p53干基因粉增强化疗对恶性胸膜间皮瘤的抗癌作用。

IF 4.6 3区医学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Gene Therapy Pub Date : 2023-10-13 DOI:10.1038/s41434-023-00424-y

Naomi Muramatsu, Misa Ichikawa, Tomoko Katagiri, Yumi Taguchi, Takashi Hatanaka, Tomoyuki Okuda, Hirokazu Okamoto

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引用次数: 0

摘要

基因干粉是一种新型的非病毒基因递送系统，可吸入，具有高基因表达。先前，我们发现用干基因粉末转染p16INK4a或TP53可在体外和体内抑制肺癌和恶性胸膜间皮瘤（MPM）的生长。在此，我们报道了含有p53表达质粒DNA的干基因粉末增强了顺铂（CDDP）对MPM的治疗作用，即使在内源性p53存在的情况下也是如此。此外，我们的结果表明，用更高浓度的质粒DNA（pDNA）安全转染抑制MPM的生长独立于化疗药物。为了为MPM患者开发一种新的治疗替代品，而不必担心“载体剂量”的安全性，我们的体外数据为干基因粉末提供了基本的理解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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p53 dry gene powder enhances anti-cancer effects of chemotherapy against malignant pleural mesothelioma

Dry gene powder is a novel non-viral gene-delivery system, which is inhalable with high gene expression. Previously, we showed that the transfection of p16INK4a or TP53 by dry gene powder resulted in growth inhibitions of lung cancer and malignant pleural mesothelioma (MPM) in vitro and in vivo. Here, we report that dry gene powder containing p53- expression-plasmid DNA enhanced the therapeutic effects of cisplatin (CDDP) against MPM even in the presence of endogenous p53. Furthermore, our results indicated that the safe transfection with a higher plasmid DNA (pDNA) concentration suppressed MPM growth independently of chemotherapeutic agents. To develop a new therapeutic alternative for MPM patients without safety concerns over “vector doses”, our in vitro data provide basic understandings for dry gene powder.

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来源期刊

Gene Therapy 医学-生化与分子生物学

CiteScore

9.70

自引率

2.00%

发文量

审稿时长

4-8 weeks

期刊介绍： Gene Therapy covers both the research and clinical applications of novel therapeutic techniques based on a genetic component. Over the last few decades, significant advances in technologies ranging from identifying novel genetic targets that cause disease through to clinical studies, which show therapeutic benefit, have elevated this multidisciplinary field to the forefront of modern medicine.