激光照射对切断和手术修复坐骨神经中胶质纤维酸性蛋白和同种异体炎症因子-1表达的影响。

International journal of physiology, pathophysiology and pharmacology Pub Date : 2023-08-15 eCollection Date: 2023-01-01
Zinovii Yashchyshyn, Marko Kozyk, Kateryna Strubchevska, Sergiy Ziablitsev
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引用次数: 0

摘要

目的:探讨不同光谱激光照射对坐骨神经再生过程中胶质纤维酸性蛋白(GFAP)和同种异体炎症因子-1(Iba-1)表达的影响。方法:实验在60只体重200-250g的实验室Wistar大鼠上进行。切断左侧坐骨神经,并在神经切断后10、20、30和45分钟进行端到端的表神经缝合。在神经修复90天后,通过多克隆抗GFAP抗体(Thermo Fisher Scientific,USA)和抗Iba-1抗体(Invitrogen,USA)进行蛋白质印迹和免疫组织化学分析。结果:无论损伤后进行手术神经修复的时间如何,使用绿色和蓝色光谱激光辐射都能显著增加GFAP蛋白的表达。波长为470nm的蓝光谱激光照射后,Iba-1和微管蛋白的表达显著高于对照值5.1-11.0倍。当使用波长为560nm的绿色光谱激光并且在神经损伤后10分钟和20分钟进行神经缝合时,注意到Iba-1和微管蛋白的表达增加。波长为520nm的绿色光谱激光对Iba-1和微管蛋白的表达没有显著影响。形态学上,当使用蓝光谱激光时,记录到胶质细胞的最高增殖反应。结论:蓝光(470nm)和绿光(560nm)激光照射能促进GFAP阳性许旺细胞的活化和神经再生。小胶质细胞的活化是神经再生的必要组成部分,Iba-1的含量代表了再生的效率。
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The effect of laser radiation on glial fibrillary acidic protein and allograft inflammatory factor-1 expression in severed and surgically repaired sciatic nerve.

Objective: Determine the effect of different spectrum laser radiations on the expression of Glial Fibrillary Acidic Protein (GFAP) and allograft inflammatory factor-1 (Iba-1) in the sciatic nerve during regeneration.

Methods: The experiment was performed on 60 lab Wistar rats weighing 200-250 g. The left sciatic nerve was severed and subsequent end-to-end epineural suturing was performed 10, 20, 30, and 45 minutes after neurotomy. Western blot and immunohistochemistry analyses were performed by means of polyclonal anti-GFAP antibodies (Thermo Fisher Scientific, USA) and anti-Iba-1 antibodies (Invitrogen, USA) 90 days after nerve repair.

Results: The use of green and blue spectrum laser radiation significantly increased GFAP protein expression regardless of the time when surgical nerve repair was performed after injury. The expression of Iba-1 and tubulin after blue spectrum laser radiation with a wavelength of 470 nm was significantly higher than the control values by 5.1-11.0 times. An increase in the expression of Iba-1 and tubulin was noted when a green spectrum laser with a wavelength of 560 nm was utilized and nerve suturing was performed 10 and 20 minutes after nerve injury. The green spectrum laser with a wavelength of 520 nm had no significant effect on the expression of Iba-1 and tubulin. Morphologically, the highest proliferative reaction of glia was recorded when using a blue spectrum laser.

Conclusions: Laser radiation with blue (470 nm) and green (560 nm) spectra, promoted the activation of GFAP-positive Schwann cells and nerve regeneration. Activation of microglia is a necessary component of nerve regeneration and the content of Iba-1 represented the efficiency of regeneration.

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