{"title":"人白细胞溶酶体的体外分离及性质研究。","authors":"R HIRSCHHORN, G WEISSMANN","doi":"10.3181/00379727-119-30091","DOIUrl":null,"url":null,"abstract":"Summary A method has been described for isolation of human polymorphonuclear leukocyte lysosomes from 25 ml of peripheral venous blood. These granules resembled in content and behavior the granules of rabbit white cells and rat and rabbit liver. The “latent” acid hydrolases could be released from the granules by agents which have been shown to disrupt lysosomes from other tissues: streptolysins, lysolecithin, and etiocholanolone.","PeriodicalId":20675,"journal":{"name":"Proceedings of the Society for Experimental Biology and Medicine","volume":" ","pages":"36-9"},"PeriodicalIF":0.0000,"publicationDate":"1965-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3181/00379727-119-30091","citationCount":"26","resultStr":"{\"title\":\"ISOLATION AND PROPERTIES OF HUMAN LEUKOCYTE LYSOSOMES IN VITRO.\",\"authors\":\"R HIRSCHHORN, G WEISSMANN\",\"doi\":\"10.3181/00379727-119-30091\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Summary A method has been described for isolation of human polymorphonuclear leukocyte lysosomes from 25 ml of peripheral venous blood. These granules resembled in content and behavior the granules of rabbit white cells and rat and rabbit liver. The “latent” acid hydrolases could be released from the granules by agents which have been shown to disrupt lysosomes from other tissues: streptolysins, lysolecithin, and etiocholanolone.\",\"PeriodicalId\":20675,\"journal\":{\"name\":\"Proceedings of the Society for Experimental Biology and Medicine\",\"volume\":\" \",\"pages\":\"36-9\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1965-05-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.3181/00379727-119-30091\",\"citationCount\":\"26\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Proceedings of the Society for Experimental Biology and Medicine\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.3181/00379727-119-30091\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Proceedings of the Society for Experimental Biology and Medicine","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.3181/00379727-119-30091","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
ISOLATION AND PROPERTIES OF HUMAN LEUKOCYTE LYSOSOMES IN VITRO.
Summary A method has been described for isolation of human polymorphonuclear leukocyte lysosomes from 25 ml of peripheral venous blood. These granules resembled in content and behavior the granules of rabbit white cells and rat and rabbit liver. The “latent” acid hydrolases could be released from the granules by agents which have been shown to disrupt lysosomes from other tissues: streptolysins, lysolecithin, and etiocholanolone.
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