维持性血液透析中含柠檬酸A浓缩物的免疫表型特征:一项前后研究。

IF 1.7 Q3 UROLOGY & NEPHROLOGY International Journal of Nephrology Pub Date : 2023-09-27 eCollection Date: 2023-01-01 DOI:10.1155/2023/7772677
Yuli Shen, Christoph Schmaderer, Andreas Ossadnik, Arianne Hammitzsch, Javier Carbajo-Lozoya, Quirin Bachmann, Vera Bonell, Matthias Christoph Braunisch, Uwe Heemann, Dang Pham, Stephan Kemmner, Georg Lorenz
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引用次数: 0

摘要

引言:由于慢性炎症,维持性血液透析(MHD)患者继续表现出超额死亡率。不含醋酸盐的柠檬酸缓冲A浓缩物可以改善手术的生物相容性,减少慢性炎症,从而从长远来看改善患者的预后。方法:采用前后设计(3 醋酸几个月后3个月 在标准碳酸氢盐透析液血液透析(CiaHD)中的柠檬酸酸化A浓缩物的月数)和线性混合模型分析中,我们通过流式细胞术评估了CiaHD对外周T细胞和单核细胞计数和表型的影响。结果:改用CiaHD后,C反应蛋白(CRP)水平和白细胞计数未受影响。然而,CiaHD增加了离体淋巴细胞计数。此外,我们发现总CD3+CD4+CD69+((109/L),平均 ± SD:乙酸盐,0.04 ± 1.0相对于柠檬酸盐,0.02 ± 0.01;P = 0.02)活化的细胞而CD28+T细胞的数量保持稳定。在T细胞耗竭标志物表达、CD14+CD16+单核细胞计数和PMN-MDSCs方面没有发现差异。结论:与醋酸盐相比,CiaHD对淋巴细胞计数和CD4+T细胞活化的影响较小,这与全身CRP和电离镁、钙水平以及其他透析处方模式无关。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Immunophenotypic Characterization of Citrate-Containing A Concentrates in Maintenance Hemodialysis: A Pre-Post Study.

Introduction: Due to chronic inflammation, maintenance hemodialysis (MHD) patients continue to show excess mortality. Acetate-free citrate-buffered A concentrates could be a way to improve the biocompatibility of the procedure, reduce chronic inflammation, and thus in the long term improve the prognosis of patients.

Methods: Using a pre-post design (3 months of acetate followed by 3 months of citrate-acidified A concentrates in standard bicarbonate-based dialysate hemodialysis, CiaHD) and linear mixed model analysis in 61 stable HD patients, we assessed the impact of CiaHD on counts and phenotypes of peripheral T cells and monocytes by flow cytometry.

Results: Switching to CiaHD left C-reactive protein (CRP) levels and leucocyte counts unaffected. However, CiaHD increased lymphocyte counts ex vivo. Furthermore, we found a decrease in total CD3+CD4+CD69+ ((109/L), mean ± SD: acetate, 0.04 ± 1.0 versus citrate, 0.02 ± 0.01; P = 0.02) activated cells, while the number of CD28+ T cells remained stable. No differences were noted regarding T-cell exhaustion marker expression, CD14+CD16+ monocyte counts, and PMN-MDSCs.

Conclusion: Compared with acetate, CiaHD has a minor impact on lymphocyte counts and CD4+T-cell activation, which was independent of systemic CRP and ionized magnesium, calcium levels, and other dialysis prescription modalities.

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来源期刊
International Journal of Nephrology
International Journal of Nephrology UROLOGY & NEPHROLOGY-
CiteScore
3.40
自引率
4.80%
发文量
44
审稿时长
17 weeks
期刊介绍: International Journal of Nephrology is a peer-reviewed, Open Access journal that publishes original research articles, review articles, and clinical studies focusing on the prevention, diagnosis, and management of kidney diseases and associated disorders. The journal welcomes submissions related to cell biology, developmental biology, genetics, immunology, pathology, pathophysiology of renal disease and progression, clinical nephrology, dialysis, and transplantation.
期刊最新文献
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