Si Thu Aung, Saw Bawm, Hla Myet Chel, May June Thu, Soe Soe Wai, Yuki Eshita, Ryo Nakao, Ken Katakura, Lat Lat Htun
{"title":"利用缅甸线粒体细胞色素氧化酶亚基I基因对伊蚊、军蚊和库蚊的分子鉴定（直翅目：库蚊科）。","authors":"Si Thu Aung, Saw Bawm, Hla Myet Chel, May June Thu, Soe Soe Wai, Yuki Eshita, Ryo Nakao, Ken Katakura, Lat Lat Htun","doi":"10.1007/s11686-023-00721-x","DOIUrl":null,"url":null,"abstract":"<div><h3>Purpose</h3><p>Mosquitoes are important vectors that carry disease-causing agents that can affect humans and animals. DNA barcoding is a complementary identification which can be used to validate morphological characterization of mosquito species. The objectives of this study were to identify the mitochondrial sequence of the <i>COI</i> gene and to construct a molecular phylogeny based on the genetic divergence of the mosquito species studied.</p><h3>Methods</h3><p>In this study, DNA extraction and the amplification of the mitochondrial cytochrome oxidase subunit I genes (<i>COI</i>) were performed on pooled mosquito samples collected in Nay Pyi Taw area, Myanmar.</p><h3>Results</h3><p>Fragments of the <i>COI</i> gene showed 99–100% identity with sequences of <i>Aedes aegypti</i>, <i>Armigeres subalbatus</i>, <i>Culex pipiens</i> complex, and <i>Cx. quinquefasciatus</i>, respectively, deposited in GenBank. This study categorized two haplotypes from each <i>Ar. subalbatus</i> and <i>Cx. pipiens</i> complex <i>COI</i> gene sequence, as well as three haplotypes from <i>Cx. quinquefasciatus COI</i> gene sequences. The highest haplotype diversity and nucleotide diversity were observed in the <i>Ar. subalbatus</i> population (Hd = 1.0000; <i>π</i> = 0.0033), followed by the <i>Cx. pipiens</i> complex and <i>Cx. quinquefasciatus</i> populations.</p><h3>Conclusion</h3><p>This study provides useful information on the molecular identification and genetic diversity of mosquito vectors with medical and veterinary significance, which may assist in the improvement of mosquito control programs.</p></div>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"68 4","pages":"862 - 868"},"PeriodicalIF":1.2000,"publicationDate":"2023-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Molecular Identification of Aedes, Armigeres, and Culex Mosquitoes (Diptera: Culicidae) Using Mitochondrial Cytochrome Oxidase Subunit I Genes in Myanmar\",\"authors\":\"Si Thu Aung, Saw Bawm, Hla Myet Chel, May June Thu, Soe Soe Wai, Yuki Eshita, Ryo Nakao, Ken Katakura, Lat Lat Htun\",\"doi\":\"10.1007/s11686-023-00721-x\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Purpose</h3><p>Mosquitoes are important vectors that carry disease-causing agents that can affect humans and animals. DNA barcoding is a complementary identification which can be used to validate morphological characterization of mosquito species. The objectives of this study were to identify the mitochondrial sequence of the <i>COI</i> gene and to construct a molecular phylogeny based on the genetic divergence of the mosquito species studied.</p><h3>Methods</h3><p>In this study, DNA extraction and the amplification of the mitochondrial cytochrome oxidase subunit I genes (<i>COI</i>) were performed on pooled mosquito samples collected in Nay Pyi Taw area, Myanmar.</p><h3>Results</h3><p>Fragments of the <i>COI</i> gene showed 99–100% identity with sequences of <i>Aedes aegypti</i>, <i>Armigeres subalbatus</i>, <i>Culex pipiens</i> complex, and <i>Cx. quinquefasciatus</i>, respectively, deposited in GenBank. This study categorized two haplotypes from each <i>Ar. subalbatus</i> and <i>Cx. pipiens</i> complex <i>COI</i> gene sequence, as well as three haplotypes from <i>Cx. quinquefasciatus COI</i> gene sequences. The highest haplotype diversity and nucleotide diversity were observed in the <i>Ar. subalbatus</i> population (Hd = 1.0000; <i>π</i> = 0.0033), followed by the <i>Cx. pipiens</i> complex and <i>Cx. quinquefasciatus</i> populations.</p><h3>Conclusion</h3><p>This study provides useful information on the molecular identification and genetic diversity of mosquito vectors with medical and veterinary significance, which may assist in the improvement of mosquito control programs.</p></div>\",\"PeriodicalId\":6932,\"journal\":{\"name\":\"Acta Parasitologica\",\"volume\":\"68 4\",\"pages\":\"862 - 868\"},\"PeriodicalIF\":1.2000,\"publicationDate\":\"2023-10-13\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Acta Parasitologica\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://link.springer.com/article/10.1007/s11686-023-00721-x\",\"RegionNum\":3,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"PARASITOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Acta Parasitologica","FirstCategoryId":"3","ListUrlMain":"https://link.springer.com/article/10.1007/s11686-023-00721-x","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"PARASITOLOGY","Score":null,"Total":0}
Molecular Identification of Aedes, Armigeres, and Culex Mosquitoes (Diptera: Culicidae) Using Mitochondrial Cytochrome Oxidase Subunit I Genes in Myanmar
Purpose
Mosquitoes are important vectors that carry disease-causing agents that can affect humans and animals. DNA barcoding is a complementary identification which can be used to validate morphological characterization of mosquito species. The objectives of this study were to identify the mitochondrial sequence of the COI gene and to construct a molecular phylogeny based on the genetic divergence of the mosquito species studied.
Methods
In this study, DNA extraction and the amplification of the mitochondrial cytochrome oxidase subunit I genes (COI) were performed on pooled mosquito samples collected in Nay Pyi Taw area, Myanmar.
Results
Fragments of the COI gene showed 99–100% identity with sequences of Aedes aegypti, Armigeres subalbatus, Culex pipiens complex, and Cx. quinquefasciatus, respectively, deposited in GenBank. This study categorized two haplotypes from each Ar. subalbatus and Cx. pipiens complex COI gene sequence, as well as three haplotypes from Cx. quinquefasciatus COI gene sequences. The highest haplotype diversity and nucleotide diversity were observed in the Ar. subalbatus population (Hd = 1.0000; π = 0.0033), followed by the Cx. pipiens complex and Cx. quinquefasciatus populations.
Conclusion
This study provides useful information on the molecular identification and genetic diversity of mosquito vectors with medical and veterinary significance, which may assist in the improvement of mosquito control programs.
期刊介绍：
Acta Parasitologica is an international journal covering the latest advances in the subject.
Acta Parasitologica publishes original papers on all aspects of parasitology and host-parasite relationships, including the latest discoveries in biochemical and molecular biology of parasites, their physiology, morphology, taxonomy and ecology, as well as original research papers on immunology, pathology, and epidemiology of parasitic diseases in the context of medical, veterinary and biological sciences. The journal also publishes short research notes, invited review articles, book reviews.
The journal was founded in 1953 as "Acta Parasitologica Polonica" by the Polish Parasitological Society and since 1954 has been published by W. Stefanski Institute of Parasitology of the Polish Academy of Sciences in Warsaw. Since 1992 in has appeared as Acta Parasitologica in four issues per year.
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