柚木提取物对糖尿病大鼠一氧化氮和丙二醛水平的影响。

Q3 Agricultural and Biological Sciences Pakistan Journal of Biological Sciences Pub Date : 2023-03-01 DOI:10.3923/pjbs.2023.177.184
Parawansah, Nuralifah, Ruslin, Nurramadhani Armada Sida, Fadhliyah Malik, Atymutmain
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The animal models were divided into six treatment groups, normal control (KN), positive control (K&lt;sup&gt;+&lt;/sup&gt;) (glibenclamide), negative control (K&lt;sup&gt;&lt;/sup&gt;) (Na-CMC 0.5%), teak leaf ethanol extract group at a dose of D&lt;sub&gt;1&lt;/sub&gt; (100 mg kg&lt;sup&gt;1&lt;/sup&gt; b.wt.), D&lt;sub&gt;2&lt;/sub&gt; (200 mg kg&lt;sup&gt;1&lt;/sup&gt; b.wt.) and D&lt;sub&gt;3&lt;/sub&gt; (300 mg kg&lt;sup&gt;1&lt;/sup&gt; b.wt.). &lt;b&gt;Results:&lt;/b&gt; The antioxidant activity showed that the IC&lt;sub&gt;50&lt;/sub&gt; values of vitamin C and the ethanol extract of &lt;i&gt;Tectona grandis&lt;/i&gt; leaves were 18.208 and 62.236 μg mL&lt;sup&gt;1&lt;/sup&gt;, respectively. 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The MDA levels at KN = 3.767 μmol L&lt;sup&gt;1&lt;/sup&gt;, K&lt;sup&gt;+&lt;/sup&gt; = 8.854 μmol L&lt;sup&gt;1&lt;/sup&gt;, K&lt;sup&gt;&lt;/sup&gt; = 31.032 μmol L&lt;sup&gt;1&lt;/sup&gt;, D&lt;sub&gt;1&lt;/sub&gt; = 27.010 μmol L&lt;sup&gt;1&lt;/sup&gt;, D&lt;sub&gt;2&lt;/sub&gt; = 20.166 μmol L&lt;sup&gt;1&lt;/sup&gt; and D&lt;sub&gt;3&lt;/sub&gt; = 15.512 μmol L&lt;sup&gt;1&lt;/sup&gt;. &lt;b&gt;Conclusion:&lt;/b&gt; The ethanol extract of teak leaves &lt;i&gt;Tectona grandis&lt;/i&gt; L. at a dose of 300 mg kg&lt;sup&gt;1&lt;/sup&gt; reduces nitric oxide levels and plasma malondialdehyde levels in rats through the activity of antioxidant compounds.</p>","PeriodicalId":19800,"journal":{"name":"Pakistan Journal of Biological Sciences","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Effects of <i>Tectona grandis</i> L. 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引用次数: 0

摘要

<；b>；背景和目的：</b>；糖尿病的特征是胰腺β细胞紊乱引起的高血糖，导致活性氧的形成，在本研究中，糖尿病模型雄性Wistar大鼠的特征是一氧化氮和丙二醛水平升高<；b>；材料和方法：</b>；使用40mg/kg<；sup>；1</sup>；b.wt。腹腔注射链脲佐菌素。抗氧化活性基于铁还原抗氧化能力（FRAP）方法。分别使用Griess法和硫代巴比妥酸反应物质（TBARS）测定一氧化氮和MDA水平。将动物模型分为六个治疗组，正常对照组（KN）、阳性对照组（K<；sup>；+<；/sup>；）（格列本脲）、阴性对照组（K<；sub>；<；/sup>；sub>；1</sub>；（100mg kg<；sup>；1<；/sup>；b.wt.）、D<；sub>；2</sub>；（200mg kg<；sup>；1<；/sup>；b.wt.）和D<；sub>；3</sub>；（300mg kg<；sup>；1<；/sup>；b.wt.）；b>；结果：</b>；抗氧化活性表明，IC<；sub>；50</sub>；维生素C和<；i>；柚木</i>；叶片分别为18.208和62.236；sup>；1</sup>；，分别地mol L<；sup>；1</sup>；，K<；sup>+</sup>；=118.300μ；sup>；1</sup>；，K<；sup></sup>；=317.467μ；sup>；1</sup>；，D<；sub>；1</sub>；=210.133μ；sup>；1</sup>；，D<；sub>；2</sub>；=184.467μ；sup>；1</sup>；并且D<；sub>；3</sub>；=129.300μ；sup>；1</sup>；。在KN=3.767μ；sup>；1</sup>；，K<；sup>+</sup>；=8.854μ；sup>；1</sup>；，K<；sup></sup>；=31.032μ；sup>；1</sup>；，D<；sub>；1</sub>；=27.010μmol L<；sup>；1</sup>；，D<；sub>；2</sub>；=20.166μmol L<；sup>；1</sup>；并且D<；sub>；3</sub>；=15.512μ；sup>；1</sup><；b>；结论：</b>；柚木叶的乙醇提取物<；i>；柚木</i>；L.，剂量为300mg/kg<；sup>；1</sup>；通过抗氧化化合物的活性降低大鼠的一氧化氮水平和血浆丙二醛水平。
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Effects of Tectona grandis L. Extract in Diabetic Rats on Nitric Oxide and Malondialdehyde Levels.

<b>Background and Objective:</b> Diabetes mellitus is characterized by hyperglycemia caused by disturbances in pancreatic β cells resulting in the formation of reactive oxygen species which in this study was characterized by increased levels of nitric oxide and malondialdehyde in male Wistar rats with diabetes mellitus models. <b>Materials and Methods:</b> Animal modeling of diabetes mellitus using 40 mg kg<sup>1</sup> b.wt. streptozotocin intraperitoneally. The antioxidant activity is based on the ferric reducing antioxidant power (FRAP) method. Determination of nitric oxide and MDA levels using the Griess method and Thiobarbituric Acid Reactive Substances (TBARS) Assay, respectively. The animal models were divided into six treatment groups, normal control (KN), positive control (K<sup>+</sup>) (glibenclamide), negative control (K<sup></sup>) (Na-CMC 0.5%), teak leaf ethanol extract group at a dose of D<sub>1</sub> (100 mg kg<sup>1</sup> b.wt.), D<sub>2</sub> (200 mg kg<sup>1</sup> b.wt.) and D<sub>3</sub> (300 mg kg<sup>1</sup> b.wt.). <b>Results:</b> The antioxidant activity showed that the IC<sub>50</sub> values of vitamin C and the ethanol extract of <i>Tectona grandis</i> leaves were 18.208 and 62.236 μg mL<sup>1</sup>, respectively. The NO levels in KN = 83.133 μmol L<sup>1</sup>, K<sup>+</sup> = 118.300 μmol L<sup>1</sup>, K<sup></sup> = 317.467 μmol L<sup>1</sup>, D<sub>1</sub> = 210.133 μmol L<sup>1</sup>, D<sub>2</sub> = 184.467 μmol L<sup>1</sup> and D<sub>3</sub> = 129.300 μmol L<sup>1</sup>. The MDA levels at KN = 3.767 μmol L<sup>1</sup>, K<sup>+</sup> = 8.854 μmol L<sup>1</sup>, K<sup></sup> = 31.032 μmol L<sup>1</sup>, D<sub>1</sub> = 27.010 μmol L<sup>1</sup>, D<sub>2</sub> = 20.166 μmol L<sup>1</sup> and D<sub>3</sub> = 15.512 μmol L<sup>1</sup>. <b>Conclusion:</b> The ethanol extract of teak leaves <i>Tectona grandis</i> L. at a dose of 300 mg kg<sup>1</sup> reduces nitric oxide levels and plasma malondialdehyde levels in rats through the activity of antioxidant compounds.

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来源期刊
Pakistan Journal of Biological Sciences
Pakistan Journal of Biological Sciences Agricultural and Biological Sciences-Agronomy and Crop Science
CiteScore
1.90
自引率
0.00%
发文量
102
期刊介绍： Pakistan Journal of Biological Sciences (PJBS) is an international, peer-reviewed and well indexed scientific journal seeks to promote and disseminate the knowledge of biological sciences by publishing outstanding research in the field. Scope of the journal includes: Cell biology, developmental biology, structural biology, microbiology, entomology, toxicology, molecular biology & genetics, biochemistry, biotechnology, biodiversity, ecology, marine biology, plant biology and bioinformatics.
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