{"title":"木瓜蛋白酶处理内毒素的再激活。","authors":"J A RUDBACH, E RIBI, K C MILNER","doi":"10.3181/00379727-119-30112","DOIUrl":null,"url":null,"abstract":"Summary The pyrogenicity of endotoxin which had been diminished by incubation with papain was restored by treatment of the inactive complex with pronase and ethanol, indicating that a hydrolytic cleavage of the endotoxin molecule had not occurred. In addition, it was shown that the reduction of the second fever peak in papain-inactivated endotoxin could be explained on the basis of a normal dose-response phenomenon. These findings suggest that there is no disagreement between the inactivation of a purified endotoxin by papain and the observed correlation of the biological activity of endotoxin with the polysaccharide portion of the molecule.","PeriodicalId":20675,"journal":{"name":"Proceedings of the Society for Experimental Biology and Medicine","volume":" ","pages":"115-8"},"PeriodicalIF":0.0000,"publicationDate":"1965-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3181/00379727-119-30112","citationCount":"10","resultStr":"{\"title\":\"REACTIVATION OF PAPAIN-TREATED ENDOTOXIN.\",\"authors\":\"J A RUDBACH, E RIBI, K C MILNER\",\"doi\":\"10.3181/00379727-119-30112\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Summary The pyrogenicity of endotoxin which had been diminished by incubation with papain was restored by treatment of the inactive complex with pronase and ethanol, indicating that a hydrolytic cleavage of the endotoxin molecule had not occurred. In addition, it was shown that the reduction of the second fever peak in papain-inactivated endotoxin could be explained on the basis of a normal dose-response phenomenon. These findings suggest that there is no disagreement between the inactivation of a purified endotoxin by papain and the observed correlation of the biological activity of endotoxin with the polysaccharide portion of the molecule.\",\"PeriodicalId\":20675,\"journal\":{\"name\":\"Proceedings of the Society for Experimental Biology and Medicine\",\"volume\":\" \",\"pages\":\"115-8\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1965-05-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.3181/00379727-119-30112\",\"citationCount\":\"10\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Proceedings of the Society for Experimental Biology and Medicine\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.3181/00379727-119-30112\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Proceedings of the Society for Experimental Biology and Medicine","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.3181/00379727-119-30112","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Summary The pyrogenicity of endotoxin which had been diminished by incubation with papain was restored by treatment of the inactive complex with pronase and ethanol, indicating that a hydrolytic cleavage of the endotoxin molecule had not occurred. In addition, it was shown that the reduction of the second fever peak in papain-inactivated endotoxin could be explained on the basis of a normal dose-response phenomenon. These findings suggest that there is no disagreement between the inactivation of a purified endotoxin by papain and the observed correlation of the biological activity of endotoxin with the polysaccharide portion of the molecule.
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