O. I. Guliy, S. S. Evstigneeva, S. A. Staroverov, A. S. Fomin, O. A. Karavaeva
{"title":"卡那霉素噬菌体抗体的检测","authors":"O. I. Guliy, S. S. Evstigneeva, S. A. Staroverov, A. S. Fomin, O. A. Karavaeva","doi":"10.1134/S0003683823050071","DOIUrl":null,"url":null,"abstract":"<p>Recombinant antibodies specific to kanamycin were obtained using a sheep display library of scFv fragments (Griffin.1) and the possibility of their use for the determination of kanamycin by dot-immunoassay was demonstrated. The minimum detectable concentration of kanamycin was 1 μg/mL (distinguishable label binding other than background). It has been shown that anti-kanamycin phage antibodies are specific for kanamycin and do not interact with other antibiotics (neomycin, tetracycline, ampicillin, and gentamicin). Anti-kanamycin phage antibodies are a promising alternative to monoclonal antibodies for use in the determination of kanamycin.</p>","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"59 5","pages":"716 - 722"},"PeriodicalIF":1.1000,"publicationDate":"2023-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Phage Antibodies for Kanamycin Detection\",\"authors\":\"O. I. Guliy, S. S. Evstigneeva, S. A. Staroverov, A. S. Fomin, O. A. Karavaeva\",\"doi\":\"10.1134/S0003683823050071\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>Recombinant antibodies specific to kanamycin were obtained using a sheep display library of scFv fragments (Griffin.1) and the possibility of their use for the determination of kanamycin by dot-immunoassay was demonstrated. The minimum detectable concentration of kanamycin was 1 μg/mL (distinguishable label binding other than background). It has been shown that anti-kanamycin phage antibodies are specific for kanamycin and do not interact with other antibiotics (neomycin, tetracycline, ampicillin, and gentamicin). Anti-kanamycin phage antibodies are a promising alternative to monoclonal antibodies for use in the determination of kanamycin.</p>\",\"PeriodicalId\":466,\"journal\":{\"name\":\"Applied Biochemistry and Microbiology\",\"volume\":\"59 5\",\"pages\":\"716 - 722\"},\"PeriodicalIF\":1.1000,\"publicationDate\":\"2023-10-02\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Applied Biochemistry and Microbiology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://link.springer.com/article/10.1134/S0003683823050071\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Applied Biochemistry and Microbiology","FirstCategoryId":"99","ListUrlMain":"https://link.springer.com/article/10.1134/S0003683823050071","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
Recombinant antibodies specific to kanamycin were obtained using a sheep display library of scFv fragments (Griffin.1) and the possibility of their use for the determination of kanamycin by dot-immunoassay was demonstrated. The minimum detectable concentration of kanamycin was 1 μg/mL (distinguishable label binding other than background). It has been shown that anti-kanamycin phage antibodies are specific for kanamycin and do not interact with other antibiotics (neomycin, tetracycline, ampicillin, and gentamicin). Anti-kanamycin phage antibodies are a promising alternative to monoclonal antibodies for use in the determination of kanamycin.
期刊介绍：
Applied Biochemistry and Microbiology is an international peer reviewed journal that publishes original articles on biochemistry and microbiology that have or may have practical applications. The studies include: enzymes and mechanisms of enzymatic reactions, biosynthesis of low and high molecular physiologically active compounds; the studies of their structure and properties; biogenesis and pathways of their regulation; metabolism of producers of biologically active compounds, biocatalysis in organic synthesis, applied genetics of microorganisms, applied enzymology; protein and metabolic engineering, biochemical bases of phytoimmunity, applied aspects of biochemical and immunochemical analysis; biodegradation of xenobiotics; biosensors; biomedical research (without clinical studies). Along with experimental works, the journal publishes descriptions of novel research techniques and reviews on selected topics.
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