含有d -半乳糖醛酸和磷酸残基的华葵中根瘤菌脂质A的研究。

Adam Choma, Pawel Sowinski
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引用次数: 34

摘要

研究了华奎中根瘤菌IFO 15243(T)游离脂质A的化学结构。脂质A是至少六种分子的混合物,其结构在糖骨架磷酸化和脂肪酰化上都不同。主链由β(1′->6)连接的2,3-二氨基-2,3-二脱氧葡萄糖(DAG)双糖组成,该双糖在4′位置部分被磷酸取代。dag -双糖的糖元被鉴定为α -d -半乳糖醛酸。所有脂质A种都携带4个酰胺连接的3-羟基脂肪残基。其中两个具有较短的烃链(即3-OH-i-13:0),而另外两个具有较长的烃链(即3- oh -i- 20:0)。3-羟基脂肪酸在还原和非还原DAG之间的分布是对称的。非极性以及(omega-1)羟基长链脂肪酸是酰基酰基部分的组成部分。两个酰基残基只存在于糖主链的非还原部分,但它们的分布尚未确定。远端DAG酰胺结合的脂肪酸羟基不被酯连接的酰基组分化学计量取代。
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Characterization of Mesorhizobium huakuii lipid A containing both D-galacturonic acid and phosphate residues.

The chemical structure of the free lipid A isolated from Mesorhizobium huakuii IFO 15243(T) was elucidated. Lipid A is a mixture of at least six species of molecules whose structures differ both in the phosphorylation of sugar backbone and in fatty acylation. The backbone consists of a beta (1'-->6) linked 2,3-diamino-2,3-dideoxyglucose (DAG) disaccharide that is partly substituted by phosphate at position 4'. The aglycon of the DAG-disaccharide has been identified as alpha-D-galacturonic acid. All lipid A species carry four amide-linked 3-hydroxyl fatty residues. Two of them have short hydrocarbon chains (i.e. 3-OH-i-13:0) while the other two have longer ones (i.e. 3-OH-20:0). Distribution of 3-hydroxyl fatty acids between the reducing and nonreducing DAG is symmetrical. The nonpolar as well as (omega-1) hydroxyl long chain fatty acids are components of acyloxyacyl moieties. Two acyloxyacyl residues occur exclusively in the nonreducing moiety of the sugar backbone but their distribution has not been established yet. The distal DAG amide-bound fatty acid hydroxyls are not stoichiometrically substituted by ester-linked acyl components.

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