{"title":"一种用于检测猫衣原体的重组酶辅助扩增试验。","authors":"Jian Liu, Weidong Qian, Jian Wang, Yilan Bai, Yaping Gui, Luming Xia, Guohua Gong, Feifei Ge, Haixiao Shen, Xiaojing Chang, Hongjin Zhao","doi":"10.33073/pjm-2023-029","DOIUrl":null,"url":null,"abstract":"<p><p><i>Chlamydia felis</i> is an important zoonotic agent for humans and various animals. A recombinase-aided amplification (RAA) assay was developed for detecting <i>C. felis</i>. RAA can be performed in a closed tube at 39°C within 30 min. The detection limit was 10.6 copies of the <i>C. felis</i> plasmid DNA per reaction. No positive signals for other pathogens were detected. The coincidence rate of RAA and conventional PCR was 95.24% (20/21) and 100% (96/96) for positive and negative samples, respectively. The established RAA assay is a simple, rapid, highly sensitive, and specific method for detecting <i>C. felis</i>.</p>","PeriodicalId":94173,"journal":{"name":"Polish journal of microbiology","volume":"72 3","pages":"339-343"},"PeriodicalIF":0.0000,"publicationDate":"2023-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/53/0f/pjm-72-3-pjm-2023-029.PMC10508970.pdf","citationCount":"0","resultStr":"{\"title\":\"A Recombinase-Aided Amplification Assay for the Detection of <i>Chlamydia felis</i>.\",\"authors\":\"Jian Liu, Weidong Qian, Jian Wang, Yilan Bai, Yaping Gui, Luming Xia, Guohua Gong, Feifei Ge, Haixiao Shen, Xiaojing Chang, Hongjin Zhao\",\"doi\":\"10.33073/pjm-2023-029\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p><i>Chlamydia felis</i> is an important zoonotic agent for humans and various animals. A recombinase-aided amplification (RAA) assay was developed for detecting <i>C. felis</i>. RAA can be performed in a closed tube at 39°C within 30 min. The detection limit was 10.6 copies of the <i>C. felis</i> plasmid DNA per reaction. No positive signals for other pathogens were detected. The coincidence rate of RAA and conventional PCR was 95.24% (20/21) and 100% (96/96) for positive and negative samples, respectively. The established RAA assay is a simple, rapid, highly sensitive, and specific method for detecting <i>C. felis</i>.</p>\",\"PeriodicalId\":94173,\"journal\":{\"name\":\"Polish journal of microbiology\",\"volume\":\"72 3\",\"pages\":\"339-343\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2023-09-20\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/53/0f/pjm-72-3-pjm-2023-029.PMC10508970.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Polish journal of microbiology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.33073/pjm-2023-029\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2023/9/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Polish journal of microbiology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.33073/pjm-2023-029","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2023/9/1 0:00:00","PubModel":"eCollection","JCR":"","JCRName":"","Score":null,"Total":0}
A Recombinase-Aided Amplification Assay for the Detection of Chlamydia felis.
Chlamydia felis is an important zoonotic agent for humans and various animals. A recombinase-aided amplification (RAA) assay was developed for detecting C. felis. RAA can be performed in a closed tube at 39°C within 30 min. The detection limit was 10.6 copies of the C. felis plasmid DNA per reaction. No positive signals for other pathogens were detected. The coincidence rate of RAA and conventional PCR was 95.24% (20/21) and 100% (96/96) for positive and negative samples, respectively. The established RAA assay is a simple, rapid, highly sensitive, and specific method for detecting C. felis.
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