Wenxuan Wang, Tanghui Xie, Fan Jiang, Yaoxin Jiang, Zhe Jin, Zhe Yang, Mei Kong, Lei Cheng, Liangliang Fu, Xiaoyong Du
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引用次数: 0
摘要
弯血蜱(Haemonchus contortus)对世界热带和亚热带地区的畜牧业经济造成了巨大的影响。先天免疫是宿主防御的第一道防线。宿主通过多种模式识别受体(PRRs)识别病原体相关分子模式(PAMPs),并激活下游信号通路来抵抗病原体的入侵。因此,阐明宿主与病原体之间的免疫相互作用是了解宿主如何抵抗病原体的关键。利用基于重组的“Library vs. Library”酵母双杂交系统(RLL-Y2H)鉴定山羊先天免疫信号通路蛋白与弯弯鼠分泌蛋白(ESPs)之间的1516个蛋白-蛋白相互作用(PPIs),并构建了PPIs网络。其中NLR和IL-17信号通路蛋白相互作用最多。NOD1与MUC5AC蛋白之间存在较多的相互作用蛋白。结合本实验室前期工作中鉴定的易感山羊和耐药山羊的差异表达基因(DEGs),选择交叉基因构建PPIs网络,TRAF2作为山羊先天免疫信号通路的关键蛋白出现。本研究初步研究了山羊与弯形血鼠ESPs之间的PPIs,为更好地了解山羊与弯形血鼠之间的免疫相互作用提供了有价值的信息。
Protein interactions network of goat innate immune signalling pathway proteins and Haemonchus contortus excretory-secretory proteins.
Haemonchus contortus (H. contortus) has caused a huge impact on the animal husbandry economy in the world's tropical and subtropical regions. Innate immunity is the first-line of host defence. The host recognizes pathogen-associated molecular patterns (PAMPs) through a variety of pattern recognition receptors (PRRs) and activates downstream signalling pathways to resist pathogens invasion. Therefore, elucidating the immune interaction between host and pathogen is key to understanding how the host resists the pathogen. We identified 1516 protein-protein interactions (PPIs) between goat innate immune signal pathway proteins and H. contortus excretory-secretory proteins (ESPs) by Recombination-based "Library vs. Library" yeast two-hybrid system (RLL-Y2H) and constructed the PPIs network. Among them, the NLR and IL-17 signalling pathways have the most protein interactions. And there were more interaction proteins between NOD1 and MUC5AC proteins in the pathways. Combined with the differentially expressed genes (DEGs) of susceptible and resistant goats identified in the preliminary work of our laboratory, we selected the intersection genes to construct the PPIs network, and TRAF2 appeared as a key protein of goat innate immune signalling pathway. We initially studied the PPIs between goat and H. contortus ESPs, which provides valuable information for better understanding the immune interaction between the goats and the H. contortus.
期刊介绍:
Parasite Immunology is an international journal devoted to research on all aspects of parasite immunology in human and animal hosts. Emphasis has been placed on how hosts control parasites, and the immunopathological reactions which take place in the course of parasitic infections. The Journal welcomes original work on all parasites, particularly human parasitology, helminths, protozoa and ectoparasites.