人类疱疹病毒7型PCR检测方法的建立。

Q4 Health Professions Klinichescheskaya Laboratornaya Diagnostika Pub Date : 2022-11-14 DOI:10.51620/0869-2084-2022-67-11-658-662

S S Mardanly, S G Mardanly, A A Kazakov, V V Demkin, A M Zatevalov, A Yu Mironov

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引用次数: 1

摘要

一种聚合酶链反应(PCR)测定方法已被开发用于鉴定人类疱疹病毒7型的DNA。通过比较HHV-7全基因组核苷酸序列进行保守区搜索和选择。选择在HHV-7基因组中复制的片段作为扩增目标。在合成基质和临床样品上测试了该分析的性能。该方法灵敏度高，特异度高，在临床样品中检测HHV-7 DNA具有良好的效果。

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Development of a PCR assay for the detection of human herpes virus type 7.

A PCR assay has been developed to identify the DNA of the human herpes virus type 7. The search and selection of conserved regions was carried out by comparing the whole genome nucleotide sequences of HHV-7. A fragment duplicated in the HHV-7 genomes was chosen as a target for amplification. The performance of the assay was tested on a synthetic matrix and clinical samples. The developed assay has high sensitivity and specificity and showed good efficiency in detecting HHV-7 DNA in clinical samples.

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来源期刊

Klinichescheskaya Laboratornaya Diagnostika Health Professions-Medical Laboratory Technology

CiteScore

0.90

自引率

0.00%

发文量

110

期刊介绍： The journal deals with theoretical and practical problems of clinical laboratory diagnosis, publishes editorial articles, reviews of literature, original articles, short reports, discussions, book reviews, current events, materials which may assist the practitioners, methods of laboratory investigations used in medicine, materials on the results of practical application of new methods of investigation in the following fields of clinical laboratory diagnosis: hematology, cytology, coagulation, biochemistry, immunology.