【RNAi抑制果蝇卵子发生过程中花生基因表达的表型表现分析】。

Genetika Pub Date : 2015-09-01
K A Akhmetova, C N Dorogova, I N Chesnokov, S A Fedorova
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引用次数: 0

摘要

研究了花生基因在果蝇卵子发生中的作用。研究表明,RNA干扰对花生在卵巢卵泡细胞中表达的抑制导致卵母细胞极化的破坏、绒毛膜细胞中的异常胞质分裂和卵泡细胞中的染色质凝聚的破坏。在花生基因表达降低或卵巢生殖细胞中Pnut蛋白缺失的雌性中没有观察到卵子发生侵犯。然而,这种雌性产生的胚胎的存活率下降是由两个死亡高峰引起的。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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[Analysis of Phenotypic Manifestation of peanut Gene Expression Suppression by RNAi in Drosophila Oogenesis].

The peanut gene functions in Drosophila melanogaster oogenesis were studied. It was demonstrated that the suppression of peanut expression by RNA interference in the ovary follicular cells results in the violation of oocyte polarization, anomalous cytokinesis in the chorion cells, and violation of the chromatin condensation in follicular cells. No oogenesis violations were observed in females with decreased peanut gene expression or an absence of the Pnut protein in the ovary generative cells. However, embryos produced by such females had a decreased survival rate caused by two death peaks.

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[Evaluation of Salt Tolerance of Transgenic Tobacco Plants Bearing with P5CS1 Gene of Arabidopsis thaliana]. [Analysis of Phenotypic Manifestation of peanut Gene Expression Suppression by RNAi in Drosophila Oogenesis]. [Transgenic tobacco (Nicotiana tabacum SR1) plants expressing the gene coding for Serratia marcescens nuclease]. [Level of phytohormones in various types of symbiotic pea mutants]. [Molecular-genetic identification and certification of soybean (Glycine max L.) cultivars].
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