Hox基因Ultrabithorax和腹肌-A在蝎子胚胎前体发育中的不同作用。

IF 2.3 2区 农林科学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Insect Molecular Biology Pub Date : 2023-10-04 DOI:10.1111/imb.12878
Bing-Peng Liu, Bao-Zhen Hua
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引用次数: 0

摘要

幼虫的腹部附肢有着复杂的得失进化史,但腹部附肢发育的调控机制在很大程度上仍不清楚。本文采用原位杂交和亲本RNA干扰的方法,研究了六花蝎蛛(Mecopera:Panorpidae)腹部前体的胚胎发生。结果表明,RNAi介导的Ultrabithorax(Ubx)敲低导致第一腹部节段(A1)向第三胸部节段(T3)的同源异型转化,并改变了下游靶向无远端(Dll)表达的分布,但不影响Dll的表达水平。敲除腹部A(abd-A)导致节段畸形、异常的prolegs和Dll表达中断。结果表明,基因Ubx在不阻止Dll启动的情况下维持了调节A1附属物命运的祖先作用,abd-a的二次适应进化出了指定腹部节段和proleg身份的能力。我们的结论是,在全代谢组幼虫的进化过程中,腹部Hox基因表达及其靶基因的变化调节着腹部附属物的形态。
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Distinct roles of the Hox genes Ultrabithorax and abdominal-A in scorpionfly embryonic proleg development

The abdominal appendages of larval insects have a complex evolutionary history of gain and loss, but the regulatory mechanisms underlying the abdominal appendage development remain largely unclear. Here, we investigated the embryogenesis of abdominal prolegs in the scorpionfly Panorpa liui Hua (Mecoptera: Panorpidae) using in situ hybridization and parental RNA interference. The results show that RNAi-mediated knockdown of Ultrabithorax (Ubx) led to a homeotic transformation of the first abdominal segment (A1) into the third thoracic segment (T3) and changed the distributions of the downstream target Distal-less (Dll) expression but did not affect the expression levels of Dll. Knockdown of abdominal-A (abd-A) resulted in malformed segments, abnormal prolegs and disrupted Dll expression. The results demonstrate that the gene Ubx maintains an ancestral role of modulating A1 appendage fate without preventing Dll initiation, and a secondary adaptation of abd-A evolves the ability to specify abdominal segments and proleg identity. We conclude that changes in abdominal Hox gene expression and their target genes regulate abdominal appendage morphology during the evolutionary course of holometabolous larvae.

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来源期刊
Insect Molecular Biology
Insect Molecular Biology 生物-昆虫学
CiteScore
4.80
自引率
3.80%
发文量
68
审稿时长
6-12 weeks
期刊介绍: Insect Molecular Biology has been dedicated to providing researchers with the opportunity to publish high quality original research on topics broadly related to insect molecular biology since 1992. IMB is particularly interested in publishing research in insect genomics/genes and proteomics/proteins. This includes research related to: • insect gene structure • control of gene expression • localisation and function/activity of proteins • interactions of proteins and ligands/substrates • effect of mutations on gene/protein function • evolution of insect genes/genomes, especially where principles relevant to insects in general are established • molecular population genetics where data are used to identify genes (or regions of genomes) involved in specific adaptations • gene mapping using molecular tools • molecular interactions of insects with microorganisms including Wolbachia, symbionts and viruses or other pathogens transmitted by insects Papers can include large data sets e.g.from micro-array or proteomic experiments or analyses of genome sequences done in silico (subject to the data being placed in the context of hypothesis testing).
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