-13 were isolated from the upper layer (1–5 cm in depth) of tidal flat sediment in Quanzhou Bay, China. The 16S </span>rRNA gene of these strains shared maximum sequence similarities with </span><span><em>Aestuariivivens </em><em>insulae</em></span> KCTC 42350<sup>T</sup><span> of 94.9–97.1%. Phylogenetic analyses based on 16S rRNA gene sequences and 120 conserved concatenated proteins placed these strains in three novel phylogenetic clades affiliated to the genus </span><em>Aestuariivivens</em> of the family <span><em>Flavobacteriaceae</em></span>. Strains MT3-5-12<sup>T</sup>, MT3-5-27 and MTV1-9 were phylogenetically close to <em>A. insulae</em> KCTC 42350<sup>T</sup>. The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strains MT3-5-12<sup>T</sup>and MTV1-9 and <em>A. insulae</em> KCTC 42350<sup>T</sup> were estimated to be 78.5-78.7% and 22.5%, respectively. Strains S-DT1-15<sup>T</sup> and S-DT1-34 formed a distinctly separated clade from <em>A. insulae</em> KCTC 42350<sup>T</sup>. The ANI and dDDH values between strains S-DT1-15<sup>T</sup> and S-DT1-34 and <em>A. insulae</em> KCTC 42350<sup>T</sup> were 76.3–76.4% and 20.4–20.5%, respectively. The other four strains MTV5-3<sup>T</sup>, MTV4-17, MTV5-12 and MTV5-13, formed a third novel clade, distinctly separated from <em>A. insulae</em> KCTC 42350<sup>T</sup>. The ANI and dDDH values between strains MTV5-3<sup>T</sup> and MTV4-17 and <em>A. insulae</em> KCTC 42350<sup>T</sup> were 74.7% and 19.1–19.2%, respectively. The phylogenetic analyses and whole genomic comparisons, combined with phenotypic and chemotaxonomic features, strongly supported the nine strains could be classified as three novel species within the genus <em>Aestuariivivens</em>, for which the names <em>Aestuariivivens marinum</em> sp. nov. MT3-5-12<sup>T</sup>, <em>Aestuariivivens sediminis</em> sp. nov. S-DT1-15<sup>T</sup>, and <em>Aestuariivivens sediminicola</em> sp. nov. MTV5-3<sup>T</sup> are proposed.</p></div>\",\"PeriodicalId\":22124,\"journal\":{\"name\":\"Systematic and applied microbiology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":3.3000,\"publicationDate\":\"2022-11-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Systematic and applied microbiology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0723202022000625\",\"RegionNum\":2,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Systematic and applied microbiology","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0723202022000625","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
Description of Aestuariivivens marinum sp. nov., Aestuariivivens sediminis sp. nov. and Aestuariivivens sediminicola sp. nov., three new species isolated from the upper layer of tidal flat sediment
Nine bacterial strains designated MT3-5-12T, MT3-5-27, MTV1-9, S-DT1-15T, S-DT1-34, MTV5-3T, MTV4-17, MTV5-12 and MTV5-13 were isolated from the upper layer (1–5 cm in depth) of tidal flat sediment in Quanzhou Bay, China. The 16S rRNA gene of these strains shared maximum sequence similarities with Aestuariivivens insulae KCTC 42350T of 94.9–97.1%. Phylogenetic analyses based on 16S rRNA gene sequences and 120 conserved concatenated proteins placed these strains in three novel phylogenetic clades affiliated to the genus Aestuariivivens of the family Flavobacteriaceae. Strains MT3-5-12T, MT3-5-27 and MTV1-9 were phylogenetically close to A. insulae KCTC 42350T. The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strains MT3-5-12Tand MTV1-9 and A. insulae KCTC 42350T were estimated to be 78.5-78.7% and 22.5%, respectively. Strains S-DT1-15T and S-DT1-34 formed a distinctly separated clade from A. insulae KCTC 42350T. The ANI and dDDH values between strains S-DT1-15T and S-DT1-34 and A. insulae KCTC 42350T were 76.3–76.4% and 20.4–20.5%, respectively. The other four strains MTV5-3T, MTV4-17, MTV5-12 and MTV5-13, formed a third novel clade, distinctly separated from A. insulae KCTC 42350T. The ANI and dDDH values between strains MTV5-3T and MTV4-17 and A. insulae KCTC 42350T were 74.7% and 19.1–19.2%, respectively. The phylogenetic analyses and whole genomic comparisons, combined with phenotypic and chemotaxonomic features, strongly supported the nine strains could be classified as three novel species within the genus Aestuariivivens, for which the names Aestuariivivens marinum sp. nov. MT3-5-12T, Aestuariivivens sediminis sp. nov. S-DT1-15T, and Aestuariivivens sediminicola sp. nov. MTV5-3T are proposed.
期刊介绍:
Systematic and Applied Microbiology deals with various aspects of microbial diversity and systematics of prokaryotes. It focuses on Bacteria and Archaea; eukaryotic microorganisms will only be considered in rare cases. The journal perceives a broad understanding of microbial diversity and encourages the submission of manuscripts from the following branches of microbiology: