嗜有机甲基杆菌在甲醇中的生长,同时生产单细胞蛋白和感兴趣的代谢物。

IF 2.3 4区 农林科学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Food Technology and Biotechnology Pub Date : 2022-09-01 DOI:10.17113/ftb.60.03.22.7372
Ana Cristina Pantoja Simões, Rodrigo Pimentel Fernandes, Maysa Silva Barreto, Gabriela Bouça Marques da Costa, Mateus Gomes de Godoy, Denise Maria Guimarães Freire, Nei Pereira
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引用次数: 0

摘要

研究背景:本研究旨在监测嗜有机甲基细菌(Methylobacterium organophilum)在以甲醇为碳源的培养基中的生长情况,验证单细胞蛋白质和其他生物分子(如类胡萝卜素、外多糖和聚羟基烷酸酯)的产生,使其成为更具吸引力的动物饲料。实验方法:在摇瓶中使用不同的碳/氮(C:N)比研究细菌生长,以确定达到最高细胞体积生产力和底物消耗率的最佳比例。该优化参数进一步应用于间歇进料操作生物反应器系统,以确定细胞生长的动力学剖面。采用高效液相色谱法测定甲醇消耗,液相色谱/质谱法测定提取的色素。测定了所制外多糖的化学组成和流变性能。结果与结论:在培养基中初始甲醇浓度为7 g/L时,验证了最佳实验参数。采用相同的初始底物浓度进行分批投料操作,培养60 h后生物量达到5 g/L。监测与细胞生长相关的类胡萝卜素的积累,在过程结束时达到1.6 mg/L的浓度。然后对这些色素进行分析并鉴定为一组叶黄素(氧化类胡萝卜素)。此外,在补料间歇操作过程中,还鉴定了另外两种产品类型:外多糖,培养结束时浓度达到8.9 g/L;透射电子显微镜(TEM)检测到细胞内颗粒结构,表明聚积了聚羟基烷酸酯(PHA),最有可能是聚羟基丁酸酯。新颖性和科学贡献:嗜有机甲基细菌展示了一种独特的生产具有商业价值的化合物的能力。这种细菌独特的代谢多样性为其在生物炼制中使用提供了空间。
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Growth of Methylobacterium organophilum in Methanol for the Simultaneous Production of Single-Cell Protein and Metabolites of Interest.

Research background: This study aims to monitor the growth of the methylotrophic bacteria Methylobacterium organophilum in a culture medium with methanol as a carbon source and to verify the production of unicellular proteins and other biomolecules, such as carotenoids, exopolysaccharides and polyhydroxyalkanoates, making them more attractive as animal feed.

Experimental approach: Bacterial growth was studied in shake flasks using different carbon/nitrogen (C:N) ratios to determine their best ratio for achieving the highest volumetric productivity of cells and substrate consumption rate. This optimal parameter was further used in a fed-batch operating bioreactor system to define the kinetic profile of cell growth. Methanol consumption was measured by HPLC analysis and the extracted pigments were analyzed by liquid chromatography/mass spectrometry. Chemical composition and rheological properties of the produced exopolysaccharides were also determined.

Results and conclusions: The best experimental parameters were verified using an initial methanol concentration of 7 g/L in the culture medium. The same initial substrate concentration was used in the fed-batch operation and after 60 h of cultivation 5 g/L of biomass were obtained. The accumulation of carotenoids associated with cell growth was monitored, reaching a concentration of 1.6 mg/L at the end of the process. These pigments were then analyzed and characterized as a set of xanthophylls (oxidized carotenoids). In addition, two other product types were identified during the fed-batch operation: exopolysaccharides, which reached a concentration of 8.9 g/L at the end of the cultivation, and an intracellular granular structure that was detected by transmission electron microscopy (TEM), suggesting the accumulation of polyhydroxyalkanoate (PHA), most likely polyhydroxybutyrate.

Novelty and scientific contribution: Methylobacterium organophilum demonstrated a unique ability to produce compounds of commercial interest. The distinct metabolic diversity of this bacterium makes room for its use in biorefineries.

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来源期刊
Food Technology and Biotechnology
Food Technology and Biotechnology 工程技术-生物工程与应用微生物
CiteScore
3.70
自引率
0.00%
发文量
33
审稿时长
12 months
期刊介绍: Food Technology and Biotechnology (FTB) is a diamond open access, peer-reviewed international quarterly scientific journal that publishes papers covering a wide range of topics, including molecular biology, genetic engineering, biochemistry, microbiology, biochemical engineering and biotechnological processing, food science, analysis of food ingredients and final products, food processing and technology, oenology and waste treatment. The Journal is published by the University of Zagreb, Faculty of Food Technology and Biotechnology, Croatia. It is an official journal of Croatian Society of Biotechnology and Slovenian Microbiological Society, financed by the Croatian Ministry of Science and Education, and supported by the Croatian Academy of Sciences and Arts.
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