{"title":"实验室制作100碱基对DNA分子量标记","authors":"Microsugar Chang, Jhih-Hao Wang, Han-Jung Lee","doi":"10.1016/j.jbbm.2007.08.002","DOIUrl":null,"url":null,"abstract":"<div><p>DNA molecular weight markers are routinely used in agarose gel electrophoresis. Here we report a method called PCR-synthesized marker (PSM) to generate DNA molecular rulers by PCR in the laboratory. This strategy can also be used to produce 100 bp RNA molecular weight markers by run-off transcription.</p></div>","PeriodicalId":15257,"journal":{"name":"Journal of biochemical and biophysical methods","volume":"70 6","pages":"Pages 1199-1202"},"PeriodicalIF":0.0000,"publicationDate":"2008-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.jbbm.2007.08.002","citationCount":"14","resultStr":"{\"title\":\"Laboratory production of 100 base pair DNA molecular weight markers\",\"authors\":\"Microsugar Chang, Jhih-Hao Wang, Han-Jung Lee\",\"doi\":\"10.1016/j.jbbm.2007.08.002\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>DNA molecular weight markers are routinely used in agarose gel electrophoresis. Here we report a method called PCR-synthesized marker (PSM) to generate DNA molecular rulers by PCR in the laboratory. This strategy can also be used to produce 100 bp RNA molecular weight markers by run-off transcription.</p></div>\",\"PeriodicalId\":15257,\"journal\":{\"name\":\"Journal of biochemical and biophysical methods\",\"volume\":\"70 6\",\"pages\":\"Pages 1199-1202\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2008-04-24\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/j.jbbm.2007.08.002\",\"citationCount\":\"14\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of biochemical and biophysical methods\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0165022X07001534\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of biochemical and biophysical methods","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0165022X07001534","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 14
摘要
DNA分子量标记通常用于琼脂糖凝胶电泳。本文报道了一种PCR合成标记(PSM)在实验室中通过PCR生成DNA分子标尺的方法。该策略也可用于通过径流转录产生100 bp RNA分子量标记。
Laboratory production of 100 base pair DNA molecular weight markers
DNA molecular weight markers are routinely used in agarose gel electrophoresis. Here we report a method called PCR-synthesized marker (PSM) to generate DNA molecular rulers by PCR in the laboratory. This strategy can also be used to produce 100 bp RNA molecular weight markers by run-off transcription.
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