聚甘油脂酸酯包被胶原蛋白用于血管工程。

IF 1.2 Q3 CARDIAC & CARDIOVASCULAR SYSTEMS Journal of Cardiovascular and Thoracic Research Pub Date : 2022-01-01 Epub Date: 2022-09-06 DOI:10.34172/jcvtr.2022.31
Fateme Nazary Abrbekoh, Nasrin Valizadeh, Ayla Hassani, Hakime Ghale, Soltan Ali Mahboob, Reza Rahbarghazi, Ali Baradar Khoshfetrat, Mahdi Madipour
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引用次数: 2

摘要

在这里,我们监测了由聚甘油脂酸酯(PGS)包被胶原(Col)组成的支架在72小时后内皮细胞活性的细胞相容性。方法:将人内皮细胞分为对照组、PGS组和PGS+Col组。用FTIR和HNMR对支架进行了表征。接触角分析和扫描电镜研究了润湿性、表面形貌和细胞附着。LDH漏液法测定细胞存活率。采用western blotting和real-time PCR检测Tie-1、Tie-2、VE-Cadherin和VEGFR-2的水平。结果:FTIR和HNMR分析显示PGS+Col组混合适当。扫描电镜显示PGS组表面呈扁平状,PGS+Col组表面呈薄纤维状。在PGS中加入Col后,收缩角值从97.3˚降至81.1˚。与单独的PGS底物相比,在PGS+Col组中,细胞适当地附着在表面。与对照细胞相比,PGS和PGS+Col没有改变LDH向上清的渗漏,显示了PGS基支架的细胞相容性。结论:在不改变酪氨酸激酶受体和钙粘蛋白的情况下,同时应用Col和PGS可刺激正常内皮细胞形态。
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Combination of polyglycerol sebacate coated with collagen for vascular engineering.

Introduction: Here, we monitored the cytocompatibility of scaffolds consisting of poly (glycerol sebacate) (PGS) coated with collagen (Col) for endothelial cell activity after 72 hours. Methods: Human endothelial cells were allocated into Control, PGS, and PGS+Col groups. Scaffolds were characterized using FTIR and HNMR spectroscopy. Contact angel analysis and SEM were used to study wettability, surface morphology, and cell attachment. Cell survival was assessed using LDH leakage assay. Levels of Tie-1, Tie-2, VE-Cadherin, and VEGFR-2 were measured using western blotting and real-time PCR. Results: FTIR and HNMR analyses revealed the proper blending in PGS+Col group. SEM imaging exhibited a flat surface in the PGS group while thin Col fibers were detected in PGS+Col surface. The addition of Col to the PGS reduced the contract angle values from 97.3˚ to 81.1˚. Compared to PGS substrate alone, in PGS+Col group, cells appropriately attached to the surface. PGS and PGS+Col did not alter the leakage of LDH to the supernatant compared to control cells, showing the cytocopatiblity of PGS-based scaffolds. SOD and NO levels were increased significantly in PGS (p<0.05) and PGS+Col groups (p<0.001), respectively. We found that PGS+Col decreased Tie-1 content in endothelial cells whereas protein levels of Tie-2 and VE-Cadherin and expression of VEGFR-2 remained unchanged compared to PGS and control groups. Conclusion: Simultaneous application of Col and PGS can stimulate normal endothleial cell morphology without the alteration of tyrosine kinases receptors and cadherin.

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来源期刊
Journal of Cardiovascular and Thoracic Research
Journal of Cardiovascular and Thoracic Research CARDIAC & CARDIOVASCULAR SYSTEMS-
CiteScore
2.00
自引率
0.00%
发文量
22
审稿时长
7 weeks
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