{"title":"细胞培养对血液的异常吸附。","authors":"R E NEUMAN, A A TYTELL","doi":"10.3181/00379727-119-30131","DOIUrl":null,"url":null,"abstract":"Summary Chicken RBC's in the presence of calcium ion or after treatment with metaperiodate were absorbed by cultured primary and stable line monkey kidney cells and to a lesser extent by rabbit kidney cells. An extensive variety of other primary or stable cell lines of human or animal origin did not adsorb the cells. Treatment of responsive cells with crystallized trypsin or chymotrypsin inhibited the hemadsorption. Apparently the anomalous hemadsorption was unrelated to viral infection. The morphology of attachment varied with cell strain. BSC-1 cells which displayed few cytoplasmic projections adsorbed RBC's through extensive contact in contrast to adsorption by MK-2 cells by microvillae which characterized the surface of these cells.","PeriodicalId":20675,"journal":{"name":"Proceedings of the Society for Experimental Biology and Medicine","volume":" ","pages":"180-6"},"PeriodicalIF":0.0000,"publicationDate":"1965-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3181/00379727-119-30131","citationCount":"1","resultStr":"{\"title\":\"ANOMALOUS HEMADSORPTION BY CELL CULTURES.\",\"authors\":\"R E NEUMAN, A A TYTELL\",\"doi\":\"10.3181/00379727-119-30131\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Summary Chicken RBC's in the presence of calcium ion or after treatment with metaperiodate were absorbed by cultured primary and stable line monkey kidney cells and to a lesser extent by rabbit kidney cells. An extensive variety of other primary or stable cell lines of human or animal origin did not adsorb the cells. Treatment of responsive cells with crystallized trypsin or chymotrypsin inhibited the hemadsorption. Apparently the anomalous hemadsorption was unrelated to viral infection. The morphology of attachment varied with cell strain. BSC-1 cells which displayed few cytoplasmic projections adsorbed RBC's through extensive contact in contrast to adsorption by MK-2 cells by microvillae which characterized the surface of these cells.\",\"PeriodicalId\":20675,\"journal\":{\"name\":\"Proceedings of the Society for Experimental Biology and Medicine\",\"volume\":\" \",\"pages\":\"180-6\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1965-05-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.3181/00379727-119-30131\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Proceedings of the Society for Experimental Biology and Medicine\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.3181/00379727-119-30131\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Proceedings of the Society for Experimental Biology and Medicine","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.3181/00379727-119-30131","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Summary Chicken RBC's in the presence of calcium ion or after treatment with metaperiodate were absorbed by cultured primary and stable line monkey kidney cells and to a lesser extent by rabbit kidney cells. An extensive variety of other primary or stable cell lines of human or animal origin did not adsorb the cells. Treatment of responsive cells with crystallized trypsin or chymotrypsin inhibited the hemadsorption. Apparently the anomalous hemadsorption was unrelated to viral infection. The morphology of attachment varied with cell strain. BSC-1 cells which displayed few cytoplasmic projections adsorbed RBC's through extensive contact in contrast to adsorption by MK-2 cells by microvillae which characterized the surface of these cells.