LncRNA-loc391533通过血管内皮生长因子参与子痫前期的进展。

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY Accounts of Chemical Research Pub Date : 2024-01-01 Epub Date: 2022-08-09 DOI:10.5603/GP.a2022.0056

Jun Xiong, Xuan Jin, Kangxiang Xu, Bingqi Wu, Yuqi Xu, Dong Ruan, Xiaoju He

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引用次数: 0

摘要

目的：子痫前期（PE）是全球孕产妇死亡的主要原因，也是最主要的妊娠并发症之一。本研究评估了血管内皮生长因子（VEGF）和lncRNA-loc391533对子痫前期的影响：材料和方法：使用免疫组织化学和酶联免疫吸附试验（ELISA）测定血管内皮生长因子在 PE 孕妇中的表达。采用定量聚合酶链反应（qPCR）和免疫印迹法检测lncRNA-loc391533敲除和过表达对血管内皮生长因子表达的影响。进行了功能缺失/功能增益实验，以评估lncRNA-loc391533对滋养细胞HTR-8/SVneo细胞的增殖、细胞周期和迁移的作用：结果：我们发现VEGF及其受体VEGFR1/2在PE中低表达。敲除lncRNA-loc391533可提高VEGF的表达，而过表达lncRNA-loc391533则会降低VEGF的表达。此外，lncRNA-loc391533是HTR-8/SVneo细胞增殖和迁移所必需的：总之，我们的研究结果表明，lncRNA-loc391533通过血管内皮生长因子在PE的进展过程中起着关键作用，它可能是治疗PE的新靶点。

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LncRNA-loc391533 is involved in the progression of preeclampsia through VEGF.

Objectives: Preeclampsia (PE) is a leading cause of maternal death worldwide, which is one of the most major pregnancy complications. The effects of vascular endothelial growth factor (VEGF) and lncRNA-loc391533 on PE were evaluated in the present study.

Material and methods: Expression of VEGF in pregnant women with PE was determined using immunohistochemical and enzyme linked immunosorbent assay (ELISA). The effects of lncRNA-loc391533 knockdown and overexpression on VEGF expression was detected using quantitative polymerase chain reaction (qPCR) and western blotting. Loss/gain-of-function assays were performed to evaluate the role of lncRNA-loc391533 on proliferation, cell cycle and migration of trophoblasts HTR-8/SVneo cells.

Results: We found that VEGF and its receptor VEGFR1/2 were low expressed in PE. Knockdown of lncRNA-loc391533 enhanced VEGF expression, while overexpression of lncRNA-loc391533 downregulated VEGF. Moreover, lncRNA-loc391533 was required for proliferation and migration of HTR-8/SVneo cells.

Conclusions: In conclusion, our findings emphasized that lncRNA-loc391533 exhibited a critical role in progression of PE through VEGF, which might as a novel therapeutic target for PE treatment.

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.