疱疹病毒感染与IL10的相互作用及骨髓抑制的风险。

IF 0.3 Q4 TRANSPLANTATION International Journal of Organ Transplantation Medicine Pub Date : 2018-01-01 Epub Date: 2018-08-01
R Yaghobi, F Alizadeh, A Khodavandi
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引用次数: 0

摘要

背景:短暂性骨髓抑制综合征可能是由各种血液外疾病引起的,如免疫调节失调和继发性影响造血干细胞功能的病毒性传染病。目的:用IL10细胞因子基因多态性评价疱疹病毒的致病作用及其对短暂性骨髓抑制患者造血功能的影响。方法:在一项横断面研究中,招募了30名入住设拉子医科大学附属Namazi医院的短暂性骨髓抑制患者。血液科专家诊断为短暂性骨髓抑制。由100名健康的无关个体组成的对照组也包括在内。从每个研究患者中采集一份EDTA处理的血样,并分离血浆。分别使用实时和嵌套PCR方案评估巨细胞病毒和HHV8的分子流行率。采用PCR-RFLP方法对IL10(rs1800896-1082G/A)细胞因子基因的SNPs进行检测。结果:在短暂骨髓抑制的研究患者中,2例和3例发现巨细胞病毒和HHV8感染。与未感染者相比,HHV8感染者的IL10 G等位基因和GG基因型频率明显更高。与感染者相比,未感染巨细胞病毒的患者中IL10的A等位基因和AG和AA基因型的频率分别较高。与骨髓抑制的患者相比,在对照组中发现IL10 AA和AG基因型的频率显著更高。结论:IL10基因多态性可能在骨髓抑制患者对巨细胞病毒特别是HHV8感染的抵抗中起决定性作用。在未来的研究中,应通过对抗疱疹病毒免疫的分析来完成对骨髓抑制患者中HHV8感染和IL10基因之间新的相互作用的关注。
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Interaction between Herpes Virus Infections and IL10 and Risk of Bone Marrow Suppression.

Background: Syndrome of transient bone marrow suppression may result from various extra-hematological diseases, such as immunological deregulations, and viral infectious diseases secondarily affecting the function of hematopoietic stem cells.

Objective: To evaluate the pathogenic role of herpes viruses and their contraction with IL10 cytokine gene polymorphism, which can impair hematopoiesis in patients with transient bone marrow suppression.

Methods: In a cross-sectional study 30 patients who admitted to Namazi Hospital, affiliated to Shiraz University of Medical Sciences, with transient bone marrow suppression were recruited. Diagnosis of the transient bone marrow suppression was made by expert hematologists. A control group consisting of 100 healthy unrelated individuals was also included. One EDTA-treated blood sample was collected from each studied patients and plasma was isolated. The molecular prevalence of cytomegalovirus and HHV8 evaluated was evaluated using real-time and nested PCR protocols, respectively. The SNPs of the IL10 (rs 1800896-1082G/A) cytokine gene was evaluated by PCR-RFLP method.

Results: Cytomegalovirus and HHV8 infections were found in 2 and 3 of studied patients with transient bone marrow suppression. Significant higher frequency of IL10 G allele and GG genotype were found in HHV8-infected patients comparing to uninfected ones. Higher frequencies of A allele and AG and AA genotypes of IL10 were found in cytomegalovirus-uninfected patients comparing to infected ones, respectively. The significant higher frequencies of IL10 AA and AG genotypes were found in controls compared to bone marrow suppressed patients.

Conclusion: IL10 genetic polymorphism might have determinative role in resistance to the cytomegalovirus, especially HHV8 infections, in patients with bone marrow suppression. Focus in new interaction between HHV8 infection and IL10 genetics in bone marrow suppressed patients should be completed by the analysis of the anti-herpes virus immunity in future studies.

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来源期刊
CiteScore
1.60
自引率
0.00%
发文量
0
审稿时长
12 weeks
期刊介绍: The International Journal of Organ Transplantation Medicine (IJOTM) is a quarterly peer-reviewed English-language journal that publishes high-quality basic sciences and clinical research on transplantation. The scope of the journal includes organ and tissue donation, procurement and preservation; surgical techniques, innovations, and novelties in all aspects of transplantation; genomics and immunobiology; immunosuppressive drugs and pharmacology relevant to transplantation; graft survival and prevention of graft dysfunction and failure; clinical trials and population analyses in the field of transplantation; transplant complications; cell and tissue transplantation; infection; post-transplant malignancies; sociological and ethical issues and xenotransplantation.
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