利用交流放大器记录ERG c波，评价大鼠视网膜色素上皮细胞的功能。

IF 2.6 4区医学 Q2 OPHTHALMOLOGY Documenta Ophthalmologica Pub Date : 2022-10-01 Epub Date: 2022-07-27 DOI:10.1007/s10633-022-09890-y

Naohisa Umeya, Izuru Miyawaki, Hiroshi Inada

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引用次数: 1

摘要

目的:我们研究了使用交流放大器检测视网膜电图(ERG)中代表视网膜色素上皮(RPE)功能的c波的条件，以及使用交流放大器记录的c波是否有助于评估RPE功能。方法:记录不同低频和不同亮度刺激下大鼠对5 s刺激的ERG反应。此外，我们还研究了静脉注射碘酸钠(SI)诱导RPE变性后ERGs的变化。结果:当低切滤波器频率为0.01 Hz，光刺激亮度≥- 1.0 log cd/m2时，c波检测清晰。注射后，c波比其他波(如a波和b波)衰减得更早。结论:使用设置为0.01 Hz的低切滤波器的交流放大器可以很容易地检测到c波。与传统的直流(DC)放大器相比，使用交流放大器可以更容易地记录c波，并且可以用于评估RPE功能。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Use of an alternating current amplifier when recording the ERG c-wave to evaluate the function of retinal pigment epithelial cells in rats.

Purpose: We studied the conditions under which c-waves of the electroretinogram (ERG), that represent retinal pigment epithelium (RPE) function, were detectable using an alternating current (AC) amplifier and whether the c-wave recorded using an AC amplifier was useful for evaluating RPE function.

Methods: We recorded ERG responses in rats to 5 s stimuli under the conditions in which the low-cut frequency and the stimulus luminance were varied. In addition, changes in ERGs were studied after intravenous injection of sodium iodate (SI) to induce RPE degeneration.

Results: The c-wave was detected clearly when the frequency of the low-cut filter was set at 0.01 Hz and light stimulus luminances were ≥ - 1.0 log cd/m². The c-wave was attenuated earlier than other waves (e.g., a-wave and b-wave) after SI administration.

Conclusions: The c-wave was easily detectable using an AC amplifier with the low-cut filter set at 0.01 Hz. Using the AC amplifier may allow easier c-wave recording, compared with the conventional use of a direct current (DC) amplifier, and could be useful for evaluating RPE function.

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来源期刊

Documenta Ophthalmologica 医学-眼科学

CiteScore

3.50

自引率

21.40%

发文量

审稿时长

>12 weeks

期刊介绍： Documenta Ophthalmologica is an official publication of the International Society for Clinical Electrophysiology of Vision. The purpose of the journal is to promote the understanding and application of clinical electrophysiology of vision. Documenta Ophthalmologica will publish reviews, research articles, technical notes, brief reports and case studies which inform the readers about basic and clinical sciences related to visual electrodiagnosis and means to improve diagnosis and clinical management of patients using visual electrophysiology. Studies may involve animals or humans. In either case appropriate care must be taken to follow the Declaration of Helsinki for human subject or appropriate humane standards of animal care (e.g., the ARVO standards on Animal Care and Use).